muramidase and Urinary-Tract-Infections

Topics: Acetates; Acetic Acid; Animals; Anti-Bacterial Agents; Anti-Infective Agents, Local; Anti-Infective Agents, Urinary; Edetic Acid; Humans; Muramidase; Rats; Therapeutic Irrigation; Urinary Bladder; Urinary Tract Infections

Forty-one patients with urinary tract infections were randomly assigned to receive for six days gentamicin, amikacin, sisomicin or netilmicin. The dose for each patient was calculated according to creatinine clearance and lean body mass in order to avoid overdosages. Urinary enzymes (alpha-glucosidase, gamma-glutamyltranspeptidase and muramidase), serum creatinine and creatinine clearance, proteinuria and urinary sediment were evaluated for nephrotoxicity. None of the patients developed nephrotoxicity, but urinary enzymes rose significantly in all. The statistical analysis of enzymuria during the treatment permitted the definition of a rank order of the nephrotoxic potential of the aminoglycosides studied.

Topics: Adolescent; Adult; Aged; alpha-Glucosidases; Amikacin; Creatinine; Female; gamma-Glutamyltransferase; Gentamicins; Glucosidases; Humans; Kanamycin; Kidney; Male; Middle Aged; Muramidase; Netilmicin; Proteinuria; Sisomicin; Urinary Tract Infections

To perform an experimental evaluation of the effect of Phytofron, used for the treatment of urinary tract infections, on the ability of opportunistic pathogens to inactivate innate immunity factors (lysozyme, pro- and anti-inflammatory cytokines) and form biofilms.. In vitro experiments were carried out on clinical isolates from urine of patients with pyelonephritis and cystitis: Escherichia coli, Staphylococcus aureus, S. haemolyticus, S. epidermidis, Enterococcus faecalis. The effect of Fitofron NPO FarmVILAR (Russia) on the anticytokine activity of bacteria against regulatory cytokines (IL4, IL6, IL8, TNF and IL17A) was determined by enzyme immunoassay, while anti-lysozyme trait and the ability to form biofilms was evaluated by the photometric method.. The inhibitory effect of Fitofron on the ability of opportunistic microorganisms to inactivate innate immunity factors (lysozyme) and form biofilms, as well as the predominant inhibition of the studied cytokines, was experimentally established.. Inhibition of the persistence factors of opportunistic pathogens by Fitofron, documented in vitro, can be considered as one of the possible mechanisms of its biological activity in vivo.

Topics: Anti-Bacterial Agents; Bacteria; Biofilms; Cytokines; Escherichia coli; Humans; Muramidase; Pyelonephritis; Urinary Tract Infections

The desideratum aim of the present context was to isolate a promising antagonist probiotic bacterium from fermented food item as biocontrol agent against uropathogens. Among diversified isolates evaluated for antagonistic trait, Staphylococcus succinus strain AAS2 was found to be an auspicious candidate against urinary tract infection (UTI) causing bacterial pathogens, being the most active against Staphylococcus aureus with substantial activity of 352.5 ± 5.4 AU/mL. Further, the in vitro probiotic attributes of strain AAS2 were assessed using systematic methodology. The isolate exhibited tolerance to acidic condition (up to pH 3.0) and simulated gastric juice (at pH 3.0) with fairly high survival logarithmic cell counts of 5.3 ± 0.15 and 5.23 ± 0.02 log cfu/mL, respectively. Additionally, strain AAS2 showed capability to resist 0.5% w/v bile salt too. It also revealed significant values of auto-aggregation (32.5 ± 1.3-56.5 ± 1.4%) and cell surface hydrophobicity (38.35 ± 1.4%) properties. The isolate showed resistivity towards phenol (6.8 ± 0.08 log cfu/mL) and lysozyme (58.6 ± 1.6%). Further, the susceptibility trait of strain AAS2 to conventional antibiotics made this isolate a promising probiotic bacterium. Most importantly, the isolate depicted DPPH (2,2-Diphenyl-1-picrylhydrazyl) and hydroxyl radical scavenging activities in a concentration dependent manner, thereby exhibiting its propitious antioxidative properties. In a nutshell, the outcomes of this investigation divulge the plausible use of S. succinus strain AAS2 as biocontrol agent against uropathogens, and recommended further applications in pharmaceutics due to its pronounced probiotic traits.

Topics: Anti-Bacterial Agents; Antibiosis; Bile Acids and Salts; Biphenyl Compounds; DNA, Bacterial; Fermented Foods; Food Microbiology; Gastric Juice; Humans; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Hydroxyl Radical; India; Microbial Sensitivity Tests; Microbial Viability; Muramidase; Phenol; Picrates; Probiotics; Staphylococcus; Staphylococcus aureus; Urinary Tract Infections

In 26 patients suffering from rheumatoid arthritis complicated by urinary tract infections the normal lysozyme activity has been observed in the blood serum. After 14 days lasting therapy with nonsteroid antiinflammatory drugs no changes in the enzyme activity have been observed.

Topics: Adult; Aged; Anti-Inflammatory Agents, Non-Steroidal; Arthritis, Rheumatoid; Female; Humans; Male; Middle Aged; Muramidase; Urinary Tract Infections

Topics: beta 2-Microglobulin; Dilatation, Pathologic; Female; Humans; Infant, Newborn; Kidney Calices; Muramidase; Pregnancy; Pregnancy Complications, Infectious; Prospective Studies; Urinary Tract Infections

Topics: Adult; Aged; Diabetes Mellitus, Type 1; Diabetes Mellitus, Type 2; Enzyme Activation; Humans; Middle Aged; Muramidase; Urinary Tract Infections

Topics: Aged; Diabetic Nephropathies; Humans; Kidney Failure, Chronic; Kidney Function Tests; Leukocyte Count; Muramidase; Reagent Kits, Diagnostic; Urinary Tract Infections

Topics: Animals; Animals, Laboratory; Coagulase; Food Microbiology; Humans; Mice; Milk; Muramidase; Staphylococcal Infections; Staphylococcus; Urinary Tract Infections; Virulence

The presence of tubular involvement, as a marker for the detection of urinary tract infection (UTI) site, was examined in 19 patients with pyelonephritis and in 15 patients with cystitis or asymptomatic bacteriuria. The urinary excretion of four markers of tubular proteinuria, beta 2-microglobulin (beta 2M), lysozyme (LZ), lactic dehydrogenase isoenzyme V (LAD-5) and N-acetyl-beta D-glucosaminidase (NAG), was investigated. LAD-5 appeared particularly valuable for the early detection of upper UTI. However, the overall diagnostic accuracy appeared to be further strengthened using, besides LAD-5, one additional variable. A set of simple and noninvasive biochemical tests on urine samples can reliably help to identify the site of UTI.

Topics: Acetylglucosaminidase; Adult; beta 2-Microglobulin; Clinical Enzyme Tests; Humans; Isoenzymes; Kidney Diseases; Kidney Tubules; L-Lactate Dehydrogenase; Muramidase; Proteinuria; Urinary Tract Infections

Topics: Bacterial Infections; Child; Colitis, Ulcerative; Crohn Disease; Diagnosis, Differential; Feces; Hepatitis A; Humans; Meningitis; Meningitis, Aseptic; Muramidase; Urinary Tract Infections; Virus Diseases

Topics: Bacteriuria; Female; Humans; Male; Muramidase; Pyuria; Urinary Tract Infections

Urinary levels of lysozyme activity were measured in 32 children afflicted with acute or chronic urinary tract infection and compared to a group of 30 healthy subjects. A significant difference in the levels of lysozyme activity between the two groups could be observed. In the subjects with acute urinary tract infection the lysozyme levels in the urine were additionally determined at the beginning of the therapy, on the third day, the tenth day and three days after cessation of therapy. High lysozyme levels encountered at the onset of the infection showed under therapy a clear tendency to decrease and in all cases no lysozyme was present three days after therapy was completed. The possible causes of the pathological lysozymuria are discussed. The determination of lysozyme is thus an additional method by which to control the course and outcome of urinary tract infections.

Topics: Acute Disease; Adolescent; Child; Child, Preschool; Chronic Disease; Female; Humans; Infant; Male; Muramidase; Recurrence; Urinary Tract Infections

Topics: Acute Disease; Adolescent; Age Factors; Child; Child, Preschool; Humans; Muramidase; Urinary Tract Infections

Topics: Humans; Muramidase; Urinary Tract Infections

Topics: gamma-Glutamyltransferase; Glomerulonephritis; Humans; Kidney Tubules; Muramidase; Urinary Tract Infections

Urinary excretion of lysozyme was investigated in a group of 66 patients with various renal diseases, nephrolitiasis and urinary tract infections. The results obtained demonstrate that the amount of the enzyme excreted is related to the entity of tubular damage whereas is not with glomerular damage. No correlation was found between lysozyme excretion neither to the degree of proteinuria neither to the amount of leukocytes and bacteria in the urine. In patients with urinary infections urinary lysozyme increases only when there is a tubular injury of some entity. In 90 pediatric patients with urinary infection and pyelonephritis lysozyme in the urine was found only in two cases. Therefore urinary lysozyme determination cannot be considered for the detection of early tubular injury and is not a helpful diagnostic tool in urinary tract infections.

Topics: Adolescent; Adult; Aged; Child; Child, Preschool; Female; Humans; Infant; Kidney Diseases; Male; Middle Aged; Muramidase; Urinary Tract Infections

Topics: Animals; Bacteriuria; Cystitis; Dog Diseases; Dogs; Edetic Acid; Gentamicins; Injections, Subcutaneous; Male; Muramidase; Pseudomonas aeruginosa; Pseudomonas Infections; Therapeutic Irrigation; Tromethamine; Urinary Catheterization; Urinary Tract Infections

Topics: Acidosis, Renal Tubular; Adolescent; Adult; Blood Chemical Analysis; Calcium; Child; Child, Preschool; Female; Glomerular Filtration Rate; Humans; Hydrogen-Ion Concentration; Immunoglobulins; Kidney Concentrating Ability; Kidney Tubules, Proximal; Male; Molecular Weight; Muramidase; Nephrocalcinosis; Pedigree; Proteins; Proteinuria; Renal Aminoacidurias; Urinary Calculi; Urinary Tract Infections; Urine

Topics: Coagulase; Cytosine; DNA, Bacterial; Guanine; Humans; Microbial Sensitivity Tests; Micrococcus; Muramidase; Protein Denaturation; Staphylococcus; Temperature; Ultracentrifugation; Urinary Tract Infections

Topics: Edetic Acid; Enterobacteriaceae Infections; Humans; Muramidase; Pseudomonas Infections; Therapeutic Irrigation; Tromethamine; Urinary Bladder Diseases; Urinary Tract Infections

Topics: Cerebrospinal Fluid Shunts; Coagulase; Humans; Muramidase; Sepsis; Staphylococcal Infections; Staphylococcus; Urinary Tract Infections; Wound Infection

Topics: Adult; Child; Coagulase; Heart Valve Prosthesis; Humans; Micrococcus; Muramidase; Postoperative Complications; Skin; Staphylococcus; Urinary Tract Infections