methylcellulose and Wounds-and-Injuries

Wounds that have stalled healing are costly in terms of patient morbidity and increase in use of material and financial resources. A natural polymer beta-glucans has been incorporated into a methylcellulose gel to provide a topical gel designed to accelerate healing in wounds where it has stalled. Although the gel provides an environment conducive to moist wound healing the active agent, beta-glucans, activate the innate immune response.. Using a Markov cohort simulation model, data were extrapolated from a double-blind randomised trial to evaluate the economic benefits of the soluble beta-glucan (SBG) gel in the treatment of diabetic foot ulcers (DFUs).. Over an annual budget cycle, SBG gel is expected to heal 94% of wounds compared with 78% when given standard care. It also healed wounds more quickly, with the average expected healed weeks 34.4 in the SBG gel group, compared with 24.7 methylcellulose dressing group. In our model this leads to a cost saving over an annual budget cycle of £503 per patient. Note: healed weeks refers to the number of weeks when the wound has healed during the 12-week period and should not be confused with weeks to healing.. The shorter healing time associated with the SBG gel treatment leads to a cost saving because fewer weeks of treatment are required to heal the wound, suggesting this is a promising new cost-effective option for the treatment of DFUs.

Topics: Administration, Cutaneous; Bandages; beta-Glucans; Cohort Studies; Computer Simulation; Cost-Benefit Analysis; Gels; Humans; Markov Chains; Methylcellulose; Randomized Controlled Trials as Topic; Treatment Outcome; Wound Healing; Wounds and Injuries

Extracellular matrix (ECM) provides structural support and biochemical cues for tissue development and regeneration. Here we report a thermosensitive hydrogel composed of soluble ECM (sECM) and methylcellulose (MC) for injectable stem cell delivery. The sECM was prepared by denaturing solid ECM extracted from human adipose tissue and then blended with a MC solution. At low temperatures, the sECM-MC solution displayed a viscous solution state in which the loss modulus (G″) was predominant over the storage modulus (G'). With increasing temperature, G' increased dramatically and eventually exceeded G″ around 34 °C, characteristic of the transition from a liquid-like state to an elastic gel-like state. After a single injection of the stem cell-embedded hydrogel in full thickness cutaneous wound, the wound healed rapidly through re-epithelialization and neovascularization with minimum scar formation. The overall results suggest that in-situ-forming sECM-MC hydrogels are a promising injectable vehicle for stem cell delivery and tissue regeneration.

Topics: Cells, Cultured; Extracellular Matrix; Humans; Hydrogels; Injections; Methylcellulose; Regeneration; Skin; Stem Cells; Temperature; Wounds and Injuries

Experimental studies of composite materials formed on the basis of fluorine-containing latex and bioactive polysaccharides showed that physicochemical properties of composite materials and their adhesion characteristics can be modulated by variations of polysaccharide-latex ratio and the nature of polysaccharides. The ratio of components ensuring the formation of biosynthetic films that meet the standards for modern wound coating and maintain adhesion and growth of substrate-dependent mammalian cells was determined. These materials can considerably increase the efficiency of treatment of extensive and deep skin wounds in cases when application of cell cultures is indicated.

Topics: Bandages; Biocompatible Materials; Cell Adhesion; Cell Culture Techniques; Humans; Latex; Materials Testing; Methylcellulose; Polysaccharides; Wounds and Injuries

A semi-solid albumin solder formulated with hydroxypropylmethylcellulose (HPMC) was designed to improve the characteristics of liquid and solid solders.. Acute tensile strengths were determined on canine small bowel in vitro by using liquid 50% bovine serum albumin (BSA), semi-solid 48% BSA with HPMC, and solid 60% BSA solder. Long-term healing of liquid and semi-solid solders, compared with a suture control, was evaluated in a porcine skin model, with tensile strength as well as histologic findings obtained on postoperative day 7.. Acutely, semi-solid solder demonstrated a significantly (P < 0.05) higher tensile strength when compared with liquid or solid solder. At 7 days, HSA semi-solid and BSA semi-solid had significantly (P < 0.05) higher tensile strength than suture control; however, no differences were seen for liquid or solid solder groups. No differences in histology were appreciable between any of the solder groups in a porcine skin model.. Acutely and at 7 days, semi-solid solder was stronger than 50% liquid albumin with better handling characteristics.

Topics: Animals; Dogs; Hemostatics; Hypromellose Derivatives; In Vitro Techniques; Intestine, Small; Laser Therapy; Methylcellulose; Serum Albumin, Bovine; Skin; Sutures; Swine; Tensile Strength; Tissue Adhesives; Wound Healing; Wounds and Injuries

Wound dressings may induce cytotoxic effects. In this study, we check several, mostly commercially available, wound dressings for cytotoxicity. We used our previously described, newly developed and highly sensitive 7 d methylcellulose cell culture with fibroblasts as the test system. Cytotoxicity is assessed by monitoring cell growth inhibition, supported by cell morphological evaluation using light and transmission electron microscopy. We tested conventional wound dressings, polyurethane-based films, composites, hydrocolloids and a collagen-based dressing. It was shown that only 5 out of 16 wound dressings did not induce cytotoxic effects. All 5 hydrocolloids were found to inhibit cell growth (greater than 70%), while cells had strongly deviant morphologies. The remaining wound dressings showed medium cytotoxic effects, with cell growth inhibition, which varied from low (+/- 15%), medium-low (+/- 25%) to medium-high (+/- 50%). Measurable cytotoxic effects of dressings detected in vitro are likely to interfere with wound healing when applied in vivo. The results are discussed in view of the clinical uses with contaminated wounds, impaired epithelialization or hypergranulation.

Topics: Bandages; Biocompatible Materials; Cell Division; Cells, Cultured; Fibroblasts; Humans; Materials Testing; Methylcellulose; Microscopy, Electron; Wound Healing; Wounds and Injuries