myxothiazol and Neoplasms

Hypoxia increased the ability of two human cancer cell lines, PC-3M and T24, to invade through Matrigel, while sodium nitroprusside (SNP), a nitric oxide (NO) donor, strongly inhibited this invasion, along with down-regulating HIF-1alpha. SNP also inhibited the function of mitochondria in PC-3M cells, and mitochondrion-specific inhibitors reduced the invasion of these cells. Furthermore, knocking down either Rieske iron-sulfur protein (Fe-S) of mitochondrial complex III or HIF-1beta in these cells decreased their invasive potential. Our findings suggest that NO inhibits invasion of cancer cells via both inhibition of HIF-1, and impairment of mitochondria.

Topics: Antimycin A; Cell Hypoxia; Cell Line, Tumor; Cell Movement; Cell Proliferation; Electron Transport Complex III; Humans; Hypoxia-Inducible Factor 1; Immunoblotting; Iron-Sulfur Proteins; Methacrylates; Mitochondria; Neoplasm Invasiveness; Neoplasms; Nitric Oxide; Nitric Oxide Donors; Nitroprusside; Oligomycins; RNA Interference; RNA, Small Interfering; Thiazoles; Transfection; Uncoupling Agents