muramidase and DNA-Virus-Infections

Lysozyme is well-known as an immune effector in the immune system. Here we identified three genes including one c-type lysozyme, Btlysc, and two i-type lysozymes, Btlysi1 and Btlysi2, from the whitefly Bemisia tabaci. All three lysozymes were constitutively expressed in different tissues and developmental stages, but the two types of lysozymes showed different expression patterns. The expression levels of Btlysi1 and Btlysi2 were dramatically induced after the whitefly fed with different host plants while the expression level of Btlysc kept unchanged. After fungal infection and begomovirus acquisition, Btlysc expression was significantly upregulated while Btlysi1 and Btlysi2 expression were basically not induced. Furthermore, we found that Btlysc showed muramidase and antibacterial activities. Altogether, our results suggest that the two types of lysozymes act in two different ways in B. tabaci, that is, Btlysc is involved in the whitefly immune system while Btlysi1 and Btlysi2 may play a role in digestion or nutrition absorption.

Topics: Animals; Beauveria; Begomovirus; Cloning, Molecular; DNA Virus Infections; Gene Expression Regulation; Gossypium; Hemiptera; Immunity, Innate; Insect Proteins; Muramidase; Mycoses; Pest Control; Protein Isoforms; Transcriptome

We report the effect of aqueous, ethanol, and methanol solvent leaf extracts of Punica granatum on innate immune mechanisms, such as phagocytosis activity, respiratory burst activity, alternative complement activity, lysozyme activity and functional immunity in terms of percentage cumulative mortality and Relative Percent Survival (RPS) in olive flounder Paralichthys olivaceus naturally infected with lymphocystis disease virus (LDV) after 8 weeks. Infected fish were intraperitoneally administered with 0, 5, 50, and 100 mg kg(-1) body weight of solvent extracts. In groups treated with 50 and 100 mg kg(-1) body weight, the chosen innate immune parameters significantly increased after 8 weeks when compared to 0 mg kg(-1) dose, but not with 5 mg kg(-1). Administration of P. granatum solvent extracts for 8 weeks significantly reduced the percentage mortality with the consequent increase in RPS. The results suggest that intraperitoneal administration of the leaf extracts of P. granatum at 50 or 100 mg kg(-1) dose clearly enhance the innate immune responses and disease resistance after 8 weeks in P. olivaceus against natural LDV infection.

Topics: Animals; Complement System Proteins; DNA Virus Infections; Fish Diseases; Flounder; Immunity, Innate; Iridovirus; Lythraceae; Muramidase; Phagocytosis; Plant Extracts; Respiratory Burst

We report the effect of two probiotics, Lactobacil and Sporolac as separate or mixed diets on innate immune mechanisms, such as phagocytosis activity, superoxide anion production of blood leukocytes, complement activity and plasma lysozyme activity, and disease resistance in lymphocystis disease virus (LCDV) infected olive flounder, Paralichthys olivaceus (523.5 +/- 18.3 g) on week 1, 2, and 4. In infected fish, administration of diet supplemented with Lactobacil individually or mixed with Sporolac significantly enhanced the immune parameters like phagocytic activity superoxide anion production, complement activity, and plasma lysozyme. However administration of supplemented diet with Sporolac alone, all the chosen immune parameters did not enhance when compared to control group; this diets resulted in lower mortality (30% and 25%) than Sporolac diet group (45%) in 30 days. We conclude that Lactobacil individually or mixed with Sporolac act as immunostimulants that enhance the innate immune response and disease resistance in lymphocystis disease virus (LCDV) infected olive flounder.

Topics: Analysis of Variance; Animals; Aquaculture; Dietary Supplements; DNA Virus Infections; Fish Diseases; Flounder; Immunity, Innate; Iridoviridae; Muramidase; Phagocytosis; Probiotics; Superoxides

In olive flounder, Paralichthys olivaceus infected with lymphocystis disease virus (LDV) bath treatment with formalin, hydrogen peroxide or Jenoclean at 50, 100, and 200 ppm daily 10 min for 60 days enhanced the innate immune response and disease resistance. Jenoclean enhanced the immune parameters at the lowest concentration of 50 ppm; on the other hand, hydrogen peroxide bath treatment enhanced the immunity level at 100 ppm, while formalin was effective only at 200 ppm. A low cumulative mortality and high relative percent survival was noted in Jenoclean treated group followed by formalin and hydrogen peroxide treated groups. In olive flounder at low concentrations of 50 ppm or 100 ppm hydrogen peroxide and Jenoclean effectively prevent LDV infection.

Topics: Analysis of Variance; Animals; Complement Pathway, Alternative; DNA Virus Infections; Dose-Response Relationship, Drug; Fish Diseases; Flounder; Formaldehyde; Hydrogen Peroxide; Immunity, Innate; Iridoviridae; Muramidase; Phagocytosis; Polymerase Chain Reaction; Republic of Korea; Respiratory Burst; Survival Analysis