muramidase and Uterine-Cervical-Neoplasms

Topics: Adult; Clinical Trials as Topic; Condylomata Acuminata; Double-Blind Method; Female; Follow-Up Studies; Humans; Middle Aged; Muramidase; Time Factors; Uterine Cervical Neoplasms; Vaginal Creams, Foams, and Jellies

Adenoma malignum of the uterine cervix (mucinous type of minimal deviation adenocarcinoma, mucinous MDA), is a unique neoplasm that is difficult to diagnose owing to the deceptively benign appearance of the tumour cells. The present study was undertaken to explore the phenotypic expression of this tumour compared with those of non-neoplastic cervical tissues and of cervical carcinomas of various types. Ten cases of mucinous MDA, 50 cases with non-neoplastic cervical tissues, 13 of cervical adenocarcinoma including the mucinous (endocervical or intestinal type) and endometrioid types, and 2 of mucoepidermoid carcinoma were examined by various histochemical staining methods, including those for gastric mucins, pepsinogen, lysozyme, chromogranin A and carcinoembryonic antigen. The results revealed that mucinous MDA characteristically exhibited gastric phenotypes. The presence of gastric metaplasia was also demonstrated in 9 cases of mucinous MDA and in 5 of the other cases examined. The 7 endocervical-type adenocarcinomas also included 4 that expressed gastric phenotypes, and 2 of the 3 intestinal-type adenocarcinomas showed the same properties focally. These results indicate the presence of a group of lesions expressing gastric phenotypes in the uterine cervix and suggest a close relationship between these lesions. Cervical adenocarcinomas expressing gastric phenotypes are probably derived from MDA.

Topics: Adenocarcinoma, Mucinous; Adolescent; Adult; Chromogranin A; Chromogranins; Female; Histocytochemistry; Humans; Metaplasia; Middle Aged; Mucins; Muramidase; Neuraminic Acids; Pepsinogens; Stomach; Uterine Cervical Neoplasms

The present study evaluated the degree of renal impairment caused by intra-arterial hypertensive chemotherapy (CDDP, PEP). In 11 cases of advanced cancer of the uterine cervix, serum and urinary levels of alpha 1-microglobulin (alpha 1-m) and beta 2-microglobulin (beta 2-m), and urinary albumin (Alb) and lysozyme (LZM) were determined before the chemotherapy and 1,2 and 3 weeks after the therapy. Results are summarized as follows: 1. After intra-arterial chemotherapy, the histological classification was Grade I in 1 case (9.1%), Grade IIa in 2 cases (18.2%), and Grade IIb in 8 cases (72.7%). 2. Serum alpha 1-m and beta 2-m levels remained within the normal range after chemotherapy. 3. Urinary alpha 1-m, beta 2-m and LZM levels exceeded the normal limit between 1 and 2 weeks after the therapy, but thereafter they returned to normal. 4. Urinary Alb was significantly increased (p less than 0.05) between 1 and 2 weeks after therapy, but thereafter it returned to normal. These results suggested that intra-arterial chemotherapy (CDDP 100mg and PEP 40 mg in a dose) was effective for advanced cancer of the cervix and that renal disorders including tubular and glomerular impairment, which are the adverse effects of the therapy, were mild and reversible.

Topics: Adult; Aged; Albuminuria; Alpha-Globulins; Angiotensin II; Antineoplastic Combined Chemotherapy Protocols; beta 2-Microglobulin; Bleomycin; Blood Pressure; Carcinoma, Squamous Cell; Cisplatin; Female; Humans; Infusions, Intra-Arterial; Kidney; Middle Aged; Muramidase; Peplomycin; Uterine Cervical Neoplasms

Topics: Adenocarcinoma; Diagnosis, Differential; Female; Humans; Muramidase; Secretory Component; Uterine Cervical Neoplasms; Uterine Neoplasms

The numbers of Langerhans cells and lysozyme-positive macrophages were assessed quantitatively in normal ectocervical epithelium, cervical intraepithelial neoplasia (CIN), microinvasive squamous cell carcinoma (MICA), clinical invasive squamous cell carcinoma (CICA), and koilocytotic atypia using the avidin-biotin-peroxidase complex (ABC) method. The distribution of Langerhans cells was different from that of lysozyme-positive macrophages in that the former were intermingled with the cervical lesion, while the latter were present mainly surrounding the cervical lesion and/or on the edge of the cervical lesion. The numbers of Langerhans cells and lysozyme-positive macrophages in CIN were significantly larger than those in normal ectocervical epithelium and significantly smaller than those in invasive squamous cell carcinoma (MICA + CICA). Langerhans cell number significantly increased as the grade of CIN advanced. In contrast, the number of lysozyme-positive macrophages did not differ significantly between progressive grades of CIN. As for koilocytotic atypia, the numbers of Langerhans cells and lysozyme-positive macrophages in koilocytotic atypia were significantly greater than those in normal ectocervical epithelium but did not differ significantly from those in CIN 1 and CIN 2. With respect to stromal lymphoid infiltration, invasive squamous cell carcinoma with moderate or dense stromal lymphoid infiltration showed significantly greater numbers of Langerhans cells and lysozyme-positive macrophages than that with no or scattered stromal lymphoid infiltration, but such a correlation was not found in CIN.

Topics: Carcinoma, Squamous Cell; Epithelium; Female; Histiocytes; Humans; Immunoenzyme Techniques; Langerhans Cells; Muramidase; S100 Proteins; Uterine Cervical Neoplasms

In the literature you can find several therapeutic possibilities in the treatment of carcinoma by additive ozone therapy. We investigated the consequences of ozone therapy for the immunological status, lysozyme and vitamin A. 21 women with progressive cervical cancer (Stage III, IV) got besides the conventional irradiation therapy also an additive ozone therapy. After irradiation and ozone therapy a small decrease in IgG, IgA and IgM can be seen. A statistical significance could not be evaluated. There was no difference to the control group in lysozyme and vitamin A.

Topics: Carcinoma, Squamous Cell; Cobalt Radioisotopes; Combined Modality Therapy; Female; Humans; Immunoglobulins; Muramidase; Ozone; Radioisotope Teletherapy; Uterine Cervical Neoplasms; Vitamin A

The effects of lysozyme chloride (LY) and mainly on immunocompetence of patients with uterine cervical cancer were investigated partly in comparison with the historical control group. Patients were classified into non-surgery group (the LY group: 11 cases, the control group: 13 cases) and surgery group (the LY group: 6 cases, the control group: 7 cases). LY administration at a daily dose of 270 mg per os was started at the same time as the initiation of radiotherapy, and continued for three months after the therapy. 1) In non-surgery group composed of advanced cancer patients, a significant restorative effect of LY on a decrease in peripheral lymphocyte count induced by irradiation was observed. In surgery group composed of early stage of cancer patients, the restorative effect of LY was apparently mild. 2) The changes of other immunological parameters in the LY group such as lymphocyte blastogenic response to PHA, T, B-cell numbers and T-cell percent comparatively were well correlated with that of peripheral lymphocyte count in the LY group. 3) LY treatment resulted in a significant suppression of post-radiative decrease in leucocytes count in non-surgery group. 4) LY treatment tended to bring about the improvement of depressed state of trophicity and liver function especially of advanced cancer patients. From these results, it was suggested that LY might act as a kind of immunomodulator, and lead to the potentiation of depressed immunological status of advanced uterine cervical cancer patients. It was therefore expected that adjuvant LY treatment could contribute to the advantageous clinical effects in the treatment of cancer patients.

Topics: Adult; Aged; Aspartate Aminotransferases; Blood Proteins; Female; Humans; Leukocyte Count; Lymphocyte Activation; Lymphocytes; Middle Aged; Muramidase; Serum Albumin; Uterine Cervical Neoplasms

Topics: Dinitrochlorobenzene; Female; Humans; Immunity; Immunoglobulin A; Immunoglobulin G; Muramidase; Skin Tests; Streptodornase and Streptokinase; Tuberculin Test; Uterine Cervical Neoplasms

Serum lysozyme has been demonstrated to be an indicator for macrophage activity in the tumor-bearing host. Therefore, we investigated lysozyme levels in the sera of 336 untreated tumor patients (121 malignant melanoma, 61 lung cancers, 70 cervical cancers, 49 breast cancers and 35 benign breast tumors, and 36 healthy controls). Patients with malignant melanoma and lung cancer had significantly higher lysozyme levels than the healthy controls. Within the clinical stages in melanoma, there was a decrease of lysozyme in stages II and III in comparison to stage I, but still above that of the control values. Patients with benign breast tumors had normal levels, whereas in breast cancer patients of stages I and II there was a significant reduction in the lysozyme levels. In stages III and IV no differences to the control group could be detected. In patients with cervical cancer (FIGO II and III) serum lysozyme levels were found to be within the normal range. From this study it can not be concluded that serum lysozyme reflects the immunological reactivity of the tumor bearer. Nevertheless, the reduced levels in stages I and II of breast cancer might point to an immunological defect.

Topics: Breast Neoplasms; Female; Humans; Lung Neoplasms; Melanoma; Muramidase; Neoplasms; Uterine Cervical Neoplasms

74 patients with untreated cervical cancer (FIGO II and III) were skintested with a battery of recall antigens, and also sensitised and challenged with DNCB. A significant reduction of reaction to Tuberkulin, Varidase and also to DNCB was found in patients with stage III in comparison to healthy females of the same age group. Significant changes in immunoglobulin levels, increase of IgA and decrease of IgG were observed in the cancer patients. Serum lysozyme values were the same as in the control group.

Topics: Carcinoma, Squamous Cell; Dinitrochlorobenzene; Female; Humans; Hypersensitivity, Delayed; Immunity; Immunoglobulin A; Immunoglobulin G; Muramidase; Skin Tests; Uterine Cervical Neoplasms

Infusion of cycloheximide i.v., an antibiotic known to inhibit synthesis of protein, at a rate of 0.2 mg/kg/hr, reliably caused lysis of fever in 15 chronically febrile patients with Hodgkin's disease who did not have detectable bacterial, fungal, or viral infection. Antipyretic effects were also seen in some patients with reticulum cell sarcoma, lymphosarcoma, acute leukemia, histiocytic medullary reticulosis, plasma cell myeloma, carcinoma of the lung, and carcinoma of the cervix. The drug failed to produce defervescence in four patients with normal granulocyte reserves, who were febrile due to bacterial infection. When infused at a rate of 0.2 mg/kg/hr, the drug apparently caused an acute alteration of protein metabolism in man in that plasma amino acid nitrogen rose acutely while plasma levels of muramidase and ribonuclease fell during the period of the infusion. The data suggest that continuing synthesis of protein may be involved in nonbacterial fever of neoplastic disease. Mammalian granulocytes and monocytes are known to elaborate a pyrogenic protein following appropriate stimulation; it is suggested that in some types of neoplastic disease, particularly Hodgkin's disease, tumor cells may produce and release a pyrogenic protein and that drug-induced inhibition of its synthesis is responsible for the observed lysis of fever.

Topics: Bacterial Infections; Cycloheximide; Female; Fever; Hodgkin Disease; Humans; Leukemia; Lung Neoplasms; Lymphatic Diseases; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Multiple Myeloma; Muramidase; Neoplasm Proteins; Neoplasms; Nitrogen; Ribonucleases; Uterine Cervical Neoplasms

Topics: Antineoplastic Agents; Cyclophosphamide; Cytostatic Agents; Female; Humans; Leukopenia; Muramidase; Toxicology; Triaziquone; Uterine Cervical Neoplasms