morphine and Colonic-Neoplasms

A rare case of a newborn infant with leiomyosarcoma of the transverse colon is reported. The condition was associated with meconium peritonitis due to a perforation proximal to a portion of the transverse colon that was completely surrounded by the tumor. The 12 previously published cases of leiomyosarcoma of the colorectum in childhood are reviewed, and the pathogenesis of meconium peritonitis is discussed.

Topics: Colonic Neoplasms; Female; Humans; Infant, Newborn; Leiomyosarcoma; Meconium; Peritonitis

Congenital infantile fibrosarcoma is a rare soft tissue malignancy that occurs in both axial and extremity locations. We report a case of this tumor arising from the left colon in a newborn presenting with an intrauterine perforation and meconium peritonitis.

Topics: Colonic Neoplasms; Diagnosis, Differential; Fibrosarcoma; Humans; Infant, Newborn; Laparotomy; Male; Meconium; Peritonitis

Antigenic reactivities of 4 purified CEA preparations and 4 different CEA-related normal antigens (NCA from lungs, NCA-2 from meconium, and NFA-1 and NFA-2 from adult feces) were comparatively analyzed with seven commercially available EIA kits [Abbott CEA-EIA Monoclonal, D-ZYME CEA, Imzyne CEA, CEA MITSUI II, CEA Roche EIA, Immunoball-CEA (N), and Glaozyme CEA]. All kits employ a sandwich-type solid-phase method using polystyrene beads and monoclonal anti-CEA antibodies as either capture antibody and/or tracer antibody. In general, all CEAs reacted well with these assay kits. Reactivity differences in weight among the CEA preparations were, however, observed in all but one assay kit (D-ZYME CEA) in which all CEAs showed fairly homogeneous reactivity. The degree of reaction intensity among the preparations used varied depending on the assay kits used. NCA, which is well known to be partially cross-reactive with CEA, revealed a negligible reactivity in all kits. NFA-1 which is also partially cross-reactive with CEA but antigenically unrelated to NCA, showed very strong reactivity in 2 kits [CEA Roche EIA and Immunoball-CEA (N)] and weak reactivity in another kit (CEA MITSUI II). NCA-2 in meconium and NFA-2 in normal adult feces, which both have immunochemical and physicochemical properties very similar to those of CEA, reacted to a greater or lesser extent with all kits. However, one assay kit (D-ZYME CEA), whose reactivity to different CEAs was fairly homogeneous, could discriminate CEAs from NCA-2 and NFA-2. Although the reactivity of NFA-2 was less than that of any CEA in all kits, that of NCA-2 was higher than that of some CEAs in 3 kits (Abbott CEA-EIA Monoclonal, CEA MITSUI II, and CEA Roche EIA). These differences in reactivity and specificity of currently available EIA kits for CEA should be borne in mind when selecting an assay kit.

Topics: Antibodies, Monoclonal; Antigens; Carcinoembryonic Antigen; Chromatography, High Pressure Liquid; Colonic Neoplasms; Cross Reactions; Feces; Humans; Immunoenzyme Techniques; Liver Neoplasms; Lung; Meconium; Molecular Weight; Reagent Kits, Diagnostic

The expression of 4 meconial antigens--beta-1-MA, beta-2-MA, MMA, gamma-MA in neoplastic and preneoplastic colonic mucosa was studied. Meconial antigens were revealed in small amount in adult normal colonic epithelium. The mucosa adjacent to tumours was rich in these antigens. Cellular localization of meconial antigens in these parts of colonic mucosa was similar to that of embryonal alimentary tract. beta-1-MA was found in 96%, beta-2-MA--73.6%, MMA--82.8%, gamma-MA--31.8% of colonic carcinomas.

Topics: Antigens; Antigens, Neoplasm; Colon; Colonic Neoplasms; Humans; Immunoenzyme Techniques; Intestinal Mucosa; Meconium; Precancerous Conditions

A rabbit antiserum to the meconium antigen of the human fetal intestine is obtained by immunization of rabbits by this substance preparation. The preparation is extracted from meconium by the sulphate ammonium (70% saturation) treatment and gel filtration of supernatant with selection of the 240 kD fractions. In the indirect immunoperoxidase reaction the antiserum reacts with epithelium of certain normal and fetal tissues. It displays oncofetal features in colon, since it reacts with fetal and malignant epithelium and does not react with normal epithelium of this organ. The developed test-system may be used in pathomorphology for the morphological and functional state of colon mucosa evaluation.

Topics: Absorption; Animals; Antibody Specificity; Antigens; Antigens, Neoplasm; Chromatography, Gel; Colon; Colonic Neoplasms; Electrophoresis, Polyacrylamide Gel; Epithelium; Humans; Immune Sera; Immunization; Immunoenzyme Techniques; Infant, Newborn; Meconium; Rabbits

This paper reports the presence of GM2 ganglioside containing N-glycolylneuraminic acid (NeuGc) in human colon cancer tissues. GM2(NeuGc) was detected by two-dimensional thin layer chromatography (2d-TLC)/enzyme-immunostaining using affinity-purified chicken antibody against GM3(NeuGc) and horseradish peroxidase-conjugated rabbit anti-chicken IgG antibody. Like usual GM2 ganglioside containing N-acetylneuraminic acid (NeuAc) isolated from Tay-Sachs brain, GM2(NeuGc) in colon cancer could be converted into GM3(NeuGc) by human kidney beta-N-acetylhexosaminidase A in the presence of a GM2-specific activator protein isolated from guinea pig kidney. Three of 7 specimens of Hanganutziu-Deicher (HD) antigen-positive human colon cancer tissues so far examined expressed this unique ganglioside. In order to detect and determine specifically GM2(NeuGc) on human colon cancers, specific antibody against GM2 (NeuGc) has been prepared by immunizing chickens. By a sensitive TLC/immunostaining method using the antibody, the amounts of the antigen were determined to be 0.3-3% of total lipid-bound sialic acid. NeuGc-containing gangliosides were also detected in meconium and fetal intestinal tissues. Three species of antigenic gangliosides in pooled meconium were tentatively identified as GM3(NeuGc), sialylparagloboside and sialylhexaosylceramide on the basis of their migration positions on 2d-TLC and the results of endo-beta-galactosidase treatment. GM3(NeuGc) was the sole HD-active ganglioside in fetal intestinal tissue from one of 3 individuals tested; the other two showed no HD-active ganglioside at all. GM2(NeuGc), however, could not be detected in either meconium or fetal tissues so far examined, suggesting that this unique ganglioside is a tumor-specific antigen, at least for human intestinal tissues.

Topics: Antibodies; Antigens, Heterophile; Antigens, Neoplasm; Colonic Neoplasms; G(M2) Ganglioside; Gangliosides; Humans; Intestines; Meconium; Neuraminic Acids

In a previous study, five monoclonal antibodies against the carcinoembryonic antigen (CEA) with different epitope specificities were delineated. One of these antibodies which exhibits a high affinity for CEA binds to different carcinoma tissues, to liver tissue, and to granulocytes. This antibody was selected for the immunoaffinity purification of CEA and related antigens from colorectal carcinoma tissue, from spleen tissues, from bile, and from meconium. After elution from the immunosorbent, the antigens were separated by SDS-PAGE, were transferred to nitrocellulose, and were incubated with the five different antibodies. Antibody T84.1 bound to the following antigens: 177 kD and 128 kD from colonic carcinoma, 81 kD from bile, 49 kD from spleen, as well as 165 kD and 100 kD from meconium. Two additional antibodies showed a similar binding pattern. The fourth antibody (CEA.11) bound to the 165 kD meconium antigen and to the two colorectal carcinoma antigens. The fifth antibody (T84.66) showed a strong reaction with the 177 kD colorectal carcinoma antigen and a faint reaction with a 183 kD antigen in meconium. As judged from m.w. and immunochemical properties, the 128 kD colorectal carcinoma antigen and the 100 kD meconium antigen are two novel CEA-related antigens. Because antibody CEA.11 did not bind to the 100 kD meconium antigen in Western blots, the 165 kD antigen could be eluted from a CEA.11 immunosorbent without contamination by the 100 kD antigen. Similarly, as predicted from the binding pattern in the Western blots, the two colorectal carcinoma antigens were separated from each other by a T84.66 immunosorbent.

Topics: Antibodies, Monoclonal; Binding Sites, Antibody; Carcinoembryonic Antigen; Carcinoma; Chromatography, Affinity; Collodion; Colonic Neoplasms; Electrophoresis, Polyacrylamide Gel; Humans; Meconium; Models, Biological; Molecular Weight; Paper; Rectal Neoplasms

Reactivity of neuraminidase-treated colorectal carcinoma cells with antibodies that detect the X-carbohydrate structure was greater than the reactivity of untreated cells. The same results were obtained with glycolipid extracts of meconium, a colorectal carcinoma cell line, three freshly excised human adenocarcinomas, and normal bronchial mucosa. The glycolipid was either not expressed or expressed in smaller quantities on the corresponding normal colon tissue. Further study showed that the major sialo-X glycolipid has six sugars including a single sialic acid which blocks X-antigenicity. These glycolipids were further analyzed by ion-exchange high-pressure liquid chromatography and thin-layer chromatography. These monosialo-X glycolipid antigens might serve as potential tumor markers.

Topics: Adenocarcinoma; Antibodies, Monoclonal; Antigens, Neoplasm; Antigens, Surface; Bronchi; Cell Line; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Colonic Neoplasms; Fetal Proteins; Glycolipids; Humans; Infant, Newborn; Meconium; Mucous Membrane; Neuraminidase; Radioimmunoassay

Two antigens cross-reactive with carcinoembryonic antigen (CEA) and distinct from the nonspecific cross-reacting antigen were identified in meconium by double immunodiffusion with a conventional goat anti-CEA antiserum. These two antigens together competitively inhibited cross-reacting antibodies against them in CEA radioimmunoassay and contributed to the measurement of meconium CEA levels which averaged 6 times higher than that determined with anti-CEA specific antibody. A purification method for one of these antigens, tentatively designated meconium antigen, is described and uses a combination of ethanol fractionation, ion-exchange and molecular sieve chromatography, and adsorption to an immunoadsorbent containing a cross-reactive murine monoclonal antibody to CEA. Preliminary characterization of the purified meconium antigen showed it to be a glycoprotein, migrating as an alpha-globulin and having a molecular size similar to that of CEA (Mr 185,000 versus 200,000). Antigenically, it lacks at least one determinant present on CEA and differs further from CEA by being weakly reactive with concanavalin A and resistant to proteolytic digestion with Pronase E. Although these properties of meconium antigen suggest that it may be nonspecific cross-reacting antigen 2, additional chemical and antigenic studies are required to establish its relationship to CEA and other CEA-related antigens in meconium.

Topics: Adenocarcinoma; Antigens; Carcinoembryonic Antigen; Colonic Neoplasms; Humans; Immunodiffusion; Infant, Newborn; Liver Neoplasms; Meconium; Radioimmunoassay

Topics: Carcinoembryonic Antigen; Colitis, Ulcerative; Colon; Colonic Neoplasms; Crohn Disease; Epitopes; Humans; Immunity, Cellular; Intestines; Isoelectric Focusing; Meconium; Neoplasms; Radioimmunoassay

Topics: ABO Blood-Group System; Animals; Antigens; Blood; Carcinoembryonic Antigen; Carcinoma; Chromatography, Gel; Colitis, Ulcerative; Colon; Colonic Neoplasms; Cross Reactions; Epitopes; Female; Genital Neoplasms, Female; Humans; Immune Sera; Immunodiffusion; Immunoelectrophoresis; Intestinal Mucosa; Liver; Meconium; Proteins; Pyuria; Rabbits; Saliva; Urine

Topics: Antigens; Carcinoembryonic Antigen; Colonic Neoplasms; Duodenal Neoplasms; Esophageal Neoplasms; Feces; Female; Glycoproteins; Humans; Immunodiffusion; Infant, Newborn; Meconium; Pancreatic Neoplasms; Pregnancy; Rectal Neoplasms; Uterine Neoplasms

Topics: ABO Blood-Group System; Animals; Antibody Specificity; Blood; Carcinoembryonic Antigen; Colon; Colonic Neoplasms; Complement Fixation Tests; Dextrans; Electrophoresis, Starch Gel; Female; Goats; Humans; Immune Sera; Immunodiffusion; Infant, Newborn; Meconium; Pregnancy; Rabbits; Tissue Extracts

Topics: Animals; Antigens; Carcinoembryonic Antigen; Colonic Neoplasms; Cross Reactions; Feces; Gastric Mucosa; Gastrointestinal Neoplasms; Humans; Immune Sera; Immunodiffusion; Immunoelectrophoresis; Lung; Meconium; Perchlorates; Radioimmunoassay; Sheep

Topics: Antigens, Neoplasm; Carcinoembryonic Antigen; Colon; Colonic Neoplasms; Humans; Hydrogen-Ion Concentration; Infant, Newborn; Isoelectric Focusing; Meconium; Methods; Neoplasm Proteins; Proteins; Radioimmunoassay; Sodium Chloride; Temperature; Time Factors; Tissue Extracts; Urea