methylnitronitrosoguanidine and Cadmium-Poisoning

A series of programs applicable to in vitro test symptoms in environmental toxicology are described for the Tektronix 4050 series graphic system computer. The file structure, experimental design and identification, the program library, data entry program, and programs to compile data from separate experiments are presented. Experimental design information and test data are stored on magnetic tape. The programs are designed to compute cell number and viability, adenosine triphosphate level, and protein and DNA synthetic activity from raw data obtained from cellular toxicity experiments; and cloning efficiency, mutation yield, mutation frequency, and other parameters for mutagenicity. A set of subprograms can be used for statistical analysis of the data and for construction of frequency distributions. The applicability of the programs is illustrated by data obtained from exposure of Chinese hamster ovary cells to cadmium chloride or N-methyl-N'-nitro-N-nitroso-guanidine.

Topics: Animals; Cadmium Poisoning; Cells; Computers; Cricetinae; Cricetulus; Female; Methylnitronitrosoguanidine; Mutagenicity Tests; Software; Statistics as Topic; Toxicology