myelin-basic-protein and Arthritis

Topics: Animals; Arthritis; Autoimmune Diseases; Autoimmunity; B-Lymphocytes; Collagen; Encephalomyelitis, Autoimmune, Experimental; Humans; Multiple Sclerosis; Myelin Basic Protein; T-Lymphocytes

Topics: Animals; Arthritis; Autoimmunity; Cell Movement; Collagen Type II; Green Fluorescent Proteins; Joints; Luciferases; Mice; Myelin Basic Protein; T-Lymphocytes; Whole Body Imaging

DA rats develop transient arthritis after subcutaneous immunization with adjuvant-oil, while chronic arthritis and collagen autoreactivity ensues when collagen is added to the oil. We show here that DA rats can be protected from oil-induced arthritis (OIA) and rat collagen-induced arthritis (rCIA) by addition of antigen to these arthritogenic inocula. We have investigated this remarkable phenomenon and demonstrate that both foreign and self antigens can be protective, apparently provided they are immunogenic; hence HSP-65kDa, ovalbumin, rat myelin basic protein, rat IgG and bovine albumin are effective while rat albumin is not. This protection is long-lasting and disease-specific because rats protected from rCIA resist a later attempt to induce arthritis, but not experimental autoimmune encephalomyelitis (EAE). Protection from rCIA depends neither on the blocking of humoral autoreactivity to collagen nor on a change in the isotype profile of anti-collagen antibodies. We demonstrate that immunogens can also be protective when injected intraperitoneally only a few days before onset of arthritis. Our results indicate that protection is mediated through bystander immune reactions towards the co-immunized antigen and that the arthritogenicity of a given provocation, be it adjuvants, microbes or autoantigens, may be a complex net result of arthritogenic and contra-arthritogenic immune reactions.

Topics: Amino Acid Sequence; Animals; Antigens; Arthritis; Bacterial Proteins; Chaperonin 60; Chaperonins; Collagen; Encephalomyelitis, Autoimmune, Experimental; Female; Immunization; Immunoglobulin G; Male; Molecular Sequence Data; Myelin Basic Protein; Oils; Ovalbumin; Rats; Rats, Inbred Strains; Serum Albumin, Bovine

This article reviews some of the lessons derived from our use of T lymphocyte lines and clones to study three experimental autoimmune diseases: experimental autoimmune encephalomyelitis, experimental autoimmune thyroiditis and adjuvant arthritis. In each of these disease models we have raised lines and clones of T lymphocytes of the helper phenotype that are specifically autoimmune. These T lymphocytes were used to investigate the pathogenesis of disease. Lines and clones were also exploited to induce resistance to disease.

Topics: Adjuvants, Immunologic; Animals; Arthritis; Arthritis, Experimental; Autoimmune Diseases; Clone Cells; Disease Models, Animal; Encephalomyelitis, Autoimmune, Experimental; Mice; Myelin Basic Protein; Rats; T-Lymphocytes; Thyroglobulin; Thyroiditis, Autoimmune

Lines of rat T lymphocytes responsive to the basic protein of myelin (BP) or to the purified protein derivative of Mycobacterium tuberculosis (PPD) were inoculated i.v. into recipient rats. As reported previously, the anti-BP line cells, but not the anti-PPD line cells spontaneously accumulated in the central nervous system and caused encephalomyelitis. However, the anti-PPD line cells could be induced to enter the brain and cause bystander encephalitis by intracerebral inoculation of PPD. Anti-PPD or anti-BP line cells could mediate delayed-type hypersensitivity skin reactions or bystander arthritis elicited by specific antigen. The lines did not cause specific cytolysis in vitro. Susceptibility to delayed-type hypersensitivity or bystander disease was long lasting in rats inoculated with anti-PPD line cells, while rats inoculated with anti-BP line cells were susceptible for only a few days. Thus, lines of T lymphocytes can mediate a variety of pathological reactions directed by the presence of specific antigen, self or foreign.

Topics: Animals; Arthritis; Cell Line; Cytotoxicity, Immunologic; Dermatitis, Atopic; Encephalitis; Encephalomyelitis, Autoimmune, Experimental; Female; Hypersensitivity, Delayed; Injections, Intradermal; Macrophages; Male; Myelin Basic Protein; Rats; Rats, Inbred Lew; Skin Tests; T-Lymphocytes; Tuberculin