minocycline and Dental-Caries

The purpose of this study was to introduce antibacterial property to pits and fissure sealant (PFS) in order to mitigate the major clinical problems associated with PFS, such as microleakage and secondary caries. We prepared a pH reliant cobalt oxide nanoparticle incorporated with minocycline (MNC@CO) and characterized to investigate its antibacterial potential against Streptococcus sobrinus. The physiochemical, morphological, and drug release kinetics at different pH (7.4, 5.0, and 3.5) from nanoparticles were investigated. The MNC@CO were added at 2.5% and 5.0% into experimental PFS and characterized for their antibiofilm capacity, biocompatibility, and mechanical properties including compressive and flexural strength. The groups 2.5% and 5.0% has shown statistically significant antimicrobial capacity against S. sobrinus compared to control (p < .05). The highest percentage of MNC release at different pH (especially at pH 5.0 and 3.5) was observed from 5.0% MNC@CO doped PFS. The PFS doped with 2.5% MNC@CO showed a highest compressive strength (110 MPa) over a period of 70 days as compared to 5.0% MNC@CO (75 MPa) and control (80 MPa). The flexural strength of both experimental groups was lower for both time points (24 h and 30 days) than control. In conclusion, the present study found that 2.5% MNC@CO doped PFS showed considerable anti-biofilm potential without compromising mechanical properties.

Topics: Anti-Bacterial Agents; Cobalt; Dental Caries; Humans; Minocycline; Nanoparticles; Oxides; Pit and Fissure Sealants

To evaluate the clinical and radiographic success rates of three mixed antibiotics in the non-instrumentation endodontic treatment of primary mandibular molars at 24-27 months postoperatively.. Eighty cariously involved lower primary molars from 58 children (ages 3-8 years) received a 3Mix medicament by non-instrumentation endodontic treatment and were then sealed with glass-ionomer cement and composite resin before permanent restoration with stainless steel crowns. The patients received a clinical and radiographic assessment every 6 months over a 2-year follow-up period with an intra-examiner reliability of 0.83-1.00 (κ value).. In 60 cases at 24- to 27-month follow-up, the success rates as determined by clinical and radiographic evaluation were 75% and 36.7%, respectively; however, the overall success rate of 3Mix non-instrumentation endodontic treatment was 36.7% with 15.8% of cases demonstrating a pulpal response of internal resorption.. Non-instrumentation endodontic treatment using 3Mix-MP showed good clinical success but had a low success rate based on radiographic evaluation at 2-year follow-up. Hence, 3Mix antibiotic treatment cannot replace a conventional root canal treatment agent as a long-term therapy.

Topics: Anti-Bacterial Agents; Anti-Infective Agents; Bisphenol A-Glycidyl Methacrylate; Child; Child, Preschool; Ciprofloxacin; Composite Resins; Crowns; Dental Alloys; Dental Caries; Dental Materials; Dental Pulp Exposure; Dental Pulp Necrosis; Drug Combinations; Follow-Up Studies; Glass Ionomer Cements; Humans; Mandible; Metronidazole; Minocycline; Molar; Organophosphates; Pulpotomy; Radiography, Bitewing; Resins, Synthetic; Root Canal Irrigants; Stainless Steel; Tooth, Deciduous; Treatment Outcome

The aim of this study was to clarify the antibacterial effect of a mixture of ciprofloxacin, metronidazole and minocycline, with and without the addition of rifampicin, on bacteria taken from infected dentine of root canal walls. The efficacy was also determined against bacteria of carious dentine and infected pulps which may the precursory bacteria of infected root dentine. This efficacy was estimated in vitro by measuring bacterial recovery on BHI-blood agar plates in the presence or absence of the drug combination. Bacteria ranging in number from 10(2) to 10(6) occurred in samples of infected root dentine (27 cases). However, none was recovered from the samples in the presence of the drug combination at concentrations of 25 micrograms ml-1 each. The respective drug alone (10, 25, 50 and 75 micrograms ml-1) substantially decreased the bacterial recovery, but could not kill all the bacteria. Bacteria taken from carious dentine (25 cases) and infected pulps (12 cases) were also sensitive to the drug combination. These results may indicate that the bactericidal efficacy of the drug combination is sufficiently potent to eradicate bacteria from the infected dentine of root canals.

Topics: Anti-Bacterial Agents; Anti-Infective Agents; Anti-Infective Agents, Local; Bacteria, Anaerobic; Ciprofloxacin; Dental Caries; Dental Pulp Cavity; Dentin; Drug Combinations; Humans; Metronidazole; Microbial Sensitivity Tests; Minocycline; Periapical Diseases; Pulpitis; Rifampin

Two antimicrobial agents, 9-aminoacridine (0.2%) and minocycline (0.2%), were evaluated for their efficacy in inhibiting root surface caries, bone loss, and microflora in rice rats. A solution of 5000 ppm fluoride was used as a positive control for the inhibition of root surface caries, and double-distilled water was used as a negative control group. Each rat was treated by having its molar teeth swabbed 2 X per day with the prescribed agent in its group for nine weeks. Root caries reduction in the minocycline and fluoride groups was not significantly different, but the reduction was significantly greater than in the 9-aminoacridine group, with the caries score in all three groups being significantly less than that in the water control. Bone loss reduction for the minocycline group was significantly greater than that for any other group.

Topics: Alveolar Process; Aminacrine; Aminoacridines; Animals; Arvicolinae; Bacteria; Bone Resorption; Dental Caries; Female; Male; Minocycline; Periodontal Diseases; Rats; Sodium Fluoride; Streptococcus sanguis; Tetracyclines; Tooth Root