myelin-basic-protein and Mouth-Neoplasms

Nevic corpuscle (NC), a stacked lamellar structure reminiscent of Meissner corpuscle, is frequently observed in dermal melanocytic nevi. Although the heading 'neurotized' is classically used for these nevi, the exact neural nature of NC has been a topic of considerable debate. Neurotized nevi have received little attention in the dental literature, and there was no information on NC in oral melanocytic nevi.. Six cases of oral intramucosal nevi with a significant number of NC (two completely and four partially neurotized nevi) were examined immunohistochemically and ultrastructurally.. NC was composed of closely piled laminar cells devoid of visible melanin. NC and associated spindle nevus cells were immunopositive for S-100 protein but negative for HMB-45, myelin basic protein and epithelial membrane antigen. Within NC, no reactivity for neurofilament protein, protein gene product 9.5 or peripherin was evident. Numerous CD34-positive dendritic cells were located between nevus cells and often encircled NC. Ultrastructurally, NC consisted of concentrically layered elongated cells with a slender lamellated cytoplasm rich in thin filaments and pinocytotic vesicles. Their cytoplasmic processes were focally covered by external basal lamina and continuous to spindle nevus cells. Occasional NC cells contained a few melanosomes. There was no interposed axon in NC.. Despite the close resemblance to Meissner corpuscle, NC showed no axonal supply. NC cells lacked terminal Schwannian differentiation and appeared to be modified melanocytes with some perineurial ultrastructural characteristics. The presence of CD34-positive cells, presumably corresponding to endoneurial fibroblasts, further supports an organizational relationship of NC and peripheral nerve sheath elements.

Topics: Adult; Antigens, CD34; Antigens, Neoplasm; Cytoplasm; Dendritic Cells; Female; Humans; Immunohistochemistry; Intermediate Filament Proteins; Mechanoreceptors; Melanins; Melanocytes; Melanoma-Specific Antigens; Melanosomes; Membrane Glycoproteins; Middle Aged; Mouth Mucosa; Mouth Neoplasms; Mucin-1; Myelin Basic Protein; Neoplasm Proteins; Nerve Tissue Proteins; Neurofilament Proteins; Nevus; Peripherins; S100 Proteins; Ubiquitin Thiolesterase; Vacuoles

In a series of nine cases of intra-oral granular cell tumors (GCTs) an attempt was made, using both histochemical and immunocytochemical methods, to determine whether these tumours show Schwann-cell or neuroendocrine differentiation. Positive immunostaining with protein gene product 9.5 (PGP 9.5), neurone-specific enolase (NSE) and S-100, in contrast to predominantly negative immunostaining in 12 cases of neurilemmoma of the head and neck, and a similar pattern of staining with luxol fast blue to five known neuroendocrine tumours, strongly suggests that granular cell tumors may be neuroendocrine in nature.

Topics: Adolescent; Adult; Biomarkers, Tumor; Cell Lineage; Chromogranin A; Chromogranins; Diagnosis, Differential; Female; Granular Cell Tumor; Humans; Immunohistochemistry; Male; Middle Aged; Mouth Mucosa; Mouth Neoplasms; Myelin Basic Protein; Neuroendocrine Tumors; Phosphopyruvate Hydratase; S100 Proteins; Schwann Cells; Thiolester Hydrolases; Tongue Neoplasms; Ubiquitin Thiolesterase

The granular cell tumour (myoblastoma, Abrikosoff's tumour) and the congenital epulis in newborns (Neumann tumour) are two lesions rarely found in the oral cavity, whose histogenetic origin is highly controversial. This work analyses using immunohistochemical techniques 15 cases of myoblastomas and two of congenital epulis with different mono- and poly-clonal antibodies. Positive immunostaining was found for S-100 protein and neuron-specific enolase in all the cases of myoblastoma, and for vimentin and carcinoembryonic antigen in some cases. No immunoreactivity was observed for any of the other 13 antibodies used in congenital epulis.

Topics: Actins; Adolescent; Adult; Carcinoembryonic Antigen; Coloring Agents; Desmin; Female; Gingival Neoplasms; Granular Cell Tumor; Humans; Immunohistochemistry; Infant, Newborn; Keratins; Male; Mouth Neoplasms; Mucin-1; Myelin Basic Protein; Neoplasms, Muscle Tissue; Neurofilament Proteins; Phosphopyruvate Hydratase; S100 Proteins; Vimentin

Topics: Adult; Aged; Female; Humans; Immunologic Techniques; Leukocyte Adherence Inhibition Test; Male; Middle Aged; Mouth Neoplasms; Myelin Basic Protein