monensin and Biliary-Fistula

Data regarding the action of monensin on the concentrations of glycerophospholipids and cholesterol in bile of rats subjected to total biliary diversion for 3 h are reported. After monensin their concentration in bile drops significantly in the first 60 min collections with respect to the control. Differences seem to be produced between the rates of transport to the bile of glycerophospholipids and cholesterol, not sufficiently explained by the inhibition of bile salt uptake determined by monensin at the sinusoidal pole of the hepatocyte.

Topics: Animals; Bile; Bile Ducts; Biliary Fistula; Biological Transport; Cholesterol; Female; Kinetics; Monensin; Phosphatidic Acids; Rats; Rats, Inbred Strains

To measure absorption of monensin or its metabolites and its elimination from the body, [14C]monensin sodium was given orally (1 mg/kg body wt) to two bile-fistulated ponies and iv (8.7 mg) to one bile-fistulated pony. For one orally-dosed pony, 4.7% of the 14C was eliminated in bile, 52% in feces, .7% in urine and 33% remained in the gastrointestinal (GI) tract after 3 d. Total 14C recovery was 90%. For the other orally-dosed pony, 18.3% of the 14C was eliminated in bile, 69% in feces, 1.7% in urine and 7% remained in the GI tract after 4 d. Total 14C recovery was 98%. For the iv-dosed pony, 72% of the 14C was eliminated in bile, .5% in feces, 4.9% in urine and 15% remained in the GI tract after 23 h. Total 14C recovery was 96%. Maximum rate of 14C elimination in feces from the orally-dosed ponies occurred on the third and fourth days, and the maximum rate of biliary elimination occurred between 16 and 24 h for one pony and 48 and 56 h for the other. For the iv-dosed pony, 14C became immeasurable in plasma within 12 h after dosing and appeared in bile within 30 min, with maximum biliary concentration occurring at about 1 h. Monensin accounted for a minimum of 19% of the 14C in a 56- to 72-h sample of feces for one orally-dosed pony and a minimum of 25% of the 14C in feces from the same time period for the other.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Administration, Oral; Animals; Bile; Biliary Fistula; Female; Furans; Horses; Injections, Intravenous; Intestinal Absorption; Monensin