muramidase and Lymphoproliferative-Disorders

Topics: Hexosaminidases; Humans; Isoenzymes; Karyotyping; Leukemia; Lymphocytes; Lymphoma; Lymphoproliferative Disorders; Muramidase; Nucleotidyltransferases; Plasmacytoma; Prognosis; Waldenstrom Macroglobulinemia

Immunoreactive lysozyme was readily detectable in canine histiocytic disorders including systemic histiocytosis, malignant histiocytosis and granulomatous panniculitis. Lysozyme was less reliable as a histiocytic marker in cutaneous histiocytoma; forty percent of these tumors were negative for lysozyme expression. The marked heterogeneity in lysozyme expression in cutaneous histiocytoma may indicate that a proportion of these tumors show relatively primitive histiocytic differentiation and do not express lysozyme. Alternatively, this same proportion may exhibit a phenotype akin to cutaneous Langerhans cells which do not contain lysozyme. Lysozyme was not detectable in the tumor cells in lymphomatoid granulomatosis, atypical cutaneous histiocytoma, and histiocytic lymphosarcoma. Other evidence that these three disorders do not represent true histiocytic proliferative disorders is discussed.

Topics: Animals; Cytoplasm; Dog Diseases; Dogs; Histiocytes; Histiocytic Sarcoma; Histiocytoma, Benign Fibrous; Lymphoma, Large B-Cell, Diffuse; Lymphomatoid Granulomatosis; Lymphoproliferative Disorders; Muramidase

Intracytoplasmic lysozyme was studied by the peroxidase antiperoxidase (PAP) and protein A-peroxidase methods in 130 cases of various myeloproliferative and lymphoproliferative disorders and 21 lymph nodes and bone marrow metastases from solid primary tumors. This marker, which can be identified in formalin or Zenker-fixed tissues, as well as in peripheral blood and bone marrow smears, proved useful to distinguish malignant myeloid and histiocytic tumors from malignant lymphoid and undifferentiated epithelial metastases. The diagnostic application of these findings are discussed.

Topics: Cytoplasm; Humans; Immunoenzyme Techniques; Leukemia; Lymphoma; Lymphoproliferative Disorders; Monocytes; Muramidase; Myeloproliferative Disorders

Topics: Granuloma; Granulomatosis with Polyangiitis; Granulomatous Disease, Chronic; Humans; Lymphomatoid Granulomatosis; Lymphoproliferative Disorders; Muramidase; Peptidyl-Dipeptidase A; Sarcoidosis

Lysozyme activity was determined in the serum, urine and leukocytes of 53 patients with immunocytoma and 24 patients with lymphoproliferative syndromes without associated monoclonal gammapathy. In patients with multiple myeloma the frequency of low serum lysozyme activity and high leukocyte lysozyme activity was higher. In the cases with renal failure, lysozyme activity was raised in serum and urine, and the 24-hour urinary lysozyme excretion was increased. In 7 patients with increased urinary lysozyme excretion no clinical or laboratory evidence of renal complications was found. Relative monocytosis in peripheral blood was observed in half of the cases of multiple myeloma, and in these patients also in about half of the cases the lysozyme activity was raised in the leukocytes and urine, and the 24-hour urinary lysozyme excretion was increased. In patients with Hodgkin's disease, lymphosarcoma and chronic lymphatic leukemia the frequency of low serum lysozyme activity was increased.

Topics: Adult; Aged; Humans; Kidney Diseases; Kidney Failure, Chronic; Leukocytes; Lymphoproliferative Disorders; Middle Aged; Multiple Myeloma; Muramidase; Waldenstrom Macroglobulinemia