muramidase and Pulmonary-Embolism

The role of free radical generation and its scavenging enzymes in circulating mice polymorphonuclear leukocytes (PMNLs) has been studied following pulmonary thromboembolism. Levels of malonaldehyde (MDA), 02- radical generation, activity of superoxide dismutase (SOD), catalase (CAT), lactate dehydrogenase (LDH), myeloperoxidase (MPO) and lysozyme were estimated in lysed neutrophil preparations. Activities of SOD and CAT were increased in neutrophils, while animals showed 60 +/- 4% thrombocytopenia. Levels of MDA in PMNLs were also elevated significantly following thrombosis. However, there was no significant change in superoxide radical generation, after thrombotic challenge, in mice neutrophils. The present study provides evidence for the involvement of free radicals in mice pulmonary thromboembolism.

Topics: Animals; Catalase; Collagen; Epinephrine; Free Radical Scavengers; Free Radicals; Leukocyte Count; Lipid Peroxidation; Male; Malondialdehyde; Mice; Muramidase; Neutropenia; Neutrophils; Peroxidase; Pulmonary Embolism; Superoxide Dismutase; Thrombocytopenia