mezerein and Lymphoma--Large-B-Cell--Diffuse

M5076, a tumorigenic murine macrophage cell line, demonstrates diverse proliferative responses to a panel of protein kinase C activators. Thus, the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate and mezerein potently inhibit cellular proliferation (by greater than 90%), whereas the diacylglycerol analogue 1-oleoyl-2-acetyl-sn-glycerol markedly stimulates proliferation of serum-starved, quiescent M5076 cells. Another DG analogue, 1,2-dioctanoyl-sn-glycerol, has no effect on growth. However, all of these agents induce expression of c-fos oncogene mRNA levels to a similar magnitude and activate protein kinase C as determined by Mr 80,000 phosphorylation. Levels of c-fos protein induced by these treatments were markedly different with the antiproliferative agents producing greater c-fos protein levels.

Topics: Animals; Cell Division; Diglycerides; Diterpenes; DNA Replication; Female; Glycerides; Lymphoma, Large B-Cell, Diffuse; Macrophages; Mice; Mice, Inbred C57BL; Ovarian Neoplasms; Phorbol Esters; Protein Kinase C; Protein-Tyrosine Kinases; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-fos; Proto-Oncogenes; RNA, Messenger; Terpenes; Tumor Cells, Cultured