myelin-basic-protein and Urinary-Bladder-Neoplasms

Leucocytes from 4 cancer patients showed cellular reactivity in the leucocyte adherence inhibition (LAI) assay in the presence of the synthetic encephalitogenic peptide of human myelin basic protein. All patients exhibited reactivity at a peptide concentration of 500 ng/ml. Leucocytes from 4 non-cancer patients failed to react. Suppression of LAI was detected in all 4 cancer patients by adding their serum to reactive mixtures containing peptide and autologous leucocytes. Each serum was subjected to column chromatography on Sephacryl S-200 to determine the molecular weight distribution of suppressive (blocking) factors. The greatest suppression was found in all cases within the range 90-155 kdalton.

Topics: Aged; Antigens, Neoplasm; Female; Humans; Immunity, Cellular; Immunologic Techniques; Intestinal Neoplasms; Leukocyte Adherence Inhibition Test; Lung Neoplasms; Lymphocytes; Middle Aged; Myelin Basic Protein; Neoplasms; Ovarian Neoplasms; Urinary Bladder Neoplasms

According to the present examinations it is pretty probable that already in early histological forms of the carcinoma of the urinary bladder the EM-test is able to indicate immunologically malignity, i.e. that the papillary non-infiltrating carcinoma of the transitional cell as well as the carcinoma in situ of the urinary bladder (23) also immunologically behave like tumors of the bladder. In differential-diagnostic questions concerning the organ localisation of the primary tumor the EM-test seems to give valuable references. Histologically not unequivocally malignant tumors of the bladder with positive results in the EM-test should be submitted to an exacter examination and control.

Topics: Adult; Aged; Antigens; Antigens, Neoplasm; Diagnosis, Differential; Humans; Male; Middle Aged; Myelin Basic Protein; Prostatic Neoplasms; Urinary Bladder Neoplasms

Fifty-four patients with malignant and other diseases of the urinary bladder and the prostate were examined for lymphocyte sensitization by means of electrophoretic mobility test (EM test). In this antigens from brain tissue (EF) and from malignomas of kidney, urinary bladder and prostate were used. Positive HEP values were obtained in 40 patients out of 42 with malignant tumours and in 5 patients out of 12 with other kidney diseases. With the corresponding tumour-associated antigens (TAA) in the EM-test, the histological tumour diagnosis could be confirmed in every case. Positive findings after using EF as well as the corresponding TAA are with great probability an indication of a malignant disease.

Topics: Adenocarcinoma; Adult; Aged; Antigens, Neoplasm; Brain; Cell Migration Inhibition; Electrophoresis; Humans; Kidney Diseases; Lymphocyte Activation; Male; Middle Aged; Myelin Basic Protein; Prostatic Neoplasms; Urinary Bladder Neoplasms