muramidase and Ascites

Some urinary enzymes (NAG, AAP, lysozyme) considered to be sufficiently sensitive and reliable markers of renal damage were controlled in 20 patients with cirrhosis of the liver and in 20 healthy control subjects. The results, stated as mean +/- SD, showed a statistically very significant increase (p < 0.01) of NAG and lysozyme in cirrhotics. Furthermore, this increase could be at least in part related with the seriousness of clinical condition. On the basis of these results, we think the urinary dosage of NAG and lysozyme is, in the subjects with liver cirrhosis, a bloodless method to show an early renal damage.

Topics: Acetylglucosaminidase; Adult; Aged; Aged, 80 and over; Aminopeptidases; Ascites; Butyrylcholinesterase; CD13 Antigens; Clinical Enzyme Tests; Diabetes Mellitus; Female; Humans; Kidney Diseases; Liver Cirrhosis; Male; Middle Aged; Muramidase; Time Factors

The authors investigated specific and non-specific immunological parameters in malignant, cirrhotic, cardial and nephrogenic ascites. At the same time bacteriological and cytological examinations of ascites were made. In the submitted paper the authors compare, using statistical methods, immunological parameters of ascites in different patient groups and pay attention to their relationship with positive bacteriological and cytological findings in ascites. The authors recorded the largest number of positive bacteriological cultivation in cirrhotic and malignant ascites which correlates with lower values of some non-specific immunological parameters in ascites.

Topics: Antigen-Antibody Complex; Ascites; C-Reactive Protein; Escherichia coli; Humans; Immunoglobulins; Muramidase

Methods are described for the automated evaluation of affinity columns by frontal boundary analysis. These methods were used to evaluate the performance of immunoaffinity columns based on antilysozyme monoclonal antibody-lysozyme immunoaffinity system. This model system enabled the effects of (i) matrix activation and (ii) the density of immobilized antibody on the change in specific activity of immobilized antibody to be quantitatively assessed. Experimental data were accumulated with carbonyldiimidazole-activated Fractogel HW65F and Trisacryl GF2000 resins and cyanogen bromide-activated Sepharose 4B. An increase in the molar ratio between the concentration of the active groups on the activated matrix and the concentration of immobilized antibody ligands did not result in significant change in the specific activity of the immobilized antibody in the immunochromatographic system. However, increased antibody density with the Fractogel HW65F resin resulted in an increase in the apparent heterogeneity of antibody binding sites for lysozyme and a significant decrease in the specific activity of the immobilized antibody. Furthermore, data from size-exclusion studies with these immunoaffinity matrices demonstrated that at high antibody densities, the accessibility of the immobilized antibody was further decreased due to steric resistance as the antigen size increased.

Topics: Animals; Antibodies, Monoclonal; Ascites; Buffers; Chromatography, Affinity; Chromatography, Gel; Chromatography, High Pressure Liquid; Kinetics; Ligands; Mice; Mice, Inbred BALB C; Muramidase; Proteins

Topics: Animals; Ascites; Glycolipids; Humans; Lipids; Muramidase; Neoplasms; Phospholipids; Sarcoma; Sarcoma, Yoshida