morphine and Cytomegalovirus-Infections

Topics: Chorioamnionitis; Chorionic Villi; Cytomegalovirus Infections; Female; Humans; Inflammation; Meconium; Obstetric Labor, Premature; Placenta; Placenta Diseases; Placental Circulation; Pregnancy; Pregnancy Complications, Infectious; Syphilis

Topics: Adult; Cytomegalovirus Infections; Digestive System Abnormalities; Female; Gestational Age; Humans; Infant, Premature, Diseases; Intestinal Volvulus; Meconium; Peritonitis; Respiratory System Abnormalities; Ultrasonography, Prenatal

Congenital cytomegalovirus (CMV) infection is a leading cause of hearing loss and mental retardation throughout the world. Detection of the CMV DNA by polymerase chain reaction (PCR) offers a sensitive, rapid, and specific means of identification. Meconium, the stool formed in utero, may be an ideal specimen for CMV detection. The objective of this study was to develop a PCR-based methodology for the detection of CMV in the meconium of neonates.. Meconium was collected from 10 newborn infants (seven with positive viral cultures and three uninfected infants born to CMV-seropositive mothers). For each, DNA was isolated from meconium by organic extraction and attachment to a DNA-binding matrix, and PCR was performed using amplimers specific for the major intermediate early (MIE) and late antigenic (LA) regions of CMV.. Gel electrophoresis demonstrated an anticipated PCR product of 250 base pairs (bp) corresponding to the MIE region of CMV in all infected and positive control meconium samples. Furthermore, a single band of 150 bp corresponding to the LA region of CMV was also amplified in several of the infected infants. Conversely, no amplification of these antigenic regions was noted in either uninfected infants born to CMV-seropositive mothers or negative controls.. CMV is present within the meconium of infected neonates and is readily detectable by PCR.

Topics: Cytomegalovirus; Cytomegalovirus Infections; DNA, Viral; Electrophoresis, Agar Gel; Female; Humans; Infant, Newborn; Meconium; Polymerase Chain Reaction; Pregnancy; Sensitivity and Specificity

Recent reports have suggested that focal hyperechoic abdominal masses detected during the second trimester may represent a normal variation in fetal intestinal development that is transient in nature and not associated with pathologic conditions. The patient described here had second-trimester ultrasonic findings of fetal meconium peritonitis without ascites, polyhydramnios, or other anomalies. Subsequent ultrasound examinations at 22, 30, and 36 weeks demonstrated no change in the abdominal appearance. At birth, this preterm male infant had clinical symptoms of congenital cytomegalovirus infection confirmed by viral culture and serologic studies. Retrospective studies of maternal serum obtained early in the second trimester confirmed a primary cytomegalovirus infection 4 weeks before the initial ultrasound examination. Although fetal hydrops and ascites have occasionally been associated with intrauterine cytomegalovirus infection, fetal meconium peritonitis has not been previously recognized in patients with congenital cytomegalovirus.

Topics: Adult; Cytomegalovirus Infections; Female; Fetal Diseases; Humans; Immunoglobulin Allotypes; Immunoglobulin G; Infant, Newborn; Male; Meconium; Peritonitis; Pregnancy; Pregnancy Trimester, Second; Ultrasonography, Prenatal