muramidase and Eosinophilic-Granuloma

Immunohistochemical phenotyping of 7 cases of histioeosinophilic granulomas of thymus and 4 cases of reactive eosinophilic pleuritis was performed. All 11 cases of these 2 entities reacted identically. This supports the view that these 2 lesions are similar in nature. Both lesions are reactions to the presence of insufflated gas and its resorption.

Topics: Antibodies, Monoclonal; Biomarkers; Eosinophilic Granuloma; Humans; Immunohistochemistry; Lymphatic Diseases; Muramidase; Myasthenia Gravis; Phenotype; Pleurisy; Receptors, Cholinergic; Thymus Gland

A mandibular eosinophilic granuloma in a 16-year-old male is reported. This case showed rapid regression, which was clearly demonstrated by histopathological examinations of both preoperative biopsy and surgical materials. Transformation from an eosinophilic granuloma to a xanthomatous granuloma with multinucleated giant cells was observed after only 26 days. Special staining of paraffin sections with peanut agglutinin (PNA) and use of electron microscopy showed that the main component of the lesion in the biopsy material was Langerhans-type histiocytes. These cells had disappeared from the lesion by the time of the operation. At the same time, the number of infiltrating eosinophils was also markedly reduced. It seems appropriate to consider that the rapid regression of this disease was correlated with the rapid reduction in the number of Langerhans-type histiocytes appearing in the granulomatous foci, as well as the number of infiltrating eosinophils.

Topics: Adolescent; alpha 1-Antichymotrypsin; Eosinophilic Granuloma; Histiocytes; Humans; Immunohistochemistry; Langerhans Cells; Lectins; Male; Mandibular Neoplasms; Microscopy, Electron; Muramidase; Peanut Agglutinin; S100 Proteins; Staining and Labeling

S-100 protein immunostaining has been advocated to identify the characteristic Langerhans' cells in the histologic diagnosis of PEG. Reliable demonstration of an increased number of Langerhans' cells is essential in difficult biopsy cases, since occasional Langerhans' cells can be found in other pulmonary lesions. We examined the S-100 protein labeling pattern in three cases of PEG and in a variety of controls. Non-Langerhans' histiocytes were labeled for lysozyme antigen on the same histologic sections using a combined ABC and PAP technique. This verified that the S-100 protein-negative histiocytes were indeed a separate population from the S-100 protein-positive histiocytes and did not represent Langerhans' cells which failed to label with antiserum to S-100 protein. This technique confirms the usefulness of S-100 protein staining in the diagnosis of PEG and offers a means to verify the reliability of the S-100 protein labeling in questionable cases.

Topics: Biopsy; Eosinophilic Granuloma; Granulocytes; Histiocytes; Humans; Immunohistochemistry; Langerhans Cells; Lung; Lung Diseases; Muramidase; S100 Proteins; Staining and Labeling

Immunohistochemical examinations were performed using five kinds of histiocytic markers [S100 protein, lysozyme, non-specific cross reacting antigen with carcinoembryonic antigen (NCA), alpha 1-antichymotrypsin (alpha 1-ACT) and alpha 1-antitrypsin (alpha 1-AT)] in biopsied tissues from histiocytosis X, juvenile xanthogranuloma, xanthoma tuberosum, xanthoma disseminatum, reticulohistiocytic granuloma and multicentric reticulohistiocytoma, all of which have been classified as histiocytic proliferative disorders. Our results suggested that xanthomatous lesions of the skin to be composed of the histiocytic proliferation of two different cell lineages, i.e. S100+lyso-NCA- T-zone histiocytes and S100-lyso+NCA+ tissue macrophages. Only lesions of histiocytosis X were composed of the former cells. It is suggested that these markers will be useful in determining the delineation of the histiocytic system on the basis of functional heterogeneity.

Topics: alpha 1-Antichymotrypsin; alpha 1-Antitrypsin; Biopsy; Bone and Bones; Carcinoembryonic Antigen; Eosinophilic Granuloma; Histiocytoma, Benign Fibrous; Histiocytosis, Langerhans-Cell; Humans; Immunohistochemistry; Lymphatic Diseases; Muramidase; S100 Proteins; Skin; Skin Neoplasms; Xanthogranuloma, Juvenile