muramidase and Eczema

Topics: Anaphylaxis; Child; Child, Preschool; Eczema; Humans; Infant, Newborn; Muramidase; Skin Tests

In vitro degranulation of polymorphonuclear leukocytes, which were stimulated either with synthetic chemotactic peptide (N-formyl-methionyl-leucyl-phenylalanine, FMLP) or with C3b-opsonized zymosan as a promotor of phagocytosis, was studied in 66 patients with psoriasis, 18 lesion-free psoriatics, 18 healthy subjects, and 14 other dermatological disorder controls. Stimulated release of lysozyme (from specific granules and azurophil granules) and beta-glucuronidase (from azurophil granules) in the presence of both FMLP and serum-activated zymosan was markedly reduced in patients with actively spreading guttate psoriatic lesions, in whom relapse of lesions lasted for less than 1 month and papules involved about 13-25% of skin surface. In contrast, stimulated degranulation was within normal range in active plaque psoriasis, stationary plaque psoriasis, symptomless psoriatics, and patients with disseminated eczema. Spontaneous release of lysozyme and beta-glucuronidase (background) was found to be not different in all groups studied; however, patients with active guttate psoriasis had significantly lower total lysozyme activity than those with active and stationary plaque psoriasis as well as psoriatics in the remission. These data are in favor of in vivo activation of neutrophils in active guttate psoriasis by some factors related to the early relapse of the lesions. This results in a possible combination of the following phenomena: (1) in vivo partial degranulation of neutrophils; (2) induction of "unresponsiveness state" of these cells to subsequent in vitro stimulation; and/or (3) migration of highly responsive neutrophils to skin lesions, which leaves in the circulation the subpopulation less reactive to chemotactic and phagocytic stimuli.

Topics: Cells, Cultured; Chemotaxis, Leukocyte; Complement C3; Cytoplasmic Granules; Eczema; Glucuronidase; Humans; Muramidase; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Photosensitivity Disorders; Psoriasis; Zymosan

Topics: Adolescent; Child; Child, Preschool; Complement System Proteins; Eczema; Hemagglutination Tests; Humans; Immunity, Innate; Infant; Isomerism; Lysine; Muramidase; Neurodermatitis; Neutrophils

Topics: Adolescent; Age Factors; Child; Child, Preschool; Eczema; Humans; Immunity, Active; Infant; Muramidase; Neurodermatitis; Phagocytosis

Topics: Adolescent; Adrenal Cortex Hormones; Adult; Aged; Blood Bactericidal Activity; Chronic Disease; Complement System Proteins; Eczema; Female; Humans; Immunity; Male; Middle Aged; Muramidase; Time Factors

Topics: Acute Disease; Adult; Aged; Blood Bactericidal Activity; Chronic Disease; Complement System Proteins; Eczema; Female; Humans; Male; Muramidase

Topics: Child; Child, Preschool; Complement System Proteins; Eczema; Hemagglutination Tests; Humans; Immunoglobulins; Muramidase; Neurodermatitis; Phagocytosis

Topics: Adolescent; Child; Child, Preschool; Complement System Proteins; Eczema; Hemagglutination Tests; Humans; Immunoglobulins; Infant; Muramidase; Neurodermatitis; Phagocytosis

Topics: Eczema; Gangrene; Granulation Tissue; Humans; Leg Ulcer; Muramidase; Peripheral Vascular Diseases; Pharmacology; Phlebitis; Varicose Ulcer; Vascular Diseases