mycosubtiline and Hemolysis

mycosubtiline has been researched along with Hemolysis* in 2 studies

Other Studies

2 other study(ies) available for mycosubtiline and Hemolysis

Article	Year
Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism's antagonistic and biocontrol activities. Applied and environmental microbiology, 2005, Volume: 71, Issue:8 A Bacillus subtilis derivative was obtained from strain ATCC 6633 by replacement of the native promoter of the mycosubtilin operon by a constitutive promoter originating from the replication gene repU of the Staphylococcus aureus plasmid pUB110. The recombinant strain, designated BBG100, produced up to 15-fold more mycosubtilin than the wild type produced. The overproducing phenotype was related to enhancement of the antagonistic activities against several yeasts and pathogenic fungi. Hemolytic activities were also clearly increased in the modified strain. Mass spectrometry analyses of enriched mycosubtilin extracts showed similar patterns of lipopeptides for BBG100 and the wild type. Interestingly, these analyses also revealed a new form of mycosubtilin which was more easily detected in the BBG100 sample. When tested for its biocontrol potential, wild-type strain ATCC 6633 was almost ineffective for reducing a Pythium infection of tomato seedlings. However, treatment of seeds with the BBG100 overproducing strain resulted in a marked increase in the germination rate of seeds. This protective effect afforded by mycosubtilin overproduction was also visualized by the significantly greater fresh weight of emerging seedlings treated with BBG100 compared to controls or seedlings inoculated with the wild-type strain. Topics: Anti-Bacterial Agents; Antibiosis; Antifungal Agents; Bacillus subtilis; Culture Media; Gene Expression Regulation, Bacterial; Hemolysis; Lipoproteins; Microbial Sensitivity Tests; Micrococcus luteus; Pest Control, Biological; Plant Diseases; Promoter Regions, Genetic; Pythium; Solanum lycopersicum; Up-Regulation	2005
Action of mycosubtilin on erythrocytes and artificial membranes. Microbios, 1989, Volume: 59, Issue:240-241 Mycosubtilin has a strong lytic action upon erythrocytes. The haemolytic effect of the antibiotic is inhibited by free cholesterol as well as cholesterol included in liposomes of phosphatidylcholine-cholesterol. The antibiotic binding on artificial membranes was studied with radioactive bilayer vesicles of dipalmitoylphosphatidylcholine (DPPC), and DPPC-cholesterol:mycosubtilin destroyed both kinds of vesicles. The inhibition of the haemolytic effect of mycosubtilin by liposomes is a result of an interaction which diminishes the concentration of free cholesterol. Topics: 1,2-Dipalmitoylphosphatidylcholine; Antifungal Agents; Cholesterol; Erythrocytes; Hemolysis; Humans; In Vitro Techniques; Lipoproteins; Liposomes; Membranes, Artificial	1989

Article

Year

Mycosubtilin overproduction by Bacillus subtilis BBG100 enhances the organism's antagonistic and biocontrol activities.

Applied and environmental microbiology, 2005, Volume: 71, Issue:8

A Bacillus subtilis derivative was obtained from strain ATCC 6633 by replacement of the native promoter of the mycosubtilin operon by a constitutive promoter originating from the replication gene repU of the Staphylococcus aureus plasmid pUB110. The recombinant strain, designated BBG100, produced up to 15-fold more mycosubtilin than the wild type produced. The overproducing phenotype was related to enhancement of the antagonistic activities against several yeasts and pathogenic fungi. Hemolytic activities were also clearly increased in the modified strain. Mass spectrometry analyses of enriched mycosubtilin extracts showed similar patterns of lipopeptides for BBG100 and the wild type. Interestingly, these analyses also revealed a new form of mycosubtilin which was more easily detected in the BBG100 sample. When tested for its biocontrol potential, wild-type strain ATCC 6633 was almost ineffective for reducing a Pythium infection of tomato seedlings. However, treatment of seeds with the BBG100 overproducing strain resulted in a marked increase in the germination rate of seeds. This protective effect afforded by mycosubtilin overproduction was also visualized by the significantly greater fresh weight of emerging seedlings treated with BBG100 compared to controls or seedlings inoculated with the wild-type strain.

Topics: Anti-Bacterial Agents; Antibiosis; Antifungal Agents; Bacillus subtilis; Culture Media; Gene Expression Regulation, Bacterial; Hemolysis; Lipoproteins; Microbial Sensitivity Tests; Micrococcus luteus; Pest Control, Biological; Plant Diseases; Promoter Regions, Genetic; Pythium; Solanum lycopersicum; Up-Regulation

2005

Action of mycosubtilin on erythrocytes and artificial membranes.

Microbios, 1989, Volume: 59, Issue:240-241

Mycosubtilin has a strong lytic action upon erythrocytes. The haemolytic effect of the antibiotic is inhibited by free cholesterol as well as cholesterol included in liposomes of phosphatidylcholine-cholesterol. The antibiotic binding on artificial membranes was studied with radioactive bilayer vesicles of dipalmitoylphosphatidylcholine (DPPC), and DPPC-cholesterol:mycosubtilin destroyed both kinds of vesicles. The inhibition of the haemolytic effect of mycosubtilin by liposomes is a result of an interaction which diminishes the concentration of free cholesterol.

Topics: 1,2-Dipalmitoylphosphatidylcholine; Antifungal Agents; Cholesterol; Erythrocytes; Hemolysis; Humans; In Vitro Techniques; Lipoproteins; Liposomes; Membranes, Artificial

1989