muramidase and Nephritis

To determine if changes in dietary protein intake alter renal excretion of small molecular weight proteins in passive Heymann nephritis, 21 rats with passive Heymann nephritis were fed 8.5% protein for 12 days after injection with antiserum. Dietary protein intake was then increased to 40% in 10 rats (LP-HP) while 11 rats remained on 8.5% protein (LP-LP). Lysozymuria (UlysV) increased from 66.5 +/- 31.0 mcg/day to 457.5 +/- 98.0 mcg/day (P less than 0.001) after five days in LP-HP, but was unchanged in LP-LP. Albuminuria (UalbV) increased only in LP-HP, from 168 +/- 23 mg/day to 447 +/- 45 mg/day (P less than 0.001). Urinary lysozyme excretion correlated with UalbV (r = 0.737, P less than 0.001), and changes in UlysV correlated with changes in UalbV (r = 0.657, P less than 0.01). To determine whether the increase in UlysV was the direct effect of the change in diet, enalapril 40 mg/kg/day was administered to prevent the increase in UalbV that occurs when these rats are fed a high protein diet. Twelve rats were fed 8.5% (LP) and 10 were fed 40% protein (HP) from the time of injection with antiserum. Six LP (LPE) and five HP (HPE) received enalapril. UlysV was 873 +/- 391 mcg/day in HP and nearly undetectable in the other three groups. UalbV was significantly greater in HP (368 +/- 60 mg/day) compared to the other three groups (114 +/- 16 in LP, 136 +/- 44 in HPE, 95 +/- 21 in LPE.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Albuminuria; Animals; Dietary Proteins; Enalapril; Glomerular Filtration Rate; Male; Muramidase; Nephritis; Rats; Rats, Inbred Strains

Topics: Adolescent; beta 2-Microglobulin; Child; Child, Preschool; Electrophoresis; Hemagglutination Tests; Humans; Immunoenzyme Techniques; Immunoglobulin Fragments; Immunoglobulin G; Muramidase; Nephritis; Radioimmunoassay

The effect of reduced glomerular anionic charge on immune complex deposition in the glomerular basement membrane was studied in rats with Heymann nephritis. A reduction of the anionic charge was induced by lysozyme administration. The animals were divided into the following four groups: normal controls (group A), group B treated with lysozyme, group C treated with lysozyme and immunized with autologous tubular antigen, and group D immunized with the antigen. Proteinuria was marked in groups B, C and D as compared with group A, especially in group D. Development of Heymann nephritis by the tubular antigen was suppressed in group C. Stainings of anionic substances in the glomerular capillary walls by cationic probes were weakened in groups B, C and D. Immune complex deposited in the subepithelial area of the capillary wall was also stained by cationic probes. The findings suggest that reduced glomerular anionic charge by polycations results in suppressed immune complex deposition in the basement membrane, partially due to increased permeation of macromolecules across the glomerular capillary walls in rats.

Topics: Animals; Anions; Antigen-Antibody Complex; Basement Membrane; Capillary Permeability; Immunization; Kidney Glomerulus; Kidney Tubules; Microscopy, Electron; Muramidase; Nephritis; Rats; Rats, Inbred Strains; Staining and Labeling

Topics: Animals; Muramidase; Nephritis; Nucleosides; Rats

Topics: Humans; Muramidase; Nephritis

Topics: Animals; Arthritis; Arthus Reaction; Cell Adhesion; Cell Movement; Complement System Proteins; Connective Tissue; Cytoplasmic Granules; Glucuronidase; Humans; In Vitro Techniques; Inflammation; Microscopy, Electron; Muramidase; Nephritis; Neutrophils; Peroxidases; Phagocytosis; Rabbits

Topics: Acute Kidney Injury; Clinical Enzyme Tests; Diagnosis, Differential; Glomerulonephritis; Humans; Kidney Diseases; Kidney Tubules; Muramidase; Nephritis; Nephrosclerosis; Proteinuria

Topics: Acute Kidney Injury; Blood Urea Nitrogen; Glomerulonephritis; Humans; Kidney Diseases; Kidney Tubules; Leukemia; Muramidase; Nephritis; Nephrocalcinosis; Nephrotic Syndrome; Urinary Calculi

Topics: Child; Dermatologic Agents; Humans; Muramidase; Nephritis; Nephrotic Syndrome; Urine