n(2)-n(2)-dimethylguanosine and Kidney-Failure--Chronic

n(2)-n(2)-dimethylguanosine has been researched along with Kidney-Failure--Chronic* in 1 studies

Trials

1 trial(s) available for n(2)-n(2)-dimethylguanosine and Kidney-Failure--Chronic

Article	Year
Characterization of dimethylguanosine, phenylethylamine, and phenylacetic acid as inhibitors of Ca2+ ATPase in end-stage renal failure. Journal of the American Society of Nephrology : JASN, 1998, Volume: 9, Issue:7 The activity of the plasma membrane Ca2+ ATPase of chronic renal failure patients is decreased by circulating inhibitors yet to be characterized. In this study, inhibitors of Ca2+ ATPase were isolated from ultrafiltrate of patients with end-stage renal failure. They were identified as dimethylguanosine, phenylethylamine, and phenylacetic acid by chromatography and mass spectrometry. Ca2+ ATPase activity was measured spectrophotometrically as the difference in hydrolysis of ATP in the presence and absence of Ca2+ with different concentrations of ATP and the isolated substances. All of the identified compounds are sufficiently lipophilic to penetrate the blood-brain barrier and to accumulate in cerebral tissue. The inhibitory effects of these agents were additive. The apparent K(m) values for ATP and Ca2+ were not altered by these substances, suggesting a noncompetitive mechanism of inhibition. In plasma of healthy subjects, the substances were not detectable. The Ca2+ ATPase inhibitors identified may play a role in the pathophysiology of end-stage renal failure and, potentially, in monitoring toxic effects on cellular Ca2+ metabolism in renal failure. Topics: Adult; Calcium-Transporting ATPases; Cell Membrane; Erythrocytes; Female; Guanosine; Humans; Kidney Failure, Chronic; Magnetic Resonance Spectroscopy; Male; Mass Spectrometry; Middle Aged; Phenethylamines; Phenylacetates; Reference Values; Statistics, Nonparametric	1998

Article

Year

Characterization of dimethylguanosine, phenylethylamine, and phenylacetic acid as inhibitors of Ca2+ ATPase in end-stage renal failure.

Journal of the American Society of Nephrology : JASN, 1998, Volume: 9, Issue:7

The activity of the plasma membrane Ca2+ ATPase of chronic renal failure patients is decreased by circulating inhibitors yet to be characterized. In this study, inhibitors of Ca2+ ATPase were isolated from ultrafiltrate of patients with end-stage renal failure. They were identified as dimethylguanosine, phenylethylamine, and phenylacetic acid by chromatography and mass spectrometry. Ca2+ ATPase activity was measured spectrophotometrically as the difference in hydrolysis of ATP in the presence and absence of Ca2+ with different concentrations of ATP and the isolated substances. All of the identified compounds are sufficiently lipophilic to penetrate the blood-brain barrier and to accumulate in cerebral tissue. The inhibitory effects of these agents were additive. The apparent K(m) values for ATP and Ca2+ were not altered by these substances, suggesting a noncompetitive mechanism of inhibition. In plasma of healthy subjects, the substances were not detectable. The Ca2+ ATPase inhibitors identified may play a role in the pathophysiology of end-stage renal failure and, potentially, in monitoring toxic effects on cellular Ca2+ metabolism in renal failure.

Topics: Adult; Calcium-Transporting ATPases; Cell Membrane; Erythrocytes; Female; Guanosine; Humans; Kidney Failure, Chronic; Magnetic Resonance Spectroscopy; Male; Mass Spectrometry; Middle Aged; Phenethylamines; Phenylacetates; Reference Values; Statistics, Nonparametric

1998