muramidase and Mandibular-Neoplasms

A Bernese mountain dog was evaluated because of a gingival mass, multiple abdominal masses, and a pulmonary mass. Malignant histiocytosis was diagnosed based on cytological examination of splenic and bone marrow aspirates and histological examination of a bone marrow biopsy and the gingival mass. The case demonstrates that malignant histiocytosis is difficult to diagnose due to the variety of histiocytic disorders.

Topics: alpha 1-Antitrypsin; Animals; Bone Marrow; Diagnosis, Differential; Dog Diseases; Dogs; Gingiva; Histiocytic Sarcoma; Male; Mandibular Neoplasms; Muramidase; Spleen

A mandibular eosinophilic granuloma in a 16-year-old male is reported. This case showed rapid regression, which was clearly demonstrated by histopathological examinations of both preoperative biopsy and surgical materials. Transformation from an eosinophilic granuloma to a xanthomatous granuloma with multinucleated giant cells was observed after only 26 days. Special staining of paraffin sections with peanut agglutinin (PNA) and use of electron microscopy showed that the main component of the lesion in the biopsy material was Langerhans-type histiocytes. These cells had disappeared from the lesion by the time of the operation. At the same time, the number of infiltrating eosinophils was also markedly reduced. It seems appropriate to consider that the rapid regression of this disease was correlated with the rapid reduction in the number of Langerhans-type histiocytes appearing in the granulomatous foci, as well as the number of infiltrating eosinophils.

Topics: Adolescent; alpha 1-Antichymotrypsin; Eosinophilic Granuloma; Histiocytes; Humans; Immunohistochemistry; Langerhans Cells; Lectins; Male; Mandibular Neoplasms; Microscopy, Electron; Muramidase; Peanut Agglutinin; S100 Proteins; Staining and Labeling