myelin-basic-protein and Antiphospholipid-Syndrome

The aim of the research was to study the content of myelin basic protein (MBP) in the cerebellum and cerebral hemispheres of the BALB/c mice with experimental antiphospholipid syndrome as well as in cases of introduction of L-arginine and aminoguanidine. The research was performed using 50 female BALB/c mice, in which APS was simulated. L-arginine (25 mg/kg) and aminoguanidine (10 mg/kg) were used for correction. A Western blot analysis of the main myelin protein (antibodies against MBP) as well as a densitometric analysis of immunoreactive zones was carried out using the samples of the cerebellum and cerebral hemispheres of the control and experimental BALB/c mice. The increased content of MBP (18.4 kDa) in 256 times (p<0.001) in the tissue of the cerebral hemispheres of the BALB/c mice with antiphospholipid syndrome was evidenced compare to the control. It was found out that in the samples of the cerebral hemispheres, the content of MBP (18.4 kDa) increased in 4.8 times (p<0.001) with the use of L-arginine, in 10 times (p<0.001) - with aminoguanidine, in 13 times (p<0.001) - in cases of the combined use of L-arginine and aminoguanidine compare to the indices of the mice with antiphospholipid syndrome. It was proved that the content of MBP (95-110 kDa) in the samples of the cerebral hemispheres of the experimental animals did not significantly change compare to the control. In the cerebellum of the animals with antiphospholipid syndrome, it was established that the level of MBP (95-110 kDa) increased in more than 5 times (p<0.001), while the MBP (18.4 kDa) was found in very small amounts compare to the control. In cases of lone and combined use of nitric oxide synthesis modulators (L-arginine and aminoguanidine), there were no significant changes in the level of MBP (95-110 kDa) in the cerebellum of BALB/c mice with antiphospholipid syndrome compare to the indices of the BALB/c mice with antiphospholipid syndrome only.

Topics: Animals; Antiphospholipid Syndrome; Arginine; Brain; Female; Mice; Mice, Inbred BALB C; Myelin Basic Protein; Nitric Oxide; Nitric Oxide Synthase