muramidase and Nasopharyngeal-Neoplasms

To study the relationship between macrophages (MP), Langerhans cells (LC), T and B lymphocytes, biological behavior and prognosis of stage III nasopharyngeal carcinoma (NPC) patients.. 43 cases with stage III NPC were studied by immunohistochemical SP staining methods.. The positive expression rates of infiltrating LC and/or MP in NPC were 41%, 65% (CD68+), 46% (lysozyme+), and were related to prognosis, but not to age, sex, metastasis to cervical lymph nodes and basi-cranial destruction and/or cranial nerve damage. In addition, infiltrating T or B cells in NPC were not related to the prognosis or biological behavior. However, when both infiltrating T lymphocytes and LC and/or MP were marked, the prognosis of these cases were better than that of those cases whose infiltrating LC and/or MP were marked but T cell infiltration was scarce.. The prognosis of stage III NPC is related to LC or MP, survival is especially longer in those with marked T cell infiltration.

Topics: Adult; Aged; Antigens, CD; Antigens, Differentiation, Myelomonocytic; Female; Humans; Immunohistochemistry; Langerhans Cells; Lymphatic Metastasis; Lymphocytes, Tumor-Infiltrating; Macrophages; Male; Middle Aged; Muramidase; Nasopharyngeal Neoplasms; Neoplasm Staging; Prognosis; S100 Proteins; Survival Analysis

An unusual case of granulocytic sarcoma with a large retro-orbital tumor mass is described. The tumor had an uncommon cytomorphology and ultrastructure that mimicked a signet ring cell lymphoma. It was negative by chloroacetate esterase (CAE) stain. The patient was treated successfully with CHOP-regimen polychemotherapy and irradiation. Seventeen months after the initial diagnosis of malignant lymphoma, acute myeloid leukemia developed. Additional immunohistochemistry, including an immunoperoxidase staining for lysozyme, clearly demonstrated the early myeloid nature of the original tumor. This case emphasizes the importance of staining for lysozyme and other myeloid markers in addition to CAE staining in cases that demonstrate unusual morphological features.

Topics: Carboxylic Ester Hydrolases; Humans; Immunohistochemistry; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Male; Microscopy, Electron; Middle Aged; Muramidase; Nasopharyngeal Neoplasms

Topics: Adult; Histocytochemistry; Humans; Immunoenzyme Techniques; Macrophages; Middle Aged; Muramidase; Nasopharyngeal Neoplasms

A 36 year old woman presented with a nasopharyngeal tumour which was diagnosed and treated as diffuse large cell lymphoma. Twelve mth later the patient developed acute myeloid leukemia. At this stage, the original biopsies were reviewed and considered in retrospect to be granulocytic sarcoma on the basis of staining for chloracetate esterase and lysozyme. She achieved and maintained marrow and peripheral blood remission with chemotherapy, but developed several cutaneous nodules and 2 breast lumps. One breast lump was excised and was found, by the use of monoclonal antibodies, to carry myeloid markers. Thus monoclonal antibodies provided additional confirmatory evidence for the diagnosis of granulocytic sarcoma.

Topics: Adult; Antibodies, Monoclonal; Antineoplastic Combined Chemotherapy Protocols; Carboxylic Ester Hydrolases; Female; Humans; Leukemia, Myeloid; Muramidase; Nasopharyngeal Neoplasms

Forty-nine biopsy specimens of nasopharyngeal carcinoma and 20 biopsy specimens of nasopharyngeal mucosa obtained from non-cancer patients were studied for the presence of T-zone histiocytes (Langerhans' cells and their precursors) and macrophages by immunohistochemical methods with the use of antibodies against S-100 protein and lysozyme (lys), respectively. Patients with dense infiltration of T-zone histiocytes in the primary sites survived longer than those without such infiltration (mean survival times, 8, 39, and 72 months in the patients with the density of -, +, and ++, respectively; P less than 0.005). In contrast, there was no relationship between patient prognosis and density of lys+ macrophages in the tumor tissue (P = 0.33). The distribution of T-zone histiocytes in the tumor tissue was different from that of macrophages. T-zone histiocytes may play an important role in the immune reaction against cancer, probably acting through mechanism different from that of macrophages.

Topics: Adolescent; Adult; Aged; Child; Female; Histiocytes; Histocytochemistry; Humans; Macrophages; Male; Middle Aged; Muramidase; Nasopharyngeal Neoplasms; Prognosis; S100 Proteins