methylnitronitrosoguanidine and Hypersensitivity

The cross-sensitivity of X-ray-hypersensitive lung fibroblasts from LEC strain (LEC) rats to other DNA-damaging agents was examined. The LEC cells were 2- to 3-fold more sensitive to bleomycin (BLM) that induces DNA double-strand breaks, and to a cross-linking agent, mitomycin C, than the cells from WKAH strain (WKAH) rats, while they were slightly sensitive to alkylating agents, ethyl nitrosourea and N-methyl-N'-nitro-N-nitrosoguanidine, but not to UV-irradiation. Although no difference was observed in the initial yields of DNA double-strand breaks induced by BLM between LEC and WKAH cells, the repair process of DNA double-strand breaks was significantly slower in LEC cells than in WKAH cells.

Topics: Alkylating Agents; Animals; Antibiotics, Antineoplastic; Bleomycin; Cell Survival; Cells, Cultured; Cross Reactions; DNA; DNA Damage; Ethylnitrosourea; Fibroblasts; Hypersensitivity; Lung; Methylnitronitrosoguanidine; Mitomycin; Rats; Rats, Inbred Strains; Ultraviolet Rays; X-Rays