muramidase and Tuberculosis--Meningeal

Topics: Antibody Specificity; Antigens, Bacterial; Enzyme-Linked Immunosorbent Assay; Humans; Immunoglobulins; Lymphocyte Activation; Muramidase; Mycobacterium tuberculosis; Rosette Formation; Tuberculosis, Meningeal

Lysozyme activity was assayed in the cerebrospinal fluid (CSF) of 32 tuberculous meningitis (TBM), 17 bacterial meningitis, 10 partially treated bacterial meningitis, 18 encephalitis and 18 control subjects. The mean CSF lysozyme activity was significantly raised (p < 0.001) in TBM patients compared with other study groups. A cut-off CSF lysozyme level of > or = 26 U/l had a sensitivity and specificity of 93.7 and 84.1 per cent, respectively for the diagnosis of TBM. Overall, it was found to be a better test than any other single test and thus can be used for rapid and early diagnosis of TBM in children.

Topics: Case-Control Studies; Child; Child, Preschool; Diagnosis, Differential; Encephalitis; Humans; India; Infant; Muramidase; Sensitivity and Specificity; Tuberculosis, Meningeal

The adenosine deaminase (ADA), lysozyme (LZM) and lactate dehydrogenase (LDH) of serum and cerebrospinal fluid (CSF) were determined in 36 patients with tuberculous meningitis (TBM), 47 patients with non-tuberculous meningitis (N-T-BM) and 20 patients with non-central nervous system diseases(control group). The results showed that the assessment of serum and CSF ADA and LZM activity may be helpful to the differential diagnosis between TBM and N-TBM.

Topics: Adenosine Deaminase; Adolescent; Adult; Clinical Enzyme Tests; Diagnosis, Differential; Female; Humans; L-Lactate Dehydrogenase; Male; Middle Aged; Muramidase; Tuberculosis, Meningeal

Topics: Adolescent; Adult; Aged; Child; Child, Preschool; Diagnosis, Differential; Female; Humans; Male; Middle Aged; Muramidase; Tuberculosis, Meningeal

Topics: Clinical Enzyme Tests; Humans; Muramidase; Tuberculosis, Meningeal

Topics: Adolescent; Child; Humans; Muramidase; Mycobacterium tuberculosis; Tuberculosis, Meningeal

The significance of the measurement of lactate, lysozyme and PHI in CSF for differential diagnosis of meningitis was examined in 58 cases of viral, 36 of bacterial and 5 of tuberculous etiology. In the early phase of the illness CSF lactate was found to be the most sensitive parameter for distinction of viral from bacterial or tuberculous meningitis respectively. Except for one case CSF lactate exceeded 3.8 mmol/l in all cases of bacterial etiology, whereas this value was never reached in any case of viral meningitis. While lactate concentration was maximal on the day of admission and declined continuously thereafter, PHI activity reached its maximum on the third day after beginning of the therapy. At this time all patients with a bacterial or tuberculous meningitis had PHI activities about 50 U/l. This value wasn't exceeded in any case of viral meningitis. In a few cases some days after onset of therapy a distinction of bacterial meningitis from viral forms was still possible by PHI determination but not by lactate measurement. Determination of lysozyme also could be helpful in the later phase of the disease.

Topics: Bacterial Infections; Cryptococcosis; Diagnosis, Differential; Glucose-6-Phosphate Isomerase; Humans; Lactates; Lactic Acid; Meningitis; Meningitis, Viral; Muramidase; Mycoplasma Infections; Tuberculosis, Meningeal

Topics: Diagnosis, Differential; Humans; Macrophages; Meningitis; Muramidase; Tuberculosis, Meningeal

Topics: Humans; Muramidase; Pleural Effusion; Tuberculosis, Meningeal; Tuberculosis, Pleural

Lysozyme was determined with a turbidimetric assay in blood and cerebrospinal fluid. There were 17 children without CNS-disease, 30 patients with viral meningitis, 5 patients with encephalitis, 3 with symphatetic meningitis, 3 with Guillain Barré syndrome, 25 children with bacterial meningitis, 1 patient with tuberculous meningitis and two with intracranial tumors. The lysozyme level was below 0.5 microgram Hl/ml in patients without CNS-disease, in viral and symphatetic meningitis, Guillain Barré syndrome and encephalitis. Elevated lysozyme levels were found in bacterial meningitis, in the one case with tuberculous meningitis and in brain tumors (Ependymoma and Medulloblastoma). In our opinion the lysozyme level is a valuable aid in the differentiation of viral meningitis to bacterial meningitis.

Topics: Cerebellar Neoplasms; Cerebrospinal Fluid; Child; Ependymoma; Humans; Medulloblastoma; Meningitis; Meningoencephalitis; Muramidase; Tuberculosis, Meningeal