muramidase and Laryngeal-Neoplasms

The purpose of this study was to discuss the anti-tumor function of lysozyme (LZM) to laryngocarcinoma. The LZM distribution of regional lymph nodes incised from laryngocarcinoma patients in paraffin sections was observed by immunohistochemical technique. By the cell culture technique, autologus laryngocarcinoma cells were used as target cells to evaluate the tumoridical activity of MØ which were isolated from the regional lymph nodes of laryngocarcinoma patients. At the same time, ConA was added as control group. The supernatant of cell culture was collected after 24 hours to detect LZM content. The results indicated that the number of LPM in non-metastatic lymph nodes was significantly more than in metastatic lymph nodes (P < 0.01). The MØ isolated from patients' regional lymph nodes released LZM content and it's tumoridical activity to autologus tumor cells were significantly increased after adding ConA (P < 0.01). The LZM releasing content and tumoridical activity of MØ in non-metastatic lymph nodes were much more than metastatic lymph nodes (P < 0.05). The LZM content and tumoridical activity of MØ were analyzed with linearity relapsing statistics, it was positive linearity relation (r = 0.94, P < 0.01). All above suggests that the LZM of laryngocarcinoma patients is closely related to the anti-tumor function, it improves the tumoridical activity of MØ to autologus laryngocarcinoma cells and accelerates the death of laryngocarcinoma cells.

Topics: Adult; Aged; Carcinoma, Squamous Cell; Female; Humans; Laryngeal Neoplasms; Lymph Nodes; Macrophages; Male; Middle Aged; Muramidase; Neck; Tumor Cells, Cultured

The A. inform about the results achieved with lysozyme chlorhydrate in the treatment of 15 patients with juvenile laryngeal papillomatosis. The lysozyme is an electropositive enzyme which synthesis is related to the degree of proteins and vitamin B complex ingestion. Lysozyme is a component of the immunitary inespecific system, serving to prevent against HPV-DNA at the level of the secretory film of the mucociliary apparatus of the respiratory mucous membrane. Furthermore, lysozyme hydrolyzes the mucopolysaccharide of the connective tissue and inhibits the virus-DNA replication. 100-300 mgr daily during 30-60 days simultaneously with hyperproteic diet and vitamin B complex (after correction of the nutrimental deficiencies) brought about the evanishment of papillomatosis. The A. suggest that the predisposition to infection by virus DNA is primarily of immunitary origin, because of lysozyme deficiency, and secondary due to a low intake of proteins and vitamin B complex.

Topics: Administration, Oral; Adolescent; Adult; Child; Child, Preschool; Drug Evaluation; Humans; Laryngeal Neoplasms; Middle Aged; Muramidase; Papilloma

Gelatinous and telangiectatic types can be differentiated among the human vocal fold polyps. Telangiectatic polyps are characterized by eosinophilic deposits consisting of fibrin and cellular blood constituents. Labyrinthine vascular channels are characteristic of these polyps, and are partially or completely lined by a single layer of flat cells. Using electron microscopy and immunohistochemical stainings (antibodies against factor VIII-related antigen, Ulex europaeus I lectin, and antibodies against lysozyme), we found that the lining cells are true vascular endothelial cells and are not organizing histiocytic cells that are arranged in an endothelial-like pattern.

Topics: Antigens; Factor VIII; Humans; Laryngeal Neoplasms; Microscopy, Electron; Muramidase; Polyps; von Willebrand Factor

Topics: Female; Humans; Laryngeal Neoplasms; Male; Muramidase

Topics: Adolescent; Child; Child, Preschool; Estrogens, Conjugated (USP); Female; Humans; Immunotherapy; Laryngeal Neoplasms; Male; Muramidase; Papilloma

Topics: Amnion; Animals; Antineoplastic Agents; Carcinoma, Squamous Cell; Cell Line; HeLa Cells; Humans; Laryngeal Neoplasms; Mathematics; Mitosis; Muramidase; Ovalbumin; Placenta

Topics: Animals; Bone Marrow; Bone Marrow Cells; Carcinoma; Cell Line; Chick Embryo; Culture Techniques; Embryo, Mammalian; Fibroblasts; Haplorhini; HeLa Cells; Histiocytes; Humans; Kidney; L Cells; Laryngeal Neoplasms; Leukocytes; Lung; Macrophages; Mice; Muramidase; Muscles; Rats; Skin

Topics: Antiviral Agents; Humans; Laryngeal Neoplasms; Larynx; Mucus; Muramidase; Nasal Cavity; Paranasal Sinuses