mycophenolic-acid and Leukemia--T-Cell

To determine whether mycophenolic acid (MPA) exerts an apoptotic effect on human leukemic T cells Molt-4 and to elucidate its possible mechanism.. Cell morphology, DNA fragmentation, cell cycle,percentage of annexin V positive cells and enzymatic activity of caspase-3 were measured by microscopic, electrophoretic and flow cytometric techniques respectively. Human leukemic B cell line Raji was used as control.. MPA could induce apoptosis of Molt-4 cells with dose-and time-dependent manners; cells were blocked in the S phase. The activity of caspase-3 was enhanced in a dose-dependent manner in Molt-4 cells treated with MPA for 24 h. MPA could not induce apoptosis in Raji cells.. MPA can induce apoptosis in Molt-4 cells which may be involved in the activation of caspase-3.

Topics: Antibiotics, Antineoplastic; Apoptosis; Caspase 3; Caspases; Dose-Response Relationship, Drug; Humans; Leukemia, T-Cell; Mycophenolic Acid; Time Factors; Tumor Cells, Cultured