morphine and Cross-Infection

Nosocomial sepsis is the main problem that preterms have to face during their stay at neonatal intensive care units (NICU).. Twenty-six preterm infants born with a gestational age ≤ 32 weeks and/or weigh ≤1500 g were included in the study. Samples of meconium and feces (. A total of 179 . The local ethic committee approved this trial with the reference 09/157.

Topics: Anti-Bacterial Agents; Cluster Analysis; Cross Infection; DNA, Bacterial; Enteral Nutrition; Evolution, Molecular; Feces; Female; Genotyping Techniques; Humans; Infant; Infant, Newborn; Infant, Premature; Intensive Care Units, Neonatal; Male; Meconium; Microbial Sensitivity Tests; Phylogeny; Sepsis; Serratia Infections; Serratia marcescens

The aim of the study was to evaluate the potential colonization of nosocomial bacteria in enteral feeding systems and its effect on early gut colonization of preterm neonates.. Mother's own milk, donor milk, and preterm formula samples obtained after passing through the external part of the enteral feeding tubes were cultured. In addition, meconium and fecal samples from 26 preterm infants collected at different time points until discharge were cultured. Random amplification polymorphism DNA and pulse field gel electrophoresis were performed to confirm the presence of specific bacterial strains in milk and infant fecal samples.. Approximately 4000 bacterial isolates were identified at the species level. The dominant species in both feces from preterm infants and milk samples were Staphylococcus epidermidis, S aureus, Enterococcus faecalis, E faecium, Serratia marcescens, Klebsiella pneumoniae, and Escherichia coli. All of them were present at high concentrations independently of the feeding mode. Random amplification polymorphism DNA and pulse field gel electrophoresis techniques showed that several bacteria strains were found in both type of samples. Furthermore, scanning electron microscopy revealed the presence of a dense bacterial biofilm in several parts of the feeding tubes and the tube connectors.. There is a sharing of bacterial strains between the neonates' gastrointestinal microbiota and the feeding tubes used to feed them.

Topics: Cross Infection; Enteral Nutrition; Feces; Female; Humans; Infant; Infant, Newborn; Infant, Premature; Intestines; Male; Meconium

Topics: Cross Infection; Germany, East; Humans; Infant, Newborn; Infant, Premature, Diseases; Klebsiella Infections; Meconium