methylnitronitrosoguanidine and Monosomy

methylnitronitrosoguanidine has been researched along with Monosomy* in 3 studies

Other Studies

3 other study(ies) available for methylnitronitrosoguanidine and Monosomy

ArticleYear
[Studies on mechanism of carcinogenesis in vitro. IV. Studies on relationship between frequency of chromosome 15 monosomy and malignant expression of different clones from malignantly transformed baby hamster lung cells (BHL B4)].
    Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae, 1990, Volume: 12, Issue:2

    The proceeding of malignantly transformed rodent cells in vitro is often accompanied by regular variation of chromosome numbers and structures. In order to clarify the relationship between them, we obtained five clones C1-1-5-from BHL B4, cell line, and analysed their biological characteristics. Results showed a positive correlation between frequency of chromosome 15 monosomy, it is suggested that suppressive gene of malignant phenotype be located on chromosome 15.

    Topics: Animals; Cell Line, Transformed; Cell Transformation, Neoplastic; Chromosome Deletion; Cricetinae; Fibroblasts; Gene Expression Regulation, Neoplastic; Lung; Mesocricetus; Methylnitronitrosoguanidine; Monosomy; Neoplasm Transplantation; Phenotype

1990
Studies on the relationship between chromosome No15 and the biological characteristics of malignant transformed Syrian hamster fibroblast cell lines.
    Proceedings of the Chinese Academy of Medical Sciences and the Peking Union Medical College = Chung-kuo i hsueh k'o hsueh yuan, Chung-kuo hsieh ho i k'o ta hsueh hsueh pao, 1990, Volume: 5, Issue:1

    During the course of malignant transformation of mammalian cells in vitro, a regular variation in chromosome number and structure is usually found. In order to elucidate the relationship between chromosomal changes and malignant expression, we isolated five clones from a malignant transformed Syrian hamster fibroblast cell line and analysed their biological characteristics, as well as chromosomal changes. A positive correlation between chromosome No 15 monosomy and the transformed and malignant phenotype was observed. We suggest that a suppression gene may be located on chromosome No 15, and its deletion or the loss of the chromosome may result in expression of the malignant phenotype.

    Topics: Animals; Cell Line, Transformed; Chromosome Deletion; Cricetinae; Fibroblasts; Fibrosarcoma; Gene Expression Regulation, Neoplastic; Lung; Mesocricetus; Methylnitronitrosoguanidine; Monosomy; Neoplasm Transplantation; Phenotype

1990
[Studies on mechanism of carcinogenesis in cell culture. III. Butyric acid-induced phenotypic reversion of malignant transformed Syrian hamster lung cells (BHLB4)].
    Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae, 1989, Volume: 11, Issue:3

    The MNNG transformed Syrian hamster lung fibroblast cell line (BHLB4) established in our laboratory was treated with 2.0 mmol/L butyric acid. We obtained a butyric acid-dependent partially reversed cell line (ButB4) similar to normal cells in morphology, membrane properties, proliferation rate, colony-forming ability on solid agar culture, etc. However, when transplanted into nude mice, this cell line's tumorigenicity remained intact. Detection of cAMP levels in the cytoplasm showed much higher levels in ButB4 cells than in transformed cells. Butyric acid activity is evidently associated with changes of cAMP. In addition to phenotypical analysis, the karyotypes of ButB4 and BHLB4 were analyzed in detail. It was found that the disomy rate of C15 was significantly increased in ButB4 cells (73%) as compared with BHLB4 cells (33%). These results show that butyric acid not only induces phenotypic reversion in BHLB4 cells directly, but can also selectively kill C15 monosomy cell clones, allowing rapid growth of C15 disomy cell clones. Butyric acid may also influence maintenance of the reversion phenotype.

    Topics: Animals; Butyrates; Cell Line, Transformed; Cell Transformation, Neoplastic; Cricetinae; Cyclic AMP; Fibroblasts; Lung; Mesocricetus; Methylnitronitrosoguanidine; Monosomy; Phenotype

1989
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