muramidase and Corneal-Opacity

To study the immunohistochemical and clinical characteristics of the corneal epithelial cells implanted under the flap accompanied by the flap melting after excimer laser in situ keratomileusis (LASIK) and the method of management.. In eight eyes (In 4 eyes a part of the flap melted.) with corneal epithelial cells implanted under the flap after LASIK, the corneal flap was torn again. The implanted tissue was removed. After washing between the interfaces, the flap was repositioned. The implanted tissue was immunohistochemically stained by using four kinds of monoclonal antibodies and using terminal deoxyribonucleotide transferase-mediated dUTP nick end labeling (TUNEL) method.. Early in the course, the disorder appeared on the temporal side, expanded and thickened. The defective periphery of the corneal flap presented as a partial lunar eclipse. The melting process was self-limited in 3 months. The result was good after the implanted tissue was removed. The implanted material was stained by using HE, immunohistochemical and TUNEL methods. Some vesicle-like degeneration in the implanted layer was found. There was lysozyme in the vesicles, and the cells of implanted layer possessed a trend of apoptosis. All the cells were dead in the vesicles.. The progress of corneal epithelial implantation under corneal flap after LASIK may include 3 periods: epithelial implantation, corneal flap melting and self-limitation. Early in the course, the implanted material presented as a thin film. Afterwards, it becomes a non-structural material. Possibly the melting of the corneal flap is caused by apoptosis. Therefore, the implanted epithelium should be removed early.

Topics: Adult; Apoptosis; Corneal Opacity; Cyclin D1; Epithelium, Corneal; Female; Humans; Immunohistochemistry; In Situ Nick-End Labeling; Keratomileusis, Laser In Situ; Male; Muramidase; Proto-Oncogene Proteins c-bcl-2; Surgical Flaps; Visual Acuity

Topics: Child; Child, Preschool; Corneal Opacity; Diet Therapy; Edema; Hospitalization; Humans; Kwashiorkor; Leukocytes; Muramidase; Night Blindness; Proteins; Serum Albumin; Spectrophotometry; Syndrome; Vitamin A; Vitamin A Deficiency; Xerophthalmia

Topics: Animals; Anterior Chamber; Aqueous Humor; Biochemical Phenomena; Biochemistry; Carbohydrate Metabolism; Contusions; Cornea; Corneal Opacity; Muramidase; Polysaccharides; Research; Swine