moxidectin and Trichostrongylosis

We studied the reproductive effects of administration of a long-acting antiparasitic (moxidectin) given to pre-pubertal ewe-lambs in Greece at the beginning of the reproductive season. 45 animals, naturally infected with trichostrongylids, were allocated into treated (n=30, treatment on D0, 21 June) or control (n=15) group. Rams of confirmed fertility, were introduced from 15 August (D55) to 20 December (D182) into the ewe-lambs. Throughout the study (performed at latitude N 36°26', in a flock free from brucellosis, Chlamydophila infection and toxoplasmosis), epg counts were monitored and reproductive performance of ewes was assessed. Up to D112, arithmetic mean epg counts in treated animals were 0; thereafter and up to D350, they were 23-473. Respective figures for controls were 190-977 epg. Reproductive performance parameters for treated and control animals respectively, were as follows; median 'Interval to first mating after ram introduction': 36.5 d and 71.0 (P=0.04); median 'Age at first mating': 8.5m and 10.0m (P=0.045); 'Cycling rate': 20.0% and 6.7% (P=0.03); 'Mating rate': 86.7% and 66.7%; 'Return-to-oestrus rate': 26.7% and 26.7%; 'Abortion rate': 3.3% and 0%; 'Lambing rate': 83.3% and 66.7%; 'Total lambs born per ewe' and 'Liveborn lambs born per ewe': 1.5 and 1.1 (P=0.01); 'Stillbirth rate' 0% and 0% and 'Lamb bodyweight per ewe': 5.0 kg and 3.8 kg (P=0.005). Anthelmintic treatment of pre-pubertal ewes, in order to maximise reproductive performance may be employed as a management strategy according to targets set in individual flocks.

Topics: Animals; Anthelmintics; Delayed-Action Preparations; Female; Macrolides; Pregnancy; Reproduction; Sheep; Sheep Diseases; Time Factors; Trichostrongylosis

Topics: Administration, Oral; Animals; Anti-Bacterial Agents; Antinematodal Agents; Goat Diseases; Goats; Haemonchiasis; Haemonchus; Macrolides; Nematode Infections; Ostertagia; Ostertagiasis; Trichostrongylosis; Trichostrongylus

Topics: Animals; Anthelmintics; Anti-Bacterial Agents; Antinematodal Agents; Equidae; Feces; Female; Horse Diseases; Horses; Intestinal Diseases, Parasitic; Ivermectin; Macrolides; Male; Parasite Egg Count; Pyrantel Pamoate; Strongylida Infections; Trichostrongylosis

Topics: Animals; Anthelmintics; Anti-Bacterial Agents; Dose-Response Relationship, Drug; Feces; Horse Diseases; Horses; Larva; Macrolides; Nematode Infections; Parasite Egg Count; Pilot Projects; Trichostrongylosis

The effect of maize grain supplementation on the resilience and resistance of browsing Criollo goat kids against gastrointestinal nematodes was evaluated. Five-month-old kids (n = 42), raised worm-free, were allocated to five groups: infected + not supplemented (I-NS; n = 10), infected + maize supplement at 108 g/d (I-S108; n = 8), maize supplement at 1% of body weight (BW) (I-S1%; n = 8), maize supplement at 1.5% BW (I-S1.5%; n = 8), or infected + supplemented (maize supplement 1.5% BW) + moxidectin (0.2 mg/kg BW subcutaneously every 28 d) (T-S1.5%; n = 8). Kids browsed daily (7 h) in a tropical forest for 112 days during the rainy season. Kids were weighed weekly to adjust supplementary feeding. Hematocrit (Ht), hemoglobin (Hb), and eggs per gram of feces were determined fortnightly. On day 112, five goat kids were slaughtered per group to determine worm burdens. Kids of the I-S1.5% group showed similar body-weight change, Ht and Hb, compared to kids without gastrointestinal nematodes (T-S1.5%), as well as lower eggs per gram of feces and Trichostrongylus colubriformis worm burden compared to the I-NS group (P > 0.05). Thus, among the supplement levels tested, increasing maize supplementation at 1.5% BW of kids was the best strategy to improve their resilience and resistance against natural gastrointestinal nematode infections under the conditions of forage from the tropical forest.

Topics: Anemia; Animal Feed; Animals; Anthelmintics; Disease Resistance; Female; Food, Fortified; Forests; Gastrointestinal Diseases; Goat Diseases; Goats; Helminthiasis, Animal; Intestinal Diseases, Parasitic; Larva; Macrolides; Male; Mexico; Nematode Infections; Parasite Egg Count; Random Allocation; Seasons; Trichostrongylosis; Zea mays

Faecal egg count reduction tests (FECRTs) were performed on 21 goat farms in Guadeloupe (FWI). Anthelmintic resistance (AR) to netobimin (benzimidazole) was found in all 15 herds in which it was tested. AR to ivermectin (avermectin) and levamisole (imidazothiazole) were also very largely spread (14 out of 17 farms and 7 out of 9 farms, respectively). AR to the final moxidectin (milbemycin) released was already present in 2 out of 9 farms in which it was tested. Haemonchus was the dominant genus of gastrointestinal nematodes and was more frequently found to be resistant to netobimin, ivermectin and moxidectin than Trichostrongylus, the latter appeared to be more often resistant to levamisole. A first survey 15 years ago revealed only AR to benzimidazoles and one suspected case of AR to ivermectin.

Topics: Animals; Anthelmintics; Benzimidazoles; Drug Resistance; Feces; Goat Diseases; Goats; Guadeloupe; Haemonchiasis; Haemonchus; Ivermectin; Levamisole; Macrolides; Parasite Egg Count; Surveys and Questionnaires; Trichostrongylosis; Trichostrongylus

We describe a case of anthelmintic resistance on one of the largest organic small ruminant farms in Denmark. The flock was established in 2007 by purchase of animals from other Danish farms and had history of clinical parasitism, high mortality of young stock and anthelmintic treatment failure. In October 2011, 40 lambs and 40 kids were selected for a faecal egg count reduction test (FECRT) with fenbendazole (FBZ), ivermectin (IVM), moxidectin (MOX) and levamisole (LEV). Lambs were treated with the recommended sheep dose of each product while kids received the sheep dose of IVM, 1.5× sheep dose of MOX and 2× sheep dose of FBZ and LEV. Untreated lambs and kids were also included and three methods for calculating faecal egg count (FEC) reduction were compared. In a subsequent investigation, a controlled efficacy test (CET) with FBZ and IVM was performed in lambs infected with Haemonchus contortus and Trichostrongylus colubriformis isolated from adult goats on the farm. Recovered specimens of H. contortus were subjected to pyrosequencing for detection of single nucleotide polymorphisms (SNPs) related to benzimidazole (BZ) resistance. During the FECRT, FECs in untreated lambs dropped significantly by 47%. No FEC reduction was detected in untreated kids. After FBZ treatments, FEC reductions in lambs and kids ranged from 15 to 54% and 49-56%, respectively, according to the different calculation methods. Post IVM treatments, FEC reductions in lambs and kids varied between 71-90% and 81-83%, correspondingly. LEV and MOX reduced FECs by 98-100% in both species. In the CET, FBZ reduced H. contortus worm counts by 52-56% and no reduction in T. colubriformis counts were detected after treatment. IVM eliminated 100% of H. contortus and reduced T. colubriformis counts by 84-92%, according to different calculation methods. Pyrosequencing of isolated H. contortus revealed increased frequencies of the BZ resistance-related SNP in codon 200 of the β-tubulin isotype 1 gene. Frequency of BZ resistance-related SNPs in codons 167 and 198 were very low and did not exceed levels as obtained in the susceptible reference isolate. Anthelmintic resistance was confirmed in this recently established organic farm and low field efficacy of FBZ was verified by CET and pyrosequencing. BZ-resistant populations of H. contortus and T. colubriformis were isolated for the first time in Denmark. Problems with correct dosing of goats, the observed FEC reduction in untreated lambs and the relevan

Topics: Animals; Anthelmintics; Benzimidazoles; Denmark; Drug Resistance; Female; Fenbendazole; Goat Diseases; Goats; Haemonchiasis; Haemonchus; Ivermectin; Levamisole; Macrolides; Male; Parasite Egg Count; Sequence Analysis, DNA; Sheep; Sheep Diseases; Trichostrongylosis; Trichostrongylus

Drug resistance in sheep gastrointestinal trichostrongyles is a cosmopolitan major constraint to small ruminant production. Despite reports that anthelmintic drug resistance has become common, there are limited information on the presence of drug resistance in Italy. The purpose of this study was to determine the effect of four anthelmintics to control infection in sheep in central Italy. Fifty sheep with fecal egg counts (FEC) > or =150 eggs per gram were selected on each of three farms (n = 150 total sheep) which were randomly allocated to one of five groups. Groups were treated with febantel, levamisole, ivermectin, or moxidectin while the fifth group acted as the control group. A FEC reduction test (FECRT) was conducted on each animal and the mean FEC of each treatment group was compared to that of the control group within farm. Resistance was declared when percentage reduction (R) <95% and the lower 95% confidence interval was <90%. Levamisole (mean R = 89%) resistance was found on all farms and ivermectin (mean R = 93%) resistance was found on two of the three farms. Posttreatment larval cultures showed the presence of Teladorsagia (Ostertagia) circumcincta and Trichostrongylus spp. larvae. Febantel (mean R = 96%) and moxidectin (mean R = 100%) remained effective. This study suggests that drug resistance in sheep gastrointestinal trichostrongyles is present in central Italy and a potential problem which would justify a broader nationwide geographical investigation.

Topics: Animals; Antinematodal Agents; Drug Resistance, Multiple; Feces; Guanidines; Italy; Ivermectin; Levamisole; Macrolides; Ostertagia; Ostertagiasis; Parasite Egg Count; Sheep; Sheep Diseases; Treatment Outcome; Trichostrongylosis; Trichostrongylus

The development of moxidectin resistance (MOX-R) in sheep parasitic gastrointestinal nematodes already carrying multiple resistances to other anthelmintic groups has made control of these strains very difficult. The anthelmintic resistance patterns of MOX-R strains of Trichostrongylus colubriformis and Haemonchus contortus were characterized to provide an insight into the remaining role of anthelmintics in the control of such strains. Homozygous MOX-R individuals of both genera were unaffected by moxidectin. For MOX-R heterozygotes a dose rate of 200 microg/kg abamectin (ABA) given orally removed 25% of H. contortus while 200 microg/kg MOX given orally achieved a 72% reduction. Doubling the dose rate of ABA improved the mean efficacy to 37%. Consequently, in H. contortus, the degree of dominance differs markedly between the two anthelmintics. A dose rate of 8 mg/kg levamisole and 185 mg/kg napthalophos achieved >95% reduction in worm count of the MOX-R homozygous H. contortus but only 85 and 7%, respectively against the MOX-R homozygous T. colubriformis.

Topics: Abomasum; Administration, Oral; Animals; Anthelmintics; Drug Resistance; Feces; Female; Haemonchiasis; Haemonchus; Ivermectin; Levamisole; Macrolides; Male; Organophosphorus Compounds; Parasite Egg Count; Queensland; Sheep; Sheep Diseases; Trichostrongylosis; Trichostrongylus

This study was conducted to evaluate the effects of strategic early season treatments with Moxidectin on trichostrongyles in first-season grazing heifer calves on a permanent pasture. Three groups of Black-Pied Friesian heifer calves were turned out in early May on a permanent pasture naturally infected with trichostrongyle larvae. Two of these groups were treated with Moxidectin at turnout or at turnout and again 8 weeks later, while one group served as untreated controls. For the first three weeks of the experiment all animals grazed together in one flock on one pasture. Subsequently, the pasture was divided into three comparable plots, which from then and until housing in mid October were grazed by each calf group. The results showed that the treatments significantly suppressed faecal egg excretion in the early part of the season, leading to reduced herbage infectivity and parasitism over the rest of the season. Results from faecal egg counts determined at genus level revealed that the persistent activity of Moxidectin appeared to be longer against Ostertagia spp. than against Cooperia spp. Two Moxidectin treatments, given at turnout and on week 8, protected the calves to a higher degree than a single Moxidectin treatment given at turnout. However, there was also a significant effect of only one Moxidectin treatment which possibly could be explained by a low overwintered larval population and a drought period in the early summer.

Topics: Animals; Anthelmintics; Anti-Bacterial Agents; Cattle; Cattle Diseases; Feces; Larva; Macrolides; Ovum; Pepsinogens; Seasons; Trichostrongyloidea; Trichostrongylosis

The efficacy of orally administered moxidectin was determined against four benzimidazole-resistant nematode isolates. At the start of the trial, 30 lambs were each infected experimentally with 20,000 third stage larvae (5000 Haemonchus contortus, 7000 Teladorsagia circumcincta, 3000 Trichostrongylus colubriformis and 5000 Cooperia curticei); 28 days later they were allocated randomly to three groups of 10: one untreated group, one group treated orally with fenbendazole (5 mg/kg bodyweight) and one group treated orally with moxidectin (0.2 mg/kg). Samples of faeces were taken five and 10 days after treatment and the lambs were killed 10 days after treatment. Fenbendazole reduced the average number of nematode eggs in faeces by 95 per cent and the average number of worms by 25 to 45 per cent according to the species. The efficacy of moxidectin against these benzimidazole-resistant isolates was 100 per cent. No adverse reactions to either of the drugs were observed.

Topics: Animals; Anthelmintics; Anti-Bacterial Agents; Benzimidazoles; Drug Resistance; Feces; Haemonchiasis; Intestinal Diseases, Parasitic; Macrolides; Male; Nematode Infections; Parasite Egg Count; Sheep; Sheep Diseases; Trichostrongyloidiasis; Trichostrongylosis

The anthelmintic efficacy of benzimidazoles, levamisole, closantel, ivermectin and moxidectin was evaluated on an institutional farm in Malaysia using faecal egg count reduction tests, controlled slaughter trials and an in vitro egg hatch assay. The results of this study indicated simultaneous resistance of Haemonchus contortus against benzimidazoles and ivermectin and of Trichostrongylus colubriformis against benzimidazoles and levaminsole on the same farm. Moxidectin was effective against the ivermectin resistant H. contortus.

Topics: Animal Husbandry; Animals; Anthelmintics; Anti-Bacterial Agents; Benzimidazoles; Drug Resistance; Haemonchiasis; Haemonchus; Ivermectin; Levamisole; Macrolides; Malaysia; Parasite Egg Count; Salicylanilides; Sheep; Sheep Diseases; Trichostrongylosis; Trichostrongylus

To determine whether there is mutual resistance to avermectin and milbemycin anthelmintics, ivermectin and moxidectin sheep drenches were tested against ivermectin-resistant and susceptible isolates of Ostertagia circumcincta and Trichostrongylus colubriformis in sheep. None of the isolates had been exposed to moxidectin previously. The dosage of ivermectin required to remove 95 per cent of the ivermectin-resistant O circumcincta and T colubriformis were 23 times and six times larger, respectively, than the dosages required to remove the same percentage of susceptible isolates. The dosages of moxidectin required to remove 95 per cent of the ivermectin-resistant O circumcincta and T colubriformis were 31 times and nine times larger, respectively, than the dosages required to remove the same percentage of susceptible isolates. It is concluded that the worms resistant to ivermectin were also resistant to moxidectin.

Topics: Administration, Oral; Animals; Anthelmintics; Anti-Bacterial Agents; Drug Resistance; Female; Ivermectin; Macrolides; Male; Ostertagia; Ostertagiasis; Random Allocation; Sheep; Sheep Diseases; Trichostrongylosis; Trichostrongylus

Three groups of 11 naturally infected crossbred beef calves were injected subcutaneously with moxidectin 1 per cent injectable at 0.2 or 0.3 mg moxidectin/kg bodyweight or with the unmedicated vehicle. Nematode infections had been acquired during grazing from December to April. Based on the faecal egg counts and total worm counts of the control calves at necropsy (11 to 13 days after treatment) most of the calves had heavy parasitic burdens. Ostertagia ostertagi was predominant and the mean numbers of adults, developing fourth stage larvae (L4) and inhibited early L4 were 45,906, 10,061 and 68,918, respectively. Haemonchus placei and Trichostrongylus axei were also present in the abomasa. Three species of Cooperia, Oesophagostomum radiatum L4 and T colubriformis adults were found in the intestinal tract. Both dosages of moxidectin were equally effective (P < 0.05) against all the abomasal nematodes (99.9 to 100 per cent) and the intestinal tract nematodes (99.4 to 100 per cent). No adverse reactions to the moxidectin treatment were observed. Abomasal pathology characteristic of heavy O ostertagi infection was observed in the control calves, but not in the treated calves.

Topics: Animals; Anthelmintics; Anti-Bacterial Agents; Cattle; Cattle Diseases; Feces; Female; Haemonchiasis; Intestinal Diseases, Parasitic; Macrolides; Nematode Infections; Oesophagostomiasis; Ostertagiasis; Parasite Egg Count; Trichostrongyloidiasis; Trichostrongylosis

A new anthelmintic assay is described which uses immunosuppressed (60 ppm hydrocortisone acetate in diet) rats infected with the nematode Trichostrongylus colubriformis. Immunosuppressed rats were infected with 1500 T. colubriformis larvae, treated either orally or subcutaneously on Day 14 post-infection and necropsied 4 days after treatment. The worm counts in immunosuppressed control animals averaged 775 worms per rat. A range of benzimidazoles, levamisole hydrochloride, morantel tartrate, 22,23-dihydroavermectin B1a and alpha-milbemycin have been evaluated in the assay. The ED95 values obtained indicate that rats infected with T. colubriformis provide a highly predictive model for assaying the activity of experimental drugs in vivo prior to studies in ruminants.

Topics: Animals; Anthelmintics; Anti-Bacterial Agents; Benzimidazoles; Disease Models, Animal; Feces; Immunosuppression Therapy; Ivermectin; Levamisole; Macrolides; Male; Morantel; Parasite Egg Count; Rats; Ruminants; Trichostrongylosis

A novel series of milbemycin antibiotics were produced by soil isolate, strain E225 which was shown to be a Streptomyces species. The antibiotics displayed anthelmintic activity against Trichostrongylus colubriformis in the gerbil. Two of the compounds, VM 44857 and VM 44866 were shown to be potent anthelmintics against mixed nematode infections in sheep.

Topics: Animals; Anthelmintics; Anti-Bacterial Agents; Chromatography, High Pressure Liquid; Fermentation; Gerbillinae; Haemonchus; Macrolides; Molecular Structure; Nematode Infections; Sheep; Sheep Diseases; Streptomyces; Trichostrongylosis