muramidase and Abscess

Topics: Abdominal Abscess; Abscess; Humans; Muramidase

The failure of new, innovative, and often expensive tests to show the presence of intraabdominal infection caused us to reexamine the value of an inexpensive and almost forgotten one. Lysozyme is a bacteriolytic enzyme located within the lysosomes of phagocytic cells including leukocytes.. We measured serum concentrations of lysozyme by a standard turbidimetric method in both a murine model of intraabdominal infection and in trauma patients with intraabdominal abscesses or other acquired infections.. In mice with intraabdominal abscess secondary to cecal ligation and puncture (n = 35) serum lysozyme activity increased compared with sham-operated controls (n = 20; p < 0.001). In trauma patients with intraabdominal abscess after injury and surgery (n = 19), there was also an increase in serum lysozyme activity compared with controls (n = 15; p < 0.001) or with patients with lung infection (n = 21; p < 0.001). The increase of serum lysozyme activity occurred before intraabdominal sepsis was clinically apparent.. In this study, serum lysozyme concentration has a high specificity related to the presence of an intraabdominal abscess and is an indirect measure of the sequestration of leukocytes to the site of an established or developing collection of intraabdominal pus. The estimation of serum lysozyme may be an aid to differentiate critically ill patients with a potential occult intraabdominal abscess.

Topics: Abdomen; Abscess; Adult; Animals; Female; Humans; Male; Mice; Middle Aged; Muramidase

This study determines whether there are any differences in several immunologic parameters in circulating peripheral blood leukocytes, serum, and plasma compared with pus leukocytes, and supernatant of various types of abscess.. Although there have been reports of high lysozyme levels and low complement levels within pus, there has been no systemic comparison of concentrations of these substances and others within pus compared with those within peripheral blood.. Peripheral blood and abscess pus were collected from 31 patients with abscesses and percent and mean channel of monocytes expressing complement receptor and major histocompatibility antigen HLA-DR, the percent and mean channel of polymorphonuclear leukocytes (PMN) expressing complement receptor, lysozyme level, and levels of total hemolytic complement, iC3b, C5b-9, and immunoglobulins were measured within both pus and peripheral blood.. Percent of monocytes expressing HLA-DR and percent of monocytes and PMN expressing complement receptor, total hemolytic complement, and IgM were reduced within pus compared with peripheral blood, whereas the mean channel of monocytes expressing HLA-DR and the mean channel of PMNs expressing CR3, C5b-9, and lysozyme were increased in pus.. There are marked differences in immunologic parameters measured within pus of abscesses versus that seen in peripheral blood.

Topics: Abdomen; Abscess; Complement C3b; Complement Hemolytic Activity Assay; Complement Membrane Attack Complex; Drainage; Empyema, Pleural; HLA-DR Antigens; Humans; Immunoglobulin M; Leukocytes; Macrophage-1 Antigen; Muramidase; Prospective Studies; Superoxides; Suppuration

The use for local treatment in 45 patients with abscesses and phlegmons of lysozyme immobilized in polymethylsiloxane contributed to acceleration in wound cleaning of necrotic masses, active granulation and epithelization, reduction in duration of patients' treatment by 3-5 days.

Topics: Abscess; Adolescent; Adult; Anti-Infective Agents, Local; Cellulitis; Enzymes, Immobilized; Facial Dermatoses; Humans; Middle Aged; Muramidase; Neck; Occlusive Dressings; Wound Healing

Topics: Abscess; Adolescent; Adult; Antitoxins; Blood Bactericidal Activity; Cellulitis; Complement System Proteins; Humans; Middle Aged; Muramidase; Osteomyelitis; Otitis; Phagocytosis; Sinusitis; Staphylococcal Infections; Surgical Wound Infection; Tonsillitis; Ulcer

Topics: Abscess; Amino Acids; Animals; Cytoplasmic Granules; Electrophoresis; Guinea Pigs; Histocytochemistry; Immunity; Leukemia; Leukemia, Myeloid; Leukocytes; Lysosomes; Muramidase; Peritonitis; Rabbits; Ribonucleases