myelin-basic-protein and Ovarian-Neoplasms

The significance of neurospecific proteins in the diagnosis of neurotoxicity in patients with breast, lung, testicular, and ovarian cancer treated by taxane and cisplatin drugs was evaluated. The most pronounced increase in the content of these proteins and titers of autoantibodies to these proteins was observed in patients with clinical manifestations of neurotoxicity induced by cytostatics. A strong correlation was found between the concentration of myelin basic protein and cumulative dose of the drug (R=0.922; p<0.0001). These data suggest that myelin basic protein and gliofibrillar acid protein can be used as markers in the diagnosis and monitoring of antitumor drug neurotoxicity.

Topics: Antineoplastic Agents; Autoantibodies; Breast Neoplasms; Cisplatin; Dose-Response Relationship, Drug; Female; Glial Fibrillary Acidic Protein; Humans; Lung Neoplasms; Male; Myelin Basic Protein; Neurons; Ovarian Neoplasms; Testicular Neoplasms; Time Factors

Topics: Adult; Aged; Antigens, Neoplasm; Cell Migration Inhibition; Female; Genital Neoplasms, Female; Humans; Leukocytes; Middle Aged; Myelin Basic Protein; Ovarian Neoplasms

Leucocytes from 4 cancer patients showed cellular reactivity in the leucocyte adherence inhibition (LAI) assay in the presence of the synthetic encephalitogenic peptide of human myelin basic protein. All patients exhibited reactivity at a peptide concentration of 500 ng/ml. Leucocytes from 4 non-cancer patients failed to react. Suppression of LAI was detected in all 4 cancer patients by adding their serum to reactive mixtures containing peptide and autologous leucocytes. Each serum was subjected to column chromatography on Sephacryl S-200 to determine the molecular weight distribution of suppressive (blocking) factors. The greatest suppression was found in all cases within the range 90-155 kdalton.

Topics: Aged; Antigens, Neoplasm; Female; Humans; Immunity, Cellular; Immunologic Techniques; Intestinal Neoplasms; Leukocyte Adherence Inhibition Test; Lung Neoplasms; Lymphocytes; Middle Aged; Myelin Basic Protein; Neoplasms; Ovarian Neoplasms; Urinary Bladder Neoplasms

Monoclonal antibodies specific for neurofilament proteins, glial filament protein, or myelin basic protein were used with immunohistochemistry for evaluation of a series of 14 human benign and malignant teratomas for the presence of these neural specific antigens. The results indicate that human teratomas can express all of these neural antigens, reflecting the presence of differentiated neurons, astrocytes, and oligodendroglia, respectively. Although the tumors were selected because neural tissue had been noted on microscopic examination, 2 of the 15 cases lacked all of these neural antigens, and both were malignant teratomas. In the remaining cases, 2 or 3 of these neural antigens were detected. The presence of all 3 was correlated with the presence of mature, and the absence of immature, neural elements. The immunohistochemical detection of these antigens allows the confident recognition of neural elements in human teratomas, and their presence may be of prognostic significance.

Topics: Adolescent; Adult; Antibodies, Monoclonal; Antigens, Neoplasm; Astrocytes; Epitopes; Female; Histocytochemistry; Humans; Intermediate Filament Proteins; Male; Myelin Basic Protein; Neurons; Oligodendroglia; Ovarian Neoplasms; Teratoma; Testicular Neoplasms