methylnitronitrosoguanidine and Tuberous-Sclerosis
methylnitronitrosoguanidine has been researched along with Tuberous-Sclerosis* in 4 studies
Other Studies
4 other study(ies) available for methylnitronitrosoguanidine and Tuberous-Sclerosis
Article | Year |
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Serum factors responsible for unusual induction of plasminogen activator activity in tuberous sclerosis.
Peripheral blood lymphocytes derived from tuberous sclerosis (TS) patients showed unusually high levels of plasminogen activator (PA) activity after treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Serum obtained from peripheral blood of TS patients also enhanced the PA activity level when normal control lymphocytes were incubated with the serum prior to MNNG treatment. Factors exhibiting the enhancing activity were eluted with a solution of about 0.70 M KCl on dye-ligand chromatography, which were inhibited on incubation with an anti-human interferon (HuIFN)-beta antibody, but not with anti-HuIFN-alpha or anti-HuIFN-gamma antibodies. Unlike in the case of HuIFN-beta, the eluted samples did not possess antiviral or anticellular activity. Thus, it seems likely that serum from TS patients contains factors which are responsible for the unusual PA induction and which have a similar epitope to HuIFN-beta. Topics: Adolescent; Adult; Antibodies; Antibodies, Monoclonal; Blood Physiological Phenomena; Child; Child, Preschool; Female; Humans; Interferon-alpha; Interferon-beta; Interferon-gamma; Lymphocytes; Male; Methylnitronitrosoguanidine; Plasminogen Activators; Reference Values; Tuberous Sclerosis | 1992 |
Enhancement of plasminogen activator activities by N-methyl-N'-nitro-N-nitrosoguanidine in tuberous sclerosis fibroblasts.
The alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) enhanced the levels of plasminogen activator (PA) activity in fibroblast cells derived from the skin of patients with tuberous sclerosis. The enhanced enzyme levels were not correlated with those of cloning efficiency nor those of DNA synthesis after MNNG treatment. Enzyme enhancement was also observed in fibroblasts of ataxia telangiectasia and in human neoplastic glia cells, but not in fibroblasts of normal children. The PA induction test may be sufficiently sensitive for the detection of the cellular defects of tuberous sclerosis. Topics: Cell Line; Cell Survival; DNA; Enzyme Activation; Enzyme Induction; Fibroblasts; Humans; Methylnitronitrosoguanidine; Plasminogen Activators; Tuberous Sclerosis | 1987 |
Patients with tuberous sclerosis have fibroblasts with normal limits for growth characteristics and sensitivities to DNA alkylating agents.
Topics: 4-Nitroquinoline-1-oxide; Adolescent; Adult; Cell Division; Cell Survival; Cells, Cultured; Child; DNA Repair; Ethyl Methanesulfonate; Fibroblasts; Humans; Methylnitronitrosoguanidine; Mitomycin; Mitomycins; Skin; Tuberous Sclerosis | 1984 |
Lymphoblastoid lines and skin fibroblasts from patients with tuberous sclerosis are abnormally sensitive to ionizing radiation and to a radiomimetic chemical.
Lymphoblastoid lines, derived by transforming peripheral blood lymphocytes with Epstein-Barr virus, and skin fibroblast lines were established from two patients with tuberous sclerosis. The number of viable lymphoblastoid cells was determined by their ability to exclude the vital dye trypan blue after their irradiation with x-rays or 254 nm ultraviolet light. The growth of fibroblasts was determined by their ability to form colonies after treatment with the radiomimetic, DNA-damaging chemical N-methyl-N'-nitro-N-nitrosoguanidine. The tuberous sclerosis lymphoblastoid lines were hypersensitive to x-rays but had normal sensitivity to the ultraviolet radiation. The tuberous sclerosis fibroblast lines were hypersensitive to the N-methyl-N'-nitro-N-nitrosoguanidine. The hypersensitivity of tuberous sclerosis cells to x-rays and to N-methyl-N'-nitro-N-nitrosoguanidine is believed to reflect defective repair of DNA damaged by these agents and may provide the basis for in vitro, including prenatal, diagnostic tests for tuberous sclerosis. Topics: Adolescent; Adult; Cell Survival; Cells, Cultured; Female; Fibroblasts; Humans; In Vitro Techniques; Lymphocytes; Male; Methylnitronitrosoguanidine; Skin; Tuberous Sclerosis; Ultraviolet Rays; X-Rays | 1982 |