midostaurin and Lymphoma--Large-B-Cell--Diffuse

The phorbolester (TPA)-induced homotypic aggregation of human monoblastoid U-937 cells was completely abolished by staurosporine, but not by a new selective protein kinase C inhibitor, benzoylated staurosporine derivative CGP 41,251, a compound with known anti-tumor and drug resistance modulating activity. Staurosporine, a protein kinase inhibitor with broad profile of inhibitory activity on diverse protein kinases, but not CGP 41,251 substantially inhibited the TPA-induced up-regulation of intercellular adhesion molecule-1 (ICAM-1, CD54) and to a lesser extent also its ligand CD11a. These results suggest that protein kinase C-independent mechanisms, inhibited by staurosporine but not by the selective PKC inhibitor CGP 41,251 are involved in the TPA-induced homotypic adhesion and modulation of cell surface adhesion antigens in the human monoblastoid cell line U-937.

Topics: Alkaloids; Cell Aggregation; Humans; Intercellular Adhesion Molecule-1; Lymphoma, Large B-Cell, Diffuse; Protein Kinase C; Staurosporine; Tetradecanoylphorbol Acetate; Tumor Cells, Cultured