muramidase and Hepatoblastoma

muramidase has been researched along with Hepatoblastoma* in 2 studies

Other Studies

2 other study(ies) available for muramidase and Hepatoblastoma

Article	Year
Dodging cellular customs: smuggling macromolecules into hepatocytes. Hepatology (Baltimore, Md.), 1994, Volume: 20, Issue:6 Topics: Asialoglycoproteins; Hepatoblastoma; HN Protein; Humans; Liver; Liver Neoplasms; Macromolecular Substances; Membrane Fusion; Microinjections; Muramidase; Parainfluenza Virus 1, Human; Receptors, Cell Surface; Tumor Cells, Cultured; Viral Fusion Proteins	1994
Fusion-mediated microinjection of lysozyme into HepG2 cells through hemagglutinin neuraminidase-depleted Sendai virus envelopes. The Journal of biological chemistry, 1994, Jan-21, Volume: 269, Issue:3 The potential of reconstituted Sendai viral envelopes containing only the fusion protein (F-virosomes) was evaluated for a targeted cytosolic delivery of lysozyme to human hepatoblastoma cells (HepG2) in culture. 125I-Lysozyme loaded into F-virosomes was used to monitor its fusion-mediated transfer to the HepG2 cells. Using fusion assay based on the transfer of water soluble probe, we have demonstrated the existence of aqueous connection between F-virosomes and target cells. Target specificity of the F-virosomes was ensured by the strong interaction between terminal beta-galactose moiety of F protein and the asialoglycoprotein receptor on the membrane of HepG2 cells. Incubation of the loaded F-virosomes with cells resulted in fusion-mediated injection, as inferred from the ability of cells to internalize lysozyme in the presence of azide (an inhibitor of the endocytotic process). Binding as well as fusion of the F-virosomes to HepG2 cells was solely mediated by the F protein. Introduction of 125I-lysozyme into the HepG2 cells was confirmed by selective accumulation of acid and antibody-precipitable radioactivity in the cytosolic compartment. The structural integrity of the internalized lysozyme was also assessed. The potential usefulness of F-virosomes with defined specificities as biological carrier for both in vitro and in vivo cytosolic delivery of macromolecules and drugs has been established. Topics: Asialoglycoprotein Receptor; Asialoglycoproteins; Biological Transport; Cell Line; Galactose; Hepatoblastoma; Humans; Kinetics; Liver Neoplasms; Membrane Fusion; Microinjections; Muramidase; Neuraminidase; Parainfluenza Virus 1, Human; Receptors, Cell Surface; Subcellular Fractions; Tumor Cells, Cultured; Viral Fusion Proteins	1994

Article

Year

Dodging cellular customs: smuggling macromolecules into hepatocytes.

Hepatology (Baltimore, Md.), 1994, Volume: 20, Issue:6

Topics: Asialoglycoproteins; Hepatoblastoma; HN Protein; Humans; Liver; Liver Neoplasms; Macromolecular Substances; Membrane Fusion; Microinjections; Muramidase; Parainfluenza Virus 1, Human; Receptors, Cell Surface; Tumor Cells, Cultured; Viral Fusion Proteins

1994

Fusion-mediated microinjection of lysozyme into HepG2 cells through hemagglutinin neuraminidase-depleted Sendai virus envelopes.

The Journal of biological chemistry, 1994, Jan-21, Volume: 269, Issue:3

The potential of reconstituted Sendai viral envelopes containing only the fusion protein (F-virosomes) was evaluated for a targeted cytosolic delivery of lysozyme to human hepatoblastoma cells (HepG2) in culture. 125I-Lysozyme loaded into F-virosomes was used to monitor its fusion-mediated transfer to the HepG2 cells. Using fusion assay based on the transfer of water soluble probe, we have demonstrated the existence of aqueous connection between F-virosomes and target cells. Target specificity of the F-virosomes was ensured by the strong interaction between terminal beta-galactose moiety of F protein and the asialoglycoprotein receptor on the membrane of HepG2 cells. Incubation of the loaded F-virosomes with cells resulted in fusion-mediated injection, as inferred from the ability of cells to internalize lysozyme in the presence of azide (an inhibitor of the endocytotic process). Binding as well as fusion of the F-virosomes to HepG2 cells was solely mediated by the F protein. Introduction of 125I-lysozyme into the HepG2 cells was confirmed by selective accumulation of acid and antibody-precipitable radioactivity in the cytosolic compartment. The structural integrity of the internalized lysozyme was also assessed. The potential usefulness of F-virosomes with defined specificities as biological carrier for both in vitro and in vivo cytosolic delivery of macromolecules and drugs has been established.

Topics: Asialoglycoprotein Receptor; Asialoglycoproteins; Biological Transport; Cell Line; Galactose; Hepatoblastoma; Humans; Kinetics; Liver Neoplasms; Membrane Fusion; Microinjections; Muramidase; Neuraminidase; Parainfluenza Virus 1, Human; Receptors, Cell Surface; Subcellular Fractions; Tumor Cells, Cultured; Viral Fusion Proteins

1994