muramidase and Stomatitis--Denture

Little information is available about the acquired pellicle layer that is formed on denture surfaces or its role in regulating microbial colonization of the prosthetic surface. Because denture-induced stomatitis is associated with increased numbers of Candida albicans and other microorganisms on the denture surface, the acquired denture pellicle (ADP) may play a role in modulating this colonization. This study examined and compared ADP from healthy patients and patients with stomatitis by chemical and immunochemical methods. The ADP was found to be composed of a selectively adsorbed layer containing salivary amylase, high molecular weight mucin (MG1), lysozyme, albumin, and sIgA. Salivary cystatins, proline-rich proteins, and low molecular weight mucin (MG2) were not detected. ADP amino acid composition was distinct from any of the ductal salivas, but had many similarities with enamel pellicle. Immunoblots of ADP from patients with stomatitis identified additional serum components, degradation products, and C. albicans cell components that were not detected in ADP from healthy patients. Quantification of these molecules in ADP could lead to a diagnostic test for oral mucosal disease underlying a denture base. Identification of specific molecules in denture pellicle that promote adhesion of C. albicans may elucidate a mechanism of fungal cell colonization on the denture surface. Future studies that chemically modify the denture acrylic resin surface to immobilize antimicrobial proteins may be a means of decreasing pathogenic plaque development.

Topics: Adhesiveness; Aged; Albumins; alpha-Amylases; Amino Acids; Candida albicans; Dental Deposits; Dental Pellicle; Denture, Complete, Upper; Female; Humans; Immunoglobulin A, Secretory; Male; Middle Aged; Molecular Weight; Mucins; Muramidase; Saliva; Salivary Proteins and Peptides; Stomatitis, Denture

Topics: Candida; Candidiasis, Oral; Candidiasis, Vulvovaginal; Female; Humans; Microbial Sensitivity Tests; Muramidase; Stomatitis, Denture

Topics: Aged; Antibody Formation; Denture, Complete; Humans; Immunoglobulin A, Secretory; Immunoglobulins; Middle Aged; Muramidase; Saliva; Stomatitis; Stomatitis, Denture

Topics: Adult; Aged; Candidiasis, Oral; Female; Humans; Hydrogen-Ion Concentration; Male; Middle Aged; Muramidase; Saliva; Stomatitis; Stomatitis, Denture