muramidase and Duodenitis

The article presents findings allowing estimating effect of local application of polioxidonium and yantum verde in 101 children aged 12-17 with chronic catarrhal gingivitis and chronic gastroduodenitis. Statistically significant PMA indeх decrease (40.1±2.3% till 1.4±0.6% (р<0,001)) proved the above mentioned therapy scheme to be highly effective for treatment of chronic catarrhal gingivitis in children with chronic gastroduodenitis.

Topics: Adolescent; Anti-Inflammatory Agents, Non-Steroidal; Benzydamine; Child; Chronic Disease; Drug Therapy, Combination; Duodenitis; Female; Gastritis; Gingivitis; Humans; Immunoglobulin A; Immunoglobulin A, Secretory; Immunoglobulin G; Immunologic Factors; Interleukin-10; Interleukin-1beta; Male; Mouth; Muramidase; Piperazines; Polymers; Saliva

Lysozyme is as an innate enzyme with potent antibacterial properties found in Paneth cells in normal duodenal crypts. Since celiac disease concurs with an abnormal duodenal microbiota we explored the expression of lysozyme in this disease. Fifty-three duodenal biopsies were stained with anti-lysozyme: 15 had normal duodenal mucosa (NDM), 7 chronic active duodenitis (CAD), 3 borderline (BL), 17 subtotal villous atrophy (SVA) and 11 total villous atrophy (TVA). NDM showed lysozyme-positive Paneth cells arranged in "Indian file" in 93.3%. In contrast, lysozyme-positive mucus metaplasia in crypts (LPMMC) replacing Paneth cells was found in 71.5% in CAD, in 96.4% in SVA/TVA, and in 2 cases with B. In 19.3% cases with BL/SVA/TVA, LPMMC replaced all Paneth cells in all crypts in entire sections. In crypts and villi, lysozyme-positive goblet cells (LPGC) were found in 92.8%. Changes were more frequent in the duodenal bulb than in pars descendens. In normal duodenal mucosa, absorptive enterocytes and goblet cells migrate from stem cells upwards, while Paneth cells migrate downwards, towards the base of the crypts. In celiac disease stem cells seem to have been re-programmed, as the normal production of Paneth cells in the crypts was replaced by lysozyme-producing mucus cells. LPMMC and LPGC in celiac disease might mirror an antimicrobial adaptation of stem cells to signals generated by pathogenic duodenal bacteria. The molecular mechanism(s) behind the abrogation of Paneth cells in duodenal crypts and its substitution by LPMMC in celiac disease remains to be elucidated.

Topics: Celiac Disease; Child; Child, Preschool; Chronic Disease; Duodenitis; Duodenum; Female; Goblet Cells; Humans; Intestinal Mucosa; Male; Metaplasia; Microvilli; Muramidase; Paneth Cells; Staining and Labeling

To investigate aggressive-protective factors in patients with erosive lesions of gastroduodenal mucosa.. Intragastric pH-metry, tests for Helicobacter pylori, lysozyme activity, content of bile acid in gastric juice, components of gastric mucous gel were made in 106 patients.. It is shown that there are two alternative paths of erosions development in the gastroduodenal zone: infectious and non-infectious.. In patients infected with Helicobacter pylori, lowering of a protective ability of the mucous gel is caused by prevalence of synthesis of immature mucous components while in non-infected patients this happens as a result of high catabolism of mucous components.

Topics: Adult; Bile Acids and Salts; Duodenal Ulcer; Duodenitis; Gastric Juice; Gastric Mucosa; Gastritis; Helicobacter pylori; Humans; Hydrogen-Ion Concentration; Intestinal Mucosa; Middle Aged; Mucus; Muramidase; Peptic Ulcer; Stomach Ulcer

The study was concerned with measurement of lysozyme activity of saliva in healthy subjects and patients with different pathologies of the stomach including precancer and cancer. It established a considerable decrease in this parameter in cancer and precancer as compared with healthy controls. Assay of saliva lysozyme activity was found to yield more specific and prognostic data than clinical symptoms and examination of the patient. This procedure proved highly valuable in forming groups at risk for stomach cancer, due to providing significant differences in saliva lysozyme activity indices between "healthy subjects--stomach pathology" and "precancer--precancerous changes--stomach cancer" groups.

Topics: Clinical Enzyme Tests; Duodenal Ulcer; Duodenitis; Gastritis, Atrophic; Gastritis, Hypertrophic; Humans; Muramidase; Nephelometry and Turbidimetry; Precancerous Conditions; Prognosis; Risk Factors; Saliva; Sensitivity and Specificity; Stomach Neoplasms; Stomach Ulcer

The authors describe the time-course of changes in local and general immunity as influenced by multimodality treatment in different groups of patients suffering from CGD and concomitant pancreatoduodenal pathology. It has been found that associated microbial and inflammatory process in the biliary system exerts a pronounced effect on the changes in immunologic parameters and that antibacterial therapy produces a beneficial action on the normalization of the parameters under consideration.

Topics: Adolescent; Child; Chronic Disease; Combined Modality Therapy; Duodenitis; Gastroenteritis; Humans; Immunoglobulins; Muramidase

Topics: Adolescent; Biliary Dyskinesia; Child; Child, Preschool; Duodenitis; Gastric Juice; Gastritis; Humans; Immunoglobulins; Muramidase; Oropharynx; Pancreatitis; Saliva

The distribution of ferritin and lysozyme in 19 normal and abnormal duodenal biopsies was studied by an immunoperoxidase technique. The abnormal biopsies included cases of chronic duodenitis with gastric metaplasia, gastric heterotopia, villous atrophy, and a case of hemochromatosis. Ferritin is demonstrated in duodenal absorptive cells, with the staining being most intense in the hemochromatosis case. It was absent in duodenal cells showing gastric metaplasia and in the surface epithelial cells of most biopsies with villous atrophy and gastric heterotopia. Lysozyme-positive mononuclear inflammatory cells were markedly increased in all abnormal biopsies. Not all lysozyme-positive cells were ferritin positive. The latter were especially abundant in areas with gastric metaplasia. It is suggested that this abundance may be related to passive diffusion of intestinal contents, particularly iron, through the metaplastic areas, and consequently there may be a relationship between the presence of duodenal gastric metaplasia and uncontrolled iron absorption.

Topics: Atrophy; Biopsy; Choristoma; Duodenitis; Duodenum; Ferritins; Hemochromatosis; Humans; Immunoenzyme Techniques; Intestinal Mucosa; Metaplasia; Muramidase; Stomach Diseases

Topics: Adolescent; Antibody Formation; Child; Chronic Disease; Duodenitis; Gastric Juice; Gastritis; Humans; Immunity, Innate; Mucins; Muramidase

Topics: Child; Child, Preschool; Chronic Disease; Duodenitis; Duodenum; Female; Gastritis; Humans; Immunoglobulins; Intestinal Secretions; Male; Muramidase

In 158 patients with symptoms of non ulcerative dyspepsia, endoscopic, histologic and immunohystochemical studies were performed over three years with the aim of investigating the immunological involvement in the Chronic non specific Duodenitis (CND). After excluding 112 patients with associated pathologies of those not fulfilling technics requisites, a population of 46 subjects was selected and subsequently it was subdivided into four groups. A group of 15 patients with histologically normal duodeno served as control; the rest (31 patients) were grouped according to duodenal compromise into three groups of minor-major severity; Grade 1 (n=12); Grade 2 (n=12); Grade 3 (n=7). The number of immunoglobuline producing cells by mn2 of mucosal area and semiquantitative evaluation of mononucleated cells with cytoplasmatic activity to hydrolitic enzymes (lysozyme or muramidase) were compared among the 4 sub-groups. It was observed a variation in the immunoglobulines rate among controls (IgA: M: G=82, 52: 11, 01: 6,45) regarding the most severe degree (61, 56: 27,30: 11,14). The increase of IgMy G compared with controls was highly significant (p is less than 0.001) and it was noted a correlative IgA diminution. The contribution of hydrolitic enzymes through the presence of "activated" histiocytes was maximal in the most degree-coinciding with the increase of IgM and IgG. This findings indicated that a marked local activation of the Immunitary System type B is observed in the Chronic Nonspecific Duodenitis and its increment, expressed as quali-quantitative variations of the different immunologlobulines and muramidase producing cells, should have lythic activity in the highest degrees. We conclude, proposing this disease as a model of immunological aggression which affects bulbar mucosae.

Topics: Adult; Aged; Duodenitis; Duodenoscopy; Duodenum; Female; Humans; Immunoenzyme Techniques; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Immunoglobulins; Male; Middle Aged; Muramidase