muramidase and Bacteriuria

Topics: Anti-Bacterial Agents; Bacteriuria; Blood; C-Reactive Protein; Cerebrospinal Fluid; Complement System Proteins; Delivery, Obstetric; Female; Fetal Diseases; Humans; Immunoglobulins; Infant Care; Infant, Newborn; Infant, Newborn, Diseases; Infection Control; Infections; Inflammation; Leukocyte Count; Maternal-Fetal Exchange; Muramidase; Phagocytosis; Pregnancy; Sex Factors; Sterilization

In the course of 4 years we isolated 193 E. coli strains of 55 patients with chronic pyelonephritis. In patients with obstructive chronic pyelonephritis the mean value of the immunofluorescence titre (in the serum) to the E. coli strain excreted in the urine as well as the total complement were significantly increased, the serum lysozyme was significantly lower than in patients with non-obstructive chronic pyelonephritis. A relation to the activity of the disease was existing only in the non-obstructive chronic pyelonephritis, where in the active stage the total complement was significantly decreased, the complement factors C3 and C4 as well as the urine lysozyme were significantly increased in comparison to the inactive stage. 94.64% of all immunofluorescence titres obtained to the homologous strain in the patients' serum were above the border of the normal area of 1:40. A relation between level of the titre and activity of the disease could not be established. No significant differences could be proved between the titres taken to serum-sensitive and serum-resistant strains. In 32.73% of the patients we observed disturbances of the serum bactericidia against the homologous serum-sensitive E. coli urine strain at one or several points. They fall to equal shares to patients with obstructive and non-obstructive chronic pyelonephritis and were found at 66.67% in the active stage of the two forms of the disease. In patients with and without disturbances of bactericidia no significant differences in the total complement, in the complement factors C3 and C4, the C3-activator, the serum lysozyme and the immunofluorescence titres could be proved.

Topics: Antibody Formation; Bacteriuria; Blood Bactericidal Activity; Chronic Disease; Complement System Proteins; Escherichia coli; Escherichia coli Infections; Fluorescent Antibody Technique; Humans; Immunocompetence; Muramidase; Pyelonephritis

Urine from children with ileal conduit urinary diversions was assayed for antibody-coated bacteria, lysozyme and lactic dehydrogenase isoenzymes, and correlated with the results of quantitative urine culture. A significant proportion of the antibody-coated bacteria and lysozyme assays were positive when more than 10(4) organisms per ml. of urine were found. Lactic dehydrogenase isoenzyme results were frequently at variance with the other assay and culture results. Because none of the ancillary assays was invariably positive in patients with symptoms suggestive of upper tract disease, they remain imperfect research tools for localization of urinary tract infections in patients with ileal conduits. In this patient population quantitative urine culture remains the best clinical method for diagnosing urinary tract infections and evaluating the adequacy of antibiotic therapy. However, since the antibody-coated bacteria and lysozyme assays converted to negative with successful treatment, these tests may provide additional guidance to the physician in assessing the adequacy of treatment of urinary tract infections in patients with ileal conduit diversions.

Topics: Adolescent; Adult; Antibody-Coated Bacteria Test, Urinary; Bacteriuria; Child; Child, Preschool; Female; Humans; Ileum; Infant; Isoenzymes; L-Lactate Dehydrogenase; Male; Meningomyelocele; Muramidase; Urinary Diversion

Topics: Animals; Bacteriuria; Clinical Enzyme Tests; Escherichia coli Infections; Humans; Leukocytes; Muramidase; Pyelonephritis; Rabbits; Time Factors

Topics: Acute Disease; Bacteriuria; Child; Child, Preschool; Enterobacteriaceae Infections; Humans; Muramidase; Pseudomonas Infections; Staphylococcal Infections

Topics: Bacteriuria; Female; Humans; Male; Muramidase; Pyuria; Urinary Tract Infections

Topics: Adult; Bacteriuria; Female; Humans; Male; Muramidase; Urine

Topics: Adult; Aged; alpha-Glucosidases; Bacteriuria; Child; Female; Glucosidases; Humans; Malate Dehydrogenase; Male; Middle Aged; Muramidase; Pyelonephritis; Time Factors

A study was made of 111 strains of plasma-negative spathylococci isolated from the blood, pleural fluid, urine, and exudate of the abdominal cavity of 30 patients. The studies were carried out by 18 criteria. A variety of biological properties and signs characteristic of pathogenic staphylococci (hemolytic activity, anaerobic splitting of mannite, the presence of phosphatase, lysozyme, protease, alpha-toxin, fibrinolysin) were noted. A high resistance to tetracycline and penicillin was found in the strains isolated from the blood and the pleural cavity.

Topics: Animals; Ascitic Fluid; Bacteriophage Typing; Bacteriuria; Cross Infection; Erythrocytes; Fibrinolysin; Hemolysis; Humans; Mannitol; Muramidase; Penicillin Resistance; Penicillins; Phospholipases; Phosphoric Monoester Hydrolases; Pleural Effusion; Pyelonephritis; Rabbits; Sepsis; Staphylococcal Infections; Staphylococcus; Surgical Procedures, Operative; Tetracycline; Toxins, Biological

Topics: Bacteriuria; Glomerular Filtration Rate; Glomerulonephritis; Humans; Hypertension; Kidney Diseases; Muramidase; Pyelonephritis; Uremia; Urinary Calculi

Topics: Animals; Bacteriuria; Cystitis; Dog Diseases; Dogs; Edetic Acid; Gentamicins; Injections, Subcutaneous; Male; Muramidase; Pseudomonas aeruginosa; Pseudomonas Infections; Therapeutic Irrigation; Tromethamine; Urinary Catheterization; Urinary Tract Infections

Topics: Bacteriuria; Follow-Up Studies; Humans; Kidney Calculi; Muramidase; Paraplegia; Proteinuria; Pyelonephritis; Pyuria; Spinal Cord Injuries