mhc-binding-peptide and Autoimmune-Diseases

mhc-binding-peptide has been researched along with Autoimmune-Diseases* in 2 studies

Reviews

1 review(s) available for mhc-binding-peptide and Autoimmune-Diseases

Article	Year
HLA class II peptide-binding and autoimmunity. Tissue antigens, 2002, Volume: 59, Issue:2 The HLA class II locus is located in the 6p21.3 region on the short arm of chromosome 6 and encompasses approximately 700 kb. It consists of over 30 gene loci including the major class II structural genes DP, DQ and DR. While autoimmune disease correlates to specific DP, DQ or DR alleles have been documented, due to the strong linkage disequilibrium between the different HLA alleles, especially between the DR and DQ, the precise identification of susceptible MHC alleles for a number of autoimmune diseases remains elusive. Topics: Antigen Presentation; Autoimmune Diseases; Autoimmunity; Genes, MHC Class II; Histocompatibility Antigens Class II; Humans; Immune Tolerance; Models, Immunological; Models, Molecular; Oligopeptides	2002

Article

Year

HLA class II peptide-binding and autoimmunity.

Tissue antigens, 2002, Volume: 59, Issue:2

The HLA class II locus is located in the 6p21.3 region on the short arm of chromosome 6 and encompasses approximately 700 kb. It consists of over 30 gene loci including the major class II structural genes DP, DQ and DR. While autoimmune disease correlates to specific DP, DQ or DR alleles have been documented, due to the strong linkage disequilibrium between the different HLA alleles, especially between the DR and DQ, the precise identification of susceptible MHC alleles for a number of autoimmune diseases remains elusive.

Topics: Antigen Presentation; Autoimmune Diseases; Autoimmunity; Genes, MHC Class II; Histocompatibility Antigens Class II; Humans; Immune Tolerance; Models, Immunological; Models, Molecular; Oligopeptides

2002

Other Studies

1 other study(ies) available for mhc-binding-peptide and Autoimmune-Diseases

Article	Year
[Application of MHC-Ig/peptide polymer in detecting antigen-specific cytotoxic T lymphocytes in mice with experimental autoimmune uveitis]. Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2010, Volume: 41, Issue:1 To explore the application of MHC-Ig/peptide polymer technique for detecting antigen-specific cytotoxic T lymphocytes (CTLs) in mice with experimental autoimmune uveitis (EAU).. B10RIII mice were immunized with an interphotoreceptor retinal-binding protein (IRBP) synthetic peptide (IRBP161-180). The in vivo primed T cells were separated and stained with MHC-Ig polymer combined with a panel of truncated peptides derived from IRBP161-180. The level of IRBP-specific CTLs cells was determined by FACS analysis. The CD8+ T cells were isolated from the primed T cells and stimulated with complex polymers containing MHC-Ig and various IRBP-derived peptides. The proliferation of CD8+ T cells was measured by H thymidine incorporation. The production of interferon- (IFN-) in the cell suspensions was measured by ELISA.. The IRBP-specific CTLs were detected by MHC-Ig/peptide polymers. The MHC-Ig/IRBP168-177 peptide polymer dtected 12.3% specific CTLs, showing greater ability in stimulating proliferation of CTLs and production of IFN--than the other MHC-Ig/ peptide polymers (P < 0.01). The truncated 10-mer peptide, IRBP168-177, was the major antigenic epitope for the IRBP-specific CTLs. The MHC-Ig/IRBP168-177 peptide polymer detected the highest level(4.9% +/- 1.1%) of specific CTLs from peripheral blood mononuclear cells (PBMCs) at the acute stage of EAU.. The MHC-Ig polymer technique is an effective instrument for detecting antigen-specific CTLs, with good sensitivity and specificity in EAU studies. Topics: Animals; Autoimmune Diseases; Eye Proteins; Female; Immunoglobulin G; Interferons; Mice; Mice, Mutant Strains; Oligopeptides; Photoreceptor Cells, Vertebrate; Polymers; Retinol-Binding Proteins; T-Lymphocytes, Cytotoxic; Uveitis	2010

Article

Year

[Application of MHC-Ig/peptide polymer in detecting antigen-specific cytotoxic T lymphocytes in mice with experimental autoimmune uveitis].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2010, Volume: 41, Issue:1

To explore the application of MHC-Ig/peptide polymer technique for detecting antigen-specific cytotoxic T lymphocytes (CTLs) in mice with experimental autoimmune uveitis (EAU).. B10RIII mice were immunized with an interphotoreceptor retinal-binding protein (IRBP) synthetic peptide (IRBP161-180). The in vivo primed T cells were separated and stained with MHC-Ig polymer combined with a panel of truncated peptides derived from IRBP161-180. The level of IRBP-specific CTLs cells was determined by FACS analysis. The CD8+ T cells were isolated from the primed T cells and stimulated with complex polymers containing MHC-Ig and various IRBP-derived peptides. The proliferation of CD8+ T cells was measured by H thymidine incorporation. The production of interferon- (IFN-) in the cell suspensions was measured by ELISA.. The IRBP-specific CTLs were detected by MHC-Ig/peptide polymers. The MHC-Ig/IRBP168-177 peptide polymer dtected 12.3% specific CTLs, showing greater ability in stimulating proliferation of CTLs and production of IFN--than the other MHC-Ig/ peptide polymers (P < 0.01). The truncated 10-mer peptide, IRBP168-177, was the major antigenic epitope for the IRBP-specific CTLs. The MHC-Ig/IRBP168-177 peptide polymer detected the highest level(4.9% +/- 1.1%) of specific CTLs from peripheral blood mononuclear cells (PBMCs) at the acute stage of EAU.. The MHC-Ig polymer technique is an effective instrument for detecting antigen-specific CTLs, with good sensitivity and specificity in EAU studies.

Topics: Animals; Autoimmune Diseases; Eye Proteins; Female; Immunoglobulin G; Interferons; Mice; Mice, Mutant Strains; Oligopeptides; Photoreceptor Cells, Vertebrate; Polymers; Retinol-Binding Proteins; T-Lymphocytes, Cytotoxic; Uveitis

2010