motilin and Stomach-Neoplasms

Topics: Bombesin; Cholecystokinin; Cimetidine; Epidermal Growth Factor; Gastric Inhibitory Polypeptide; Gastrins; Gastrointestinal Hormones; Humans; Motilin; Nerve Tissue; Pancreas; Pancreatic Polypeptide; Pituitary Gland; Secretin; Somatostatin; Stomach Neoplasms; Vasoactive Intestinal Peptide; Zollinger-Ellison Syndrome

To study the change of the serum gastrin (GAS) and plasma motilin (MTL) levels as well as the gastric motility in gastric cancer patients after subtotal gastrectomy.. We used radioimmunoassay method to detect the levels of serum GAS and plasma MTL in 39 healthy volunteers (control group) and 39 gastric cancer patients before and after gastrectomy (gastric cancer group). Electrogastrography (EGG) was measured at the same time points.. The levels of serum GAS and plasma MTL in gastric cancer group before operation were significantly higher than those in control group (P = 0.000), and the levels of serum GAS and plasma MTL in gastric cancer stage II patients were also significantly higher than those in stage III patients (P <0.05). The levels of serum GAS and plasma MTL in gastric cancer group significantly decreased on the first post-operative day (P = 0.000), but returned to the pre-operative levels on the seventh post-operative day. The incidence of the abnormity of gastric motility in gastric cancer group on the seventh post-operative day was significantly higher than the pre-operation incidence (89.7% vs. 43.6%; chi2 = 18.692, P <0.01).. The levels of serum GAS and plasma MTL transiently decreased in gastric cancer patients. Subtotal gastrectomy could affect the gastric motility in gastric cancer patients.

Topics: Adult; Aged; Female; Gastrectomy; Gastrins; Gastrointestinal Motility; Humans; Male; Middle Aged; Motilin; Radioimmunoassay; Stomach Neoplasms; Treatment Outcome

There has been no report concerning fasting Roux limb motilities and gut hormone in patients with early dumping syndrome (EDS) after total gastrectomy with Roux-en-Y reconstruction. The present study investigated interdigestive migrating motor complex, phase III (IMMC-pIII) in the Roux limb, changes in the plasma gut hormone levels, and the relationships among those parameters and EDS.. Forty-eight patients (35 men, 13 women; aged 33 to 69 years, mean, 59.8) 1.5 to 2.0 years (mean 1 year, 8 months) after total gastrectomy with Roux-en-Y reconstruction for early gastric cancer were allocated to two groups based on the presence or absence of EDS. The occurrence of IMMC-pIII was compared and changes in the plasma levels of both motilin (MT) and somatostatin (SOM) were monitored.. None of the patients with EDS (n = 8) were recognized as having IMMC-pIII. In the IMMC-pIII positive group, MT and SOM increased sequentially from phase I through IMMC-pIII. In the IMMC-pIII negative group, MT and SOM were significantly lower than in the IMMC-pIII positive group during phases I, II, and IMMC-pIII (P <0.05, P <0.01, P <0.01, respectively).. There is an absence of IMMC-pIII in patients with EDS, and both MT and SOM might be necessary for IMMC-pIII generation.

Topics: Adult; Aged; Anastomosis, Roux-en-Y; Dumping Syndrome; Female; Gastrectomy; Gastrointestinal Hormones; Gastrointestinal Motility; Humans; Jejunum; Male; Middle Aged; Motilin; Myoelectric Complex, Migrating; Neoplasm Staging; Somatostatin; Stomach Neoplasms

To assess the pharmacophysiological significance of the enteric nervous system and the responses of the human lower esophageal sphincter (LES) to motilin and cisapride, the mechanical responses of esophageal tissues from six patients with esophageal cancer and seven patients with gastric cancer were investigated. Circular muscle reactions were recorded to evaluate the in vitro esophageal responses to electrical field stimulation (EFS), motilin, and cisapride, evoking the adrenergic and cholinergic nerves before and after treatment with various autonomic nerve blockers. The findings of this study revealed that: cholinergic nerves are mainly involved in the regulation of enteric nerves in the steady state, while non-adrenergic non-cholinergic (NANC) inhibitory nerves also exist; motilin may act both via nerves and also directly on the LES smooth muscle; and cisapride releases acetylcholine from the end of the postganglionic fiber of the cholinergic nerve in human LES thereby inducing contraction of the LES. These results suggest that cholinergic and NANC inhibitory nerves play an important role in human LES, and that motilin and cisapride is clinically useful for improving the impaired LES of patients with gastroesophageal reflux.

Topics: Aged; Cholinergic Fibers; Cisapride; Esophageal Neoplasms; Esophagogastric Junction; Female; Gastrointestinal Agents; Humans; In Vitro Techniques; Intestines; Male; Middle Aged; Motilin; Piperidines; Stomach Neoplasms

This investigation, conducted on 35 patients with advanced-stage gastric cancer, included 28 men and 7 women with a mean age of 50.1 years; also studied were 33 normal subjects as controls: 26 men and 7 women with a mean age of 45.8 years. Samples of blood and gastric juice were collected at fasting and in gastroscopy respectively. Substance P (SP), beta-endorphin (beta-EP), vasoactive intestinal peptide (VIP), motilin (MTL), gastrin (GT), and leu-enkephalin (LEK) of the sera and gastric juices were measured by radioimmunoassay kits. In the patients, SP and beta-EP of serum and gastric juice, and VIP, MTL and LEK of gastric juice, were higher than in the normal subjects (p < 0.01); gastrin of serum and gastric juice were decreased (p < 0.01). Serum and gastric juice SP, beta-EP levels correlated negatively with the gastrin (r = 0.462-0.519, p < 0.05). These data support the assumption that study of the peptides of serum and gastric juice can show a clinically significant change in gastric cancer patients.

Topics: Adult; Aged; Endorphins; Female; Gastric Juice; Humans; Male; Middle Aged; Motilin; Neuropeptides; Stomach Neoplasms; Substance P; Vasoactive Intestinal Peptide

In order to study the changes of gut hormones and gastric motility in gastric cancer patients, the concentrations of motilin (MOT) in plasma and duodenal juice were measured by RIA, and the intragastric pressure of the pylorus, the body and the fundus of the stomach of 33 gastric cancer patients were measured using a WYY-B Manometer (produced by Areomedicine Research Institute, Beijing, China). The results showed that MOT concentrations of both plasma and duodenal juice were significantly higher than that of healthy controls (P < 0.05-0.01), and that of duodenal juice was about 3 times higher than in healthy controls. The basic intragastric pressure and the wave pressure of the pylorus, body and fundus of the patients were significantly lower than that of control subjects (P < 0.01). Almost all the intragastric wave pressure of the patients were of inhibition type, especially that of at or near the cancer masses. Our results indicate that there were marked drop in the stomach tension and motility in gastric cancer patient, while the increased MOT concentrations of plasma and duodenal juice were due to the pathological compensation.

Topics: Duodenum; Female; Gastrointestinal Motility; Humans; Intestinal Secretions; Male; Motilin; Pressure; Stomach; Stomach Neoplasms

A total of 87 surgical cases of gastric carcinoma including 3 carcinoid tumors were investigated with the methods of silver reaction and immunoperoxidase stain for 8 different brain-gut hormones. Argyrophil (AP) cells were demonstrated in 38 cases (44%), argentaffin (AF) cells in 18 (21%) and endocrine cells in 13 (14%). The occurrence of endocrine cells had no relation with histological types. Glicentin cells were demonstrated in 10 cases, somatostatin in 7, motilin in 3, beta-endorphin in 2 and gastrin in one. Endocrine cells appeared generally in small numbers except one carcinoid tumor which had numerous somatostatin cells. No single cell positive for more than two kinds of hormones could be demonstrated. Two undifferentiated carcinomas looking like carcinoid tumors had argyrophil cells and endocrine cells of either somatostatin or beta-endorphin. These results suggest that carcinoid-like carcinoma or endocrine cell carcinoma may lie on the intermediate state between carcinoma and carcinoid tumor.

Topics: Adenocarcinoma; Adenocarcinoma, Mucinous; Adult; Carcinoid Tumor; Endorphins; Female; Gastrins; Gastrointestinal Hormones; Glucagon; Histocytochemistry; Humans; Male; Microscopy, Electron; Middle Aged; Motilin; Proglucagon; Protein Precursors; Somatostatin; Stomach Neoplasms; Vasoactive Intestinal Peptide; Vasopressins

Correlation of intestinal motility and serum motilin after abdominal surgery was investigated experimentally and clinically. The results were summarized as follows: The recovery of intestinal motility was faster in the dogs simply laparotomized than gastrectomized. The recovery of the small intestinal motility was followed by that of the large intestinal motility in the dogs. The intravenous infusion of the synthetic motilin markedly stimulated the intestinal motor activity in early postoperative period. Serum motilin levels significantly dropped one after abdominal surgery and rose again both in the dogs and the patients. The rate of increase of serum motilin levels at the postoperative period closely correlated with the recovery of the intestinal motility both in the dogs and the patients, that is, the higher the rate of increase was, the faster the recovery was.

Topics: Abdomen; Aged; Animals; Dogs; Gastrectomy; Gastrointestinal Hormones; Gastrointestinal Motility; Humans; Laparotomy; Male; Middle Aged; Motilin; Postoperative Period; Stomach Neoplasms

Plasma motilin levels were measured by radioimmunoassay both pre- and postoperatively in 37 patients who underwent abdominal surgery. In 8 colorectal cancer patients with tumor removal and in 13 stomach cancer patients with total gastrectomy by Roux-en-Y anastomosis or subtotal gastrectomy of Billroth I anastomosis, the motilin levels decreased before the onset of postoperative peristalsis, and at the time when peristaltic sounds could be detected stethoscopically, the plasma motilin levels had increased by 200% of the preoperative level. Similar motilin levels were maintained until the time of the initial postoperative flatus and/or stool. Thereafter, plasma motilin levels decreased and returned to almost the same levels as the preoperative ones. The postoperative time course of plasma motilin in 10 gallstone patients was very similar to that in the 21 gastrointestinal cancer patients above. In 5 stomach cancer patients with subtotal gastrectomy of Billroth II anastomosis, however, the peak at the time of the initial postoperative peristalsis was not as remarkable as that in the 13 stomach cancer patients.

Topics: Adult; Aged; Cholelithiasis; Colonic Neoplasms; Female; Gastrectomy; Gastrointestinal Diseases; Gastrointestinal Hormones; Humans; Male; Middle Aged; Motilin; Peristalsis; Radioimmunoassay; Rectal Neoplasms; Stomach Neoplasms