muramidase and Endometriosis

The diagnosis of the internal endometriosis was verified in histopathological investigation in 16 patients. The following indices were evaluated in this group: 1) the ability of reduction of nitroblue tetrazolium (NBT) by neutrophils with the calculation of indices of spontaneous and stimulating reduction by latex of this dye, 2) activity of myeloperoxidase (MPO) in neutrophils with the semiquantitative measurement determined in blood smears, 3) activity of alkaline phosphatase (FA) in neutrophils with the semiquantitative measurement determined in blood smears, 4) lysozyme activity in blood serum by turbidimetric method. The obtained results were compared with control values obtained in 20 healthy women. The following statistically significant differences were shown-the increase of spontaneous and stimulated reduction of NBT, the decrease of myeloperoxidase activity, the increase of alkaline phosphatase activity and the increase of the activity of lysozyme in diluted and undiluted blood serum. On this basis the following conclusion is suggested: The neutrophils of the peripheral blood in patients with internal endometriosis are characterized by metabolic disturbances and the change of the function of cell structures. The indices of these changes are myeloperoxidase and alkaline phosphatase. The above changes may have an important influence on defensive function of these cells in the case of real infectious conditions.

Topics: Adult; Alkaline Phosphatase; Endometriosis; Female; Humans; Middle Aged; Muramidase; Neutrophils; Peroxidase

Persistent mating-induced endometritis (PMIE) severely decreases fertility in horses. The aim of the present study was to evaluate differences between horses susceptible to PMIE and a control group in terms of the expression of selected immune response and effector genes, and the effects of oestrous cycle stage on this expression. Endometrial biopsies from 18 uterine samples of mares in the control group (eight in dioestrus, 10 in oestrus) and 16 PMIE-susceptible mares (four in dioestrus, 12 in oestrus) were analysed by quantitative real-time reverse transcription-polymerase chain reaction. Genes for pathogen recognition receptors Toll-like receptor 2 (TLR2) and NLR family CARD domain containing 5 (NLRC5), as well as tissue-specific inhibitor of metalloproteinase 1 (TIMP1), C-X-C motif chemokine ligand (CXCL) 9, CXCL10 and CXCL11 and uteroferrin were expressed at similar levels in the control group and in susceptible mares. Genes for C-C motif chemokine ligand 2 (CCL2) and the antimicrobial peptides secreted phospholipase A2 (sPLA2), lipocalin 2 and lactoferrin were all expressed at higher levels in susceptible compared with control mares. The expression of genes for the antimicrobial peptides equine β-defensin 1 (EBD1), lysozyme (LYZ) and secretory leukoprotease inhibitor (SLPI) was also higher in susceptible than control mares. The diagnostic sensitivity of assays for EBD1, LYZ and SLP1 gene expression to detect susceptibility to PMIE was estimated to be 100%, 94% and 100% respectively, with specificities of 83%, 78% and 78% respectively. When all three tests were positive, the specificity increased to 94%, with an overall sensitivity of 94%. The present study has yielded insights into pathophysiological changes in mares susceptible to PMIE and identified robust diagnostic markers (EBD1, LYZ and SLPI) for susceptibility to this disease.

Topics: Animals; Collagen Type VII; Endometriosis; Endometrium; Estrous Cycle; Female; Fertility; Gene Expression Regulation; Horses; Immunity, Innate; Male; Muramidase; Reproduction; RNA, Messenger; Secretory Leukocyte Peptidase Inhibitor; Sexual Behavior, Animal

An intraperitoneal inflammatory process has been associated with infertility in women without anatomic distortion of the pelvic viscera, particularly with endometriosis. This phenomenon was investigated by measuring peritoneal fluid (PF) and serum levels of a major secretory product of the macrophage, lysozyme, in fertile and infertile women undergoing laparoscopy. Serum lysozyme levels were in the normal range and did not correlate with total PF lysozyme. Regression analysis revealed a negative correlation between adnexal adhesions and PF volume (P less than 0.05), PF leukocyte number (P less than 0.05), and total PF lysozyme (P less than 0.05). Significant correlations were found between total PF lysozyme and PF volume (r = 0.72, P less than 0.0001) and leukocyte number (r = 0.67, P less than 0.0001). These results suggest that a localized intraperitoneal inflammatory reaction is associated with infertility in the absence of anatomic distortion of the pelvic viscera.

Topics: Adnexal Diseases; Ascitic Fluid; Endometriosis; Female; Humans; Infertility, Female; Leukocyte Count; Muramidase; Tissue Adhesions