mezerein and Thymoma

mezerein has been researched along with Thymoma* in 1 studies

Other Studies

1 other study(ies) available for mezerein and Thymoma

ArticleYear
A rapid colorimetric microassay to detect agonists/antagonists of protein kinase C based on adherence of EL-4.IL-2 cells.
    The Journal of antibiotics, 1991, Volume: 44, Issue:1

    A rapid, colorimetric, microassay for detection of agents which are known agonists/antagonists of protein kinase C (PKC) was developed, utilizing their effects on adherence of EL-4.IL-2 cells. Cells that were incubated with agents which are known inducers of PKC activation, phorbol 12-myristate 13-acetate (PMA), phorbol 12,13-dibutyrate (PDBu), mezerein and indolactam V, readily adhered to wells of 96 well microtiter plates within 1-2 hours, whereas cells incubated with the negative PKC activator, 4 alpha-phorbol 12-myristate 13-acetate (4 alpha-PMA), which is structurally related to PMA (4 beta-PMA), did not adhere. The adherent cells withstood repeated vigorous washings with tissue culture medium. Adherence of EL-4.IL-2 cells in the presence of PMA could be blocked by the addition of two known inhibitors of PKC, 1-(5-isoquinoline-sulfonyl)-2-methylpiperazine dihydrochloride and staurosporine. Detection of the presence of adherent cells was accomplished by the addition of a tetrazolium salt to culture wells and determination of the remaining viable cells by scanning using a multiwell spectrophotometer (ELISA reader). The EL-4.IL-2 adherence assay meets several important criteria for use as a primary screen in the detection of potential PKC agonists/antagonists, i.e. its selectivity, simplicity, rapid performance through automation and reproducibility.

    Topics: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; Alkaloids; Animals; Carcinogens; Cell Adhesion; Colorimetry; Diterpenes; Dose-Response Relationship, Drug; Enzyme Activation; Indoles; Isoquinolines; Lactams; Phorbol 12,13-Dibutyrate; Phorbol Esters; Piperazines; Protein Kinase C; Reproducibility of Results; Staurosporine; Terpenes; Tetradecanoylphorbol Acetate; Thymoma; Tumor Cells, Cultured

1991