muramidase and Pre-Eclampsia

The incidence of preeclampsia is high in northern Nigeria, as it is in many other developing countries, and preeclampsia is associated with significant maternal and fetal morbidity and mortality. We inquired if proteinuria or hypertension alone could account for the altered concentrations of urinary lysosomal hydrolases that have been reported in preeclamptic women and pregnant women without preeclampsia.. The activities of urinary beta-hexosaminidase and beta-galactosidase were determined fluorometrically in pregnant women assigned to one of four groups: Group I: 41 preeclamptic women; Group II: 31 hypertensive aproteinuric women; Group III: 44 normotensive proteinuric women; and Group IV: 52 healthy pregnant women (controls).. The urinary beta-hexosaminidase concentrations were decreased in the preeclamptic women (P<0.005) and proteinuric women (P<0.001) when compared to the healthy pregnant controls. There was no significant difference in beta-hexosaminidase concentrations between the hypertensive women and the healthy pregnant controls. The urinary beta-galactosidase concentrations for preeclamptic, hypertensive, and proteinuric women did not differ significantly versus healthy pregnant controls.. The reduced urinary excretion of beta-hexosaminidase in preeclamptic women is associated with proteinuria, but not hypertension. Measuring urinary concentrations of lysosomal hydrolases alone or in conjunction with urinary protein concentrations is not likely to be useful in predicting or monitoring the clinical course of preeclampsia; however, it might prove important in gaining a more complete understanding of the pathogenesis of renal tubular epithelial cell injury and proteinuria that occurs in preeclampsia.

Topics: beta-Galactosidase; beta-N-Acetylhexosaminidases; Case-Control Studies; Female; Humans; Lysosomes; Muramidase; Nigeria; Pre-Eclampsia; Pregnancy

To study the clinical significance of changes of N-acetyl-beta-D-glucosaminidase (NAG) and lysozyme in random urine sample in pregnancy induced hypertension (PIH).. The concentration of NAG was measured by oronitrophenol spectrophotometric methods, and the concentration of lysozyme by agar plate diffusion method. Random urine samples from 266 pregnant women were examined, 110 cases were normal pregnant women, 156 cases were PIH patients.. (1) The level of NAG and lysozyme in moderate and severe PIH patients were significantly higher than that in mild PIH patients and normal pregnant women (P < 0.01) and it increased with the severity of disease. (2) There was a positive correlation between NAG and protein/creatinine (Pr/Cr) ratio in random urine. (3) There was a positive correlation between urinary lysozyme and serum beta 2-microglobulin (r = 0.874, P < 0.05).. Determination of urinary NAG and lysozyme levels will differentiate various states of PIH.

Topics: Acetylglucosaminidase; Adult; beta 2-Microglobulin; Creatinine; Female; Humans; Muramidase; Pre-Eclampsia; Pregnancy

Topics: Adult; Antimicrobial Cationic Peptides; Blood Bactericidal Activity; Blood Proteins; Complement System Proteins; Female; Humans; Muramidase; Phagocytosis; Postpartum Period; Pre-Eclampsia; Pregnancy; Pregnancy Trimester, Third; Proteins

To characterize the cellular and humoral immune situation in pregnancies with EPH-gestosis (n = 44), percentages of peripheral T-lymphocytes, ratio of helper and suppressor T-lymphocytes further circulating immuncomplexes (CIC), beta 2-Microglobulin (beta 2-MG) and lysozyme (Lz) were determined. 50 pregnant women without EPH-gestosis were examined as controls. The mean counts of total lymphocytes decreased with increasing gestosis index in contrast to T-lymphocyte percentages. The mean T-helper/T-suppressorcell ratio indicated a severe imbalance in a few cases with severe gestosis. CIC were present in all serum samples of patients with moderate and severe gestosis. Lz was found to be elevated in EPH-gestosis in correlation to severity of disease. beta 2-MG levels in serum were only increased in women with reduced renal function. This parameter was not strictly correlated to the severity of EPH-gestosis but only to the degree of kidney dysfunctioning. EPH-gestosis represents a heterogeneous group with uniform symptomatology. Only in severe gestosis an imbalance of T-lymphocyte subpopulations as well as CIC and elevated Lz levels were detected. In mild gestosis no alterations of the used parameters were found.

Topics: Antigen-Antibody Complex; beta 2-Microglobulin; Female; Humans; Leukocyte Count; Muramidase; Pre-Eclampsia; Pregnancy; Pregnancy Trimester, Third; T-Lymphocytes

Topics: Complement System Proteins; Female; Humans; Immunity, Innate; Immunoglobulins; Infant, Newborn; Maternal-Fetal Exchange; Muramidase; Phagocytosis; Pre-Eclampsia; Pregnancy; Pregnancy Trimester, Third; Properdin

Topics: Female; Humans; Infant, Newborn; Muramidase; Pre-Eclampsia; Pregnancy

Topics: Adult; Female; Humans; Hypertension; Kidney Diseases; Muramidase; Pre-Eclampsia; Pregnancy