mk-2206 and Long-QT-Syndrome

In long QT syndrome type 2, women are more prone than men to the lethal arrhythmia torsades de pointes. We previously reported that 17β-estradiol (E2) up-regulates L-type Ca. The purpose of this study was to investigate the molecular mechanisms whereby E2 up-regulates I. H9C2 and rat myocytes were incubated with E2 ± ER antagonist, or inhibitors of downstream transcription factors, for 24 hours, followed by western blots of Cav1.2α1C and voltage-clamp measurements of I

Topics: Animals; Blotting, Western; Calcium Channels, L-Type; Carrier Proteins; Cell Line; Chromones; CREB-Binding Protein; Disease Models, Animal; DNA; Enzyme Inhibitors; Estradiol; Estrogens; Gene Expression Regulation; Heterocyclic Compounds, 3-Ring; Long QT Syndrome; Morpholines; Myocytes, Cardiac; Patch-Clamp Techniques; Proto-Oncogene Proteins c-akt; Rats; Rats, Sprague-Dawley; Signal Transduction; Up-Regulation