methylcellulose and Urinary-Bladder-Neoplasms

Adhesive bowel obstruction is one of the most frequent complications after radical cystectomy, prolonging hospital stay and fasting period and increasing medical expenses. This study evaluated the effectiveness of hyaluronic acid/carboxymethylcellulose (HA/CMC) in preventing adhesive bowel obstruction after laparoscopic radical cystectomy.. Randomized, controlled, single-blinded study was performed. Of 76 patients who underwent laparoscopic radical cystectomy for bladder cancer, 38 received HA/CMC instillation and 38 did not. The primary endpoint was the rate of postoperative adhesive bowel obstruction. The secondary endpoint was the rate of other postoperative outcomes.. None of the patients who received HA/CMC instillation experienced postoperative adhesive bowel obstructions, compared with six (15.79%) patients in the control group (p = 0.025). Of the six patients with ileus, two underwent adhesiolysis. There were no significant differences between the two groups in other postoperative outcomes.. HA/CMC instillation during laparoscopic radical cystectomy may reduce the incidence of postoperative adhesive bowel obstruction without adverse effects.

Topics: Aged; Cystectomy; Female; Humans; Hyaluronic Acid; Intestinal Obstruction; Intraoperative Care; Laparoscopy; Male; Methylcellulose; Postoperative Complications; Single-Blind Method; Treatment Outcome; Urinary Bladder Neoplasms

Because in vitro cell growth of transitional cell carcinoma explants and cell lines often fail to adequately proliferate in semisolid media, we have examined the effect of agents used to make media semisolid (methyl cellulose, Bacto-agar, Sea Plaque agarose and Sea Prep 15/45 agarose) on the in vitro growth of 11 transitional cell carcinoma cell lines. The growth of human transitional cell carcinoma lines was supported such that agents permissive for growth ranked as follows: Sea Plaque agarose approximately Sea Prep agarose greater than methyl cellulose greater than Bacto-agar. These observations have important implications for the in vitro study of transitional cell carcinoma cell lines and are relevant to the development of improved chemosensitivity determinations for human transitional cell carcinoma.

Topics: Agar; Carcinoma, Transitional Cell; Cell Division; Cell Line; Culture Media; Humans; In Vitro Techniques; Methylcellulose; Sepharose; Urinary Bladder; Urinary Bladder Neoplasms

The effects of different agars (Bacto-agar and deoxycholate lactose agar), agaroses (LE, ME, Sea Plaque and Sea Prep 15/45) and methyl cellulose on the growth of a human tumor cell line, derived from a transitional cell carcinoma of the urinary bladder, were examined. The overall growth in the presence of agars and agarose was generally less than in liquid medium alone. In contrast, growth in the presence of methyl cellulose was significantly enhanced. Thus, methyl cellulose may be a useful agent for optimizing the proliferation of primary tissue cultures prepared from human transitional cell carcinomas.

Topics: Agar; Carcinoma, Transitional Cell; Cell Division; Cell Line; Culture Media; Humans; Methylcellulose; Sepharose; Urinary Bladder Neoplasms

Topics: Agar; Carcinoma; Cell Division; Cell Survival; Cells, Cultured; Clone Cells; Drug Evaluation, Preclinical; Humans; Karyotyping; Methylcellulose; Mitomycins; Urinary Bladder Neoplasms

We report the development of a clonogenic assay for progenitor cells in transitional cell carcinoma of the bladder. Colony growth has been demonstrated from cells obtained both from surgical biopsies and from bladder barbotages. Electron microscopic and karyotypic evidence supports the contention that these progenitors represent a part of the population maintaining the tumor in vivo. Colony growth occurred in 9 of 11 surgical biopsy samples and in 6 of 6 bladder barbotage samples. Plating efficiency ranged up to 0.7%, and colony size was in some instances greater than 1000 cells. The assay appears potentially useful for analysis of the biology of human transitional cell carcinoma.

Topics: Agar; Aged; Carcinoma, Transitional Cell; Cell Division; Chromosome Aberrations; Clone Cells; Female; Humans; Male; Methods; Methylcellulose; Microscopy, Electron; Middle Aged; Urinary Bladder Neoplasms

Topics: Aged; Barium Sulfate; Carbon Dioxide; Contrast Media; Cystoscopy; Fluoroscopy; Humans; Male; Methods; Methylcellulose; Mucous Membrane; Prostatic Hyperplasia; Urinary Bladder; Urinary Bladder Neoplasms; Urinary Catheterization; Urography