muramidase and Mast-Cell-Sarcoma

An extremely rare solitary mast cell tumor of the lung was studied histologically, immunohistochemically, and ultrastructurally. The histologic features of the tumor included nodular growth of well-differentiated mast cells and clear cells with no granules. The current case is the third case of a solitary mast cell tumor (granuloma) of the lung in the literature. Clinicopathologic features of this tumor are compared with the other two cases reported previously in the international literature, and the nature of the clear cells is discussed.

Topics: alpha 1-Antitrypsin; Antigens, Neoplasm; Apoproteins; Diagnosis, Differential; Granuloma; Humans; Keratins; Lung Neoplasms; Male; Mast Cells; Mast-Cell Sarcoma; Membrane Glycoproteins; Middle Aged; Mucin-1; Muramidase; Neoplasm Proteins; Pulmonary Surfactant-Associated Proteins; Pulmonary Surfactants

It was reported that neurotropin (NSP), an extract isolated from the inflamed skins of rabbits inoculated with vaccinia virus, activates murine T cell functions participating in cell-mediated immunity. The present study was undertaken to examine the effect of NSP on plastic dish-adherent macrophages (M phi) from ddY mice in vitro. Total activities of beta-glucuronidase and N-acetyl-beta-D-glucosaminidase in resident peritoneal M phi was slightly enhanced when the M phi were cultured with NSP (10-1000 micrograms/ml) for 48 and 96 hr, but no enhancement was noted in 24 hr culture. Intracellular activity of lactate dehydrogenase (LDH) was also strongly enhanced in a dose-dependent manner by culturing with NSP for 48 and 96 hr. The enhanced LDH activity in the M phi cultured with NSP for 96 hr was completely inhibited by cycloheximide, an inhibitor of protein synthesis. In addition, consumption of glucose in the culture media by the M phi was also enhanced by culturing with NSP for 96 hr. Intracellular activity of LDH and glucose consumption of plastic dish-nonadherent cells from normal mouse peritoneal cells, however, was not enhanced by NSP in 96 hr culture. In regard to allogeneic M phi-mediated cytostatic activity to P815-X2 mastocytoma, NSP had no effect on cytostatic activities of the resident and thioglycollate-induced M phi, although NSP by itself dose-dependently inhibited the growth of P815-X2 mastocytoma without affecting cell viability. These results suggest that NSP biochemically activates mouse peritoneal M phi in vitro, but the M phi activated by NSP can not inhibit the growth of P815-X2 mastocytoma.

Topics: Adjuvants, Immunologic; Animals; Antineoplastic Agents; Ascitic Fluid; Cell Line; Glucose; Macrophage Activation; Macrophages; Male; Mast-Cell Sarcoma; Mice; Muramidase; Polysaccharides; Thymidine

The lysozyme (muramidase) activity was measured in the sera of 84 dogs with neoplastic disease. Neoplasms included 32 lymphomas, 13 primary bone neoplasms, 5 melanomas, 5 thyroid neoplasms, 9 soft tissue sarcomas, 5 mast cell sarcomas, and 15 carcinomas. The sera from 21 healthy dogs served as control. Dogs with neoplastic disease had significantly (P less than 0.005) higher serum lysozyme activity than did the healthy controls. For lymphosarcoma, dogs with clinical signs of systemic disease had significantly higher serum lysozyme activity than did dogs without clinical signs. For bone neoplasms, dogs with metastatic disease had higher serum lysozyme activity than did dogs without metastasis. Increased lysozyme activity may be a useful marker of macrophage-mediated host responses to neoplasms in dogs.

Topics: Animals; Bone Neoplasms; Dog Diseases; Dogs; Lymphoma; Mast-Cell Sarcoma; Muramidase; Neoplasms; Sarcoma; Thyroid Neoplasms

Sodium thioglycollate-induced peritoneal macrophages from C3D2F1 mice were examined for their ability to produce and secrete lysozyme in vitro. Lysozyme was quantitated by use of a "lysoplate assay", in which lyophilized Micrococcus lysodeicticus suspended in agar were lysed by the enzyme. The ability to secrete lysozyme decreased in mice bearing a subcutaneous or intraperitoneal mastocytoma P-815 with progressive tumour growth.

Topics: Animals; Ascitic Fluid; Cells, Cultured; Macrophages; Mast-Cell Sarcoma; Mice; Muramidase; Neoplasm Transplantation; Sarcoma, Experimental; Thioglycolates

Topics: Animals; Carbon Isotopes; Cold Temperature; Drug Stability; Mast-Cell Sarcoma; Metabolism; Mice; Muramidase; Ovalbumin; Proteins; Serine; Stimulation, Chemical; Time Factors; Xylose