myelin-basic-protein and Muscular-Diseases

A direct macrophage migration test is described, which proved to be useful for the detection of cellular hypersensitivity in man. We used a modification of the method described by Hughes & Paty (1972). In this method the MLR is abolished by 100 rad gamma-irradiation of the peritoneal exudate cells prior to pooling with human lymphocytes. Experiments with various intensity of irradiation, PPD, muscle antigen and encephalitogenic factor were performed to check this method. In a pilot study lymphocytes of patients with diseases of the central or peripheral nervous system or of muscle were tested. This proved that a hypersensitivity of Ef was present in various diseases of the CNS, while in muscle diseases positive tests were found using muscle antigen.

Topics: Antigens; Ascitic Fluid; Cell Migration Inhibition; Central Nervous System Diseases; Gamma Rays; Humans; Immunity, Cellular; Lymphocyte Culture Test, Mixed; Lymphocytes; Macrophages; Muscles; Muscular Diseases; Myelin Basic Protein; Peripheral Nervous System Diseases; Radiation Effects; Tuberculin; Tuberculin Test

Topics: Animals; Ataxia; Brain; Cattle; Desensitization, Immunologic; Encephalomyelitis, Autoimmune, Experimental; Feeding and Eating Disorders; Female; Haplorhini; Humans; Leukocytes; Macaca; Male; Meninges; Muscular Diseases; Myelin Basic Protein; Nerve Tissue Proteins; Neutrophils; Rabbits; Species Specificity; Spinal Cord